Patents by Inventor PAUL DEAR
PAUL DEAR has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10690689Abstract: A microfluidic sequencing device in which a stream of microdroplets at least some of which contain a single nucleotide base are made to undergo reaction with a capture system to capture and detect an ordered sequence of single nucleotide bases generated by progressive pyrophosphorolysis.Type: GrantFiled: October 5, 2017Date of Patent: June 23, 2020Assignees: BASE4 INNOVATION LTD, UNITED KINGDOM RESEARCH AND INNOVATIONInventors: Cameron Alexander Frayling, Barnaby Balmforth, Bruno Flavio Nogueira de Sousa Soares, Thomas Henry Isaac, Boris Breiner, Alessandra Natale, Michele Amasio, Paul Dear
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Patent number: 10551399Abstract: A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterised by the steps of (1) generating a stream of single nucleotide bases from the analyte by pyrophosphorolysis; (2) producing captured molecules by reacting each single nucleotide base with a capture system labelled with detectable elements in an undetectable state; (3) releasing the detectable elements from each captured molecule in a detectable state and (4) detecting the detectable elements so released and determining the sequence of nucleotide bases therefrom. The method can be used advantageously in sequencers involving the use of microdroplets.Type: GrantFiled: October 5, 2017Date of Patent: February 4, 2020Assignees: BASE4 INNOVATION LTD, UNITED KINGDOM RESEARCH AND INNOVATIONInventors: Cameron Alexander Frayling, Barnaby Balmforth, Bruno Flavio Nogueira de Sousa Soares, Thomas Henry Isaac, Boris Breiner, Alessandra Natale, Michele Amasio, Paul Dear
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Patent number: 10480024Abstract: A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterised by the steps of (1) generating a stream of single nucleotide bases from the analyte; (2) producing captured molecules by reacting each single nucleotide base with a capture system; (3) amplifying at least part of the captured molecule to produce a plurality of amplicons characteristic of the single nucleotide base; (4) labelling the amplicons with a corresponding probe having a characteristic detectable element and (5) detecting a property characteristic of the detectable element.Type: GrantFiled: April 9, 2014Date of Patent: November 19, 2019Assignees: BASE4 INNOVATION LTD, UNITED KINGDOM RESEARCH AND INNOVATIONInventors: Cameron Alexander Frayling, Barnaby Balmforth, Bruno Flavio Nogueira de Sousa Soares, Thomas Henry Isaac, Boris Breiner, Alessandra Natale, Michele Amasio, Paul Dear
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Publication number: 20190185921Abstract: Various method of isolating a modified polynucleotide having the formula A-P-B from a crude mixture of modified polynucleotides also including those having the formulae A-P-A and B-P-B, wherein P is a polynucleotide region and A and B are different modifying moieties or nucleotide sequences are provided. The methods include the steps of (a) reacting the crude mixture concurrently or consecutively with beads of one type capable of binding to moiety A but not B, and beads of a second type capable of binding to moiety B but not A; (b) fractionating the intermediate product based on the properties of each type of bead and of pairs of different types of bead conjoined by A-P-B polynucleotides, such that only or predominantly conjoined pairs of the two different types of bead are retained and (c) optionally, releasing one or both beads from such conjoined pairs such that the polynucleotide may be recovered.Type: ApplicationFiled: June 14, 2017Publication date: June 20, 2019Applicant: BASE4 INNOVATION LTDInventor: Paul DEAR
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Publication number: 20180044728Abstract: A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterised by the steps of (1) generating a stream of single nucleotide bases from the analyte by pyrophosphorolysis; (2) producing captured molecules by reacting each single nucleotide base with a capture system labelled with detectable elements in an undetectable state; (3) releasing the detectable elements from each captured molecule in a detectable state and (4) detecting the detectable elements so released and determining the sequence of nucleotide bases therefrom. The method can be used advantageously in sequencers involving the use of microdroplets.Type: ApplicationFiled: October 5, 2017Publication date: February 15, 2018Applicants: BASE4 INNOVATION LTD, MEDICAL RESEARCH COUNCILInventors: Cameron Alexander FRAYLING, Barnaby BALMFORTH, Bruno Flavio Nogueira de Sousa SOARES, Thomas Henry ISAAC, Boris BREINER, Alessandra NATALE, Michele AMASIO, Paul DEAR
