Abstract: Methods of disrupting germ cell migration and development in a fish embryo by inducing targeted expression of Sdf-1a or Lif and disruption of the Sdf-1a gradient or a Lif signaling pathway in the fish embryo have been developed. Plasmid constructs containing a gene encoding Sdf-1a or Lif and a gene encoding a signaling sequence for targeted expression of Sdf-1a or Lif have been generated. The plasmids will be administered to a fish or a population of fish to reproductively sterilize the population with efficacy of up to 100%. Transgenic fish of this invention are reproductively incompetent of genetically contaminating a wild fish population.

Abstract: Methods of disrupting germ cell migration and development in a fish embryo by inducing targeted expression of Sdf-1 a or Lif and disruption of the Sdf-1 a gradient or a Lif signaling pathway in the fish embryo have been developed. Plasmid constructs containing a gene encoding Sdf-1 a or Lif and a gene encoding a signaling sequence for targeted expression of Sdf-1 a or Lif have been generated. The plasmids will be administered to a fish or a population of fish to reproductively sterilize the population with efficacy of up to 100%. Transgenic fish of this invention are reproductively incompetent of genetically contaminating a wild fish population.

Abstract: The present invention includes methods for integrating a modifying polynucleotide into a target polynucleotide in a cell, or instance, a fish cell or a mouse cell, and methods for making chimeric animals, heterozygous animals, and homozygous animals that are chimeric, heterozygous, or homozygous, respectively, for a modifying polynucleotide integrated into a target polynucleotide. The present invention also includes vectors and methods of using the vectors for integrating a modifying polynucleotide into a target polynucleotide in a cell, for instance, a fish cell or a mouse cell.

Abstract: The present invention is directed to methods of making a fish embryo cell line that can give rise to germ cells when introduced to a fish embryo. The present invention is further directed to methods of using the fish embryo cell line, making germ line chimeric fish, and to a cell culture medium.