Abstract: This invention relates to an novel Gluconobacter oxydans 2-ketoreductase useful for the synthesis of chiral alcohols. Also related are isolated nucleic acids encoding G. oxydans 2-ketoreductase enzymes, and enzyme fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated G. oxydans 2-ketoreductase polypeptides, and fragments and variants thereof, and antibodies that specifically bind to G. oxydans 2-ketoreductase polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated G. oxydans 2-ketoreductase nucleic acids, polypeptides, and antibodies, and methods of using G. oxydans 2-ketoreductase in various reactions for industrial or pharmaceutical applications.

Abstract: The present invention provides a manufacturing method for the production of peptides that are grown in insect cell lines. The peptides are grown in insect cell cultures that are infected with baculovirus particles in a culture supplemented with a lipid mixture. The peptides are then isolated from the insect cell culture using a method that employs a tangential flow filtration cascade. The isolated peptides are glycopeptides having an insect specific glycosylation pattern. The glycopeptides may then be conjugated to a modifying group via linkage through a glycosyl linking group interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from glycosylated peptides by the action of a glycosyltransferase.

Abstract: This invention relates to a recombinant Pichia pastoris formate dehydrogenase (FDH) enzyme that catalyzes the oxidation of formate to carbon dioxide and the simultaneous reduction of nicotinamide adenine dinucleotide (NAD+) to its reduced form (NADH). Also related are isolated nucleic acids encoding P. pastoris FDH polypeptides, and fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated, recombinant P. pastoris FDH polypeptides, and fragments and variants thereof, and antibodies that specifically bind to P. pastoris FDH polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated P. pastoris FDH nucleic acids, polypeptides, and antibodies, and methods of using P. pastoris FDH in various reactions for industrial or pharmaceutical applications.

Abstract: A two stage feeding apparatus and method for feeding articles to a printing machine are disclosed. The apparatus comprises a transfer tray between a conventional stack loading mechanism and a conventional printing machine feeding mechanism. The transfer tray is both movable between a horizontal loading position and an inclined transitional position, and rotatable between the inclined transitional position and an inclined unloading position. The method comprises two stages of which a first stage involves moving the transfer tray between the horizontal loading position and the inclined transitional position, and a second stage involves rotating the transfer tray between the inclined transitional position and the inclined unloading position.

Abstract: Apparatus for supplying rows of stacked frusto-conical cups to a feeding assembly of a cup printing apparatus includes a rotatable, hollow, crossed turret comprised of an outer turret section formed by four radially and equiangularly oriented connected tubes, and a central tubular section positioned centrally within the outer turret section, the crossed turret supplying a first row of stacked cups to the feeding assembly and automatically supplying a subsequent row of stacked cups in stacked relation on the first row after a level of the first row has decreased to a lower predetermined level and before a last cup of the first row has been supplied on a respective mandrel assembly of the cup printing apparatus; a rotary actuator which rotates the central tubular section; a motor driven assembly which rotates the outer turret section; a first sensor which senses when the level of the first row has decreased to the lower predetermined level to actuate the motor driven assembly to cause the outer turret section a