Abstract: A semi-quantitative, immunochromatographic dual test device for the simultaneous detection of HIV/AIDS immune status CD4+ T cell equivalents, such as soluble CD40 ligand/CD 154, and the detection of an HIV antibody, includes one or more support materials capable of providing lateral flow. The one or more support materials include at least one sample receiving area for receiving a biological sample containing a first target analyte, the first target analyte being a CD4+ T cell equivalent, such as soluble CD40 ligand/CD 154, and a second target analyte, the second target analyte being an HIV antibody.

Abstract: A semi-quantitative, immunochromatographic device for the detection of HIV/AIDS immune status CD4+ T cell equivalents, such as soluble CD40 ligand/CD 154, includes one or more support materials capable of providing lateral flow. The one or more support materials include a first area for receiving a biological sample containing a target analyte, the analyte being a CD4+ T cell equivalent, such as soluble CD40 ligand/CD 154, a second area having a movably contained detector ligand, wherein the detector ligand is capable of forming a mobile complex with the soluble CD40 ligand/CD 154, and at least one capture area having a predetermined amount of an immobile capture reagent, the immobile capture reagent capable of specifically binding to the mobile complex formed by the soluble CD40 ligand/CD 154 protein and the detector ligand and providing a visible signal.