Abstract: A HUD calibration system is provided and includes a HUD, a reticle reference object, a target, and a control module. The HUD displays a virtual image including a first center feature and first features. The reticle reference object is physically fixed to a windshield of a vehicle. The target is physically disposed forward of the vehicle and includes a second center feature and second features. The control module: controls the HUD to display the virtual image superimposed over the target; perform a boresighting process to align a center of a cross-hair of the reticle reference object with the first and second center features and generate first reference values; perform an undistortion calibration process to rotate and undistort the virtual image relative to the target and generate second reference values; and adjust operation of the HUD based on the first and second reference values.