Patents by Inventor Paul C. Blainey

Paul C. Blainey has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220299511
    Abstract: Provided herein, in some embodiments, are methods for analyzing immune cells in a blood sample from a subject having, suspected of having, or being at risk for bacterial sepsis. The present disclosure is based, at least in part, on the finding that certain immune cells are expanded in subjects having sepsis compared to healthy subjects.
    Type: Application
    Filed: June 17, 2020
    Publication date: September 22, 2022
    Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology, The General Hospital Corporation
    Inventors: Miguel Reyes, Nir Hacohen, Paul C. Blainey, Michael R. Filbin, Marcia B. Goldberg, Roby P. Bhattacharyya
  • Patent number: 11136621
    Abstract: A kit includes a microfabricated device having a top surface defining an array of microwells for receiving a sample comprising at least one cell, and a membrane for applying on the top surface of the microfabricated device to retain the at least one cell in at least one microwell of the array of microwells after the sample is loaded on the microfabricated device.
    Type: Grant
    Filed: December 27, 2018
    Date of Patent: October 5, 2021
    Assignee: GENERAL AUTOMATION LAB TECHNOLOGIES INC.
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Patent number: 11104940
    Abstract: A method of screening for at least one biological entity of interest using a microfabricated device which has a top surface defining an array of microwells. A sample is loaded onto the microfabricated device such that at least one microwell of the array of microwells includes at least one cell and an amount of a nutrient. A membrane is applied to the microfabricated device to retain the at least one cell and the nutrient. A plurality of cells is cultured from the at least one cell in the at least one microwell of the array of microwells. The plurality of cells in the at least one microwell of the array of microwells are split into a first portion of the plurality of cells and a second portion of the plurality of cells. The plurality of cells is analyzed to determine a presence or absence of a biological entity of interest.
    Type: Grant
    Filed: December 27, 2018
    Date of Patent: August 31, 2021
    Assignee: GENERAL AUTOMATION LAB TECHNOLOGIES INC.
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Patent number: 11104939
    Abstract: A method of screening for at least one biological entity of interest using a microfabricated device which has a top surface defining an array of microwells. A sample is loaded onto the microfabricated device such that at least one microwell of the array of microwells includes at least one cell and an amount of a nutrient. A membrane is applied to the microfabricated device to retain the at least one cell and the nutrient. A plurality of cells are cultured from the at least one cell in the at least one microwell of the array of microwells. The plurality of cells is then analyzed to determine a presence or absence of a biological entity of interest.
    Type: Grant
    Filed: December 27, 2018
    Date of Patent: August 31, 2021
    Assignee: GENERAL AUTOMATION LAB TECHNOLOGIES INC.
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Patent number: 10900073
    Abstract: A microfabricated device defining a high density array of microwells is described for cultivating cells from a sample. A series of unique tags may be disposed in the microwells to identify one or more species of cells and locate the particular microwells in which each species was cultivated. A unique tag may be a nucleic acid molecule including a target-specific nucleotide sequence for annealing to a target nucleic acid fragment and a location-specific nucleotide sequence predetermined to identify one or more microwells. The device may be incubated to grow a plurality of cells, which may be split into an analysis portion and a reserve portion. High throughput methods are described for cultivating, screening, and determining a relative and/or absolute abundance of cells from a sample.
    Type: Grant
    Filed: April 21, 2016
    Date of Patent: January 26, 2021
    Assignee: GENERAL AUTOMATION LAB TECHNOLOGIES INC.
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Publication number: 20190194735
    Abstract: A method of screening for at least one biological entity of interest using a microfabricated device which has a top surface defining an array of microwells. A sample is loaded onto the microfabricated device such that at least one microwell of the array of microwells includes at least one cell and an amount of a nutrient. A membrane is applied to the microfabricated device to retain the at least one cell and the nutrient. A plurality of cells is cultured from the at least one cell in the at least one microwell of the array of microwells. The plurality of cells in the at least one microwell of the array of microwells are split into a first portion of the plurality of cells and a second portion of the plurality of cells. The plurality of cells is analyzed to determine a presence or absence of a biological entity of interest.
    Type: Application
    Filed: December 27, 2018
    Publication date: June 27, 2019
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Publication number: 20190194734
    Abstract: A method of screening for at least one biological entity of interest using a microfabricated device which has a top surface defining an array of microwells. A sample is loaded onto the microfabricated device such that at least one microwell of the array of microwells includes at least one cell and an amount of a nutrient. A membrane is applied to the microfabricated device to retain the at least one cell and the nutrient. A plurality of cells are cultured from the at least one cell in the at least one microwell of the array of microwells. The plurality of cells is then analyzed to determine a presence or absence of a biological entity of interest.
    Type: Application
    Filed: December 27, 2018
    Publication date: June 27, 2019
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Publication number: 20190194736
    Abstract: A kit includes a microfabricated device having a top surface defining an array of microwells for receiving a sample comprising at least one cell, and a membrane for applying on the top surface of the microfabricated device to retain the at least one cell in at least one microwell of the array of microwells after the sample is loaded on the microfabricated device.
    Type: Application
    Filed: December 27, 2018
    Publication date: June 27, 2019
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Patent number: 10227640
    Abstract: A method of screening for at least one biological entity of interest using a microfabricated device which has a top surface defining an array of microwells. A sample is loaded onto the microfabricated device such that at least one microwell of the array of microwells includes at least one cell and an amount of a nutrient. A membrane is applied to the microfabricated device to retain the at least one cell and the nutrient. Without furnishing additional nutrient to the microwells, the microfabricated device is incubated to grow a plurality of cells from the at least one cell in the at least one microwell of the array of microwells. The plurality of cells is then analyzed to determine a presence or absence of a biological entity of interest.
    Type: Grant
    Filed: September 30, 2017
    Date of Patent: March 12, 2019
    Assignee: GENERAL AUTOMATION LAB TECHNOLOGIES, INC.
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Publication number: 20180051327
    Abstract: A method of screening for at least one biological entity of interest using a microfabricated device which has a top surface defining an array of microwells. A sample is loaded onto the microfabricated device such that at least one microwell of the array of microwells includes at least one cell and an amount of a nutrient. A membrane is applied to the microfabricated device to retain the at least one cell and the nutrient. Without furnishing additional nutrient to the microwells, the microfabricated device is incubated to grow a plurality of cells from the at least one cell in the at least one microwell of the array of microwells. The plurality of cells is then analyzed to determine a presence or absence of a biological entity of interest.
    Type: Application
    Filed: September 30, 2017
    Publication date: February 22, 2018
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock
  • Publication number: 20160312275
    Abstract: A microfabricated device defining a high density array of microwells is described for cultivating cells from a sample. A series of unique tags may be disposed in the microwells to identify one or more species of cells and locate the particular microwells in which each species was cultivated. A unique tag may be a nucleic acid molecule including a target-specific nucleotide sequence for annealing to a target nucleic acid fragment and a location-specific nucleotide sequence predetermined to identify one or more microwells. The device may be incubated to grow a plurality of cells, which may be split into an analysis portion and a reserve portion. High throughput methods are described for cultivating, screening, and determining a relative and/or absolute abundance of cells from a sample.
    Type: Application
    Filed: April 21, 2016
    Publication date: October 27, 2016
    Inventors: Paul C. Blainey, Michael W. Seely, Roman Stocker, Karsten Zengler, Scott Conradson, Peter Christey, Alexander Hallock