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Publication number: 20180044729Abstract: A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterised by the steps of (1) generating a stream of single nucleotide bases from the analyte by pyrophosphorolysis; (2) producing captured molecules by reacting each single nucleotide base with a capture system labelled with detectable elements in an undetectable state; (3) releasing the detectable elements from each captured molecule in a detectable state and (4) detecting the detectable elements so released and determining the sequence of nucleotide bases therefrom. The method can be used advantageously in sequencers involving the use of microdroplets.Type: ApplicationFiled: October 5, 2017Publication date: February 15, 2018Applicants: BASE4 INNOVATION LTD, MEDICAL RESEARCH COUNCILInventors: Cameron Alexander FRAYLING, Barnaby BALMFORTH, Bruno Flavio Nogueira de Sousa SOARES, Thomas Henry ISAAC, Boris BREINER, Alessandra NATALE, Michele AMASIO, Paul DEAR
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Patent number: 9828631Abstract: A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterized by the steps of (1) generating a stream of single nucleotide bases from the analyte by pyrophosphorolysis; (2) producing captured molecules by reacting each single nucleotide base with a capture system labelled with detectable elements in an undetectable state; (3) releasing the detectable elements from each captured molecule in a detectable state and (4) detecting the detectable elements so released and determining the sequence of nucleotide bases therefrom. The method can be used advantageously in sequencers involving the use of microdroplets.Type: GrantFiled: April 9, 2014Date of Patent: November 28, 2017Assignees: BASE4 INNOVATION LTD, MEDICAL RESEARCH COUNCILInventors: Cameron Alexander Frayling, Barnaby Balmforth, Bruno Flavio Nogueira de Sousa Soares, Thomas Henry Isaac, Boris Breiner, Alessandra Natale, Michele Amasio, Paul Dear
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Publication number: 20160040224Abstract: A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterised by the steps of (1) generating a stream of single nucleotide bases from the analyte by pyrophosphorolysis; (2) producing captured molecules by reacting each single nucleotide base with a capture system labelled with detectable elements in an undetectable state; (3) releasing the detectable elements from each captured molecule in a detectable state and (4) detecting the detectable elements so released and determining the sequence of nucleotide bases therefrom. The method can be used advantageously in sequencers involving the use of microdroplets.Type: ApplicationFiled: April 9, 2014Publication date: February 11, 2016Applicants: MEDICAL RESEARCH COUNCIL, BASE4 INNOVATION LTDInventors: Cameron Alexander FRAYLING, Barnaby BALMFORTH, Bruno Flavio Nogueira de Sousa SOARES, Thomas Henry ISAAC, Boris BREINER, Alessandra NATALE, Michele AMASIO, Paul DEAR
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Publication number: 20160040223Abstract: A method for determining the sequence of nucleotide bases in a polynucleotide analyte is provided. It is characterised by the steps of (1) generating a stream of single nucleotide bases from the analyte; (2) producing captured molecules by reacting each single nucleotide base with a capture system; (3) amplifying at least part of the captured molecule to produce a plurality of amplicons characteristic of the single nucleotide base; (4) labelling the amplicons with a corresponding probe having a characteristic detectable element and (5) detecting a property characteristic of the detectable element.Type: ApplicationFiled: April 9, 2014Publication date: February 11, 2016Applicants: MEDICAL RESEARCH COUNCIL, BASE4 INNOVATION LTDInventors: Cameron Alexander FRAYLING, Barnaby BALMFORTH, Bruno Flavio Nogueira de Sousa SOARES, Thomas Henry ISAAC, Boris BREINER, Alessandra NATALE, Michele AMASIO, Paul DEAR
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Publication number: 20050003379Abstract: The invention provides a method for identifying markers for use in mapping strategies, wherein the markers are derived from the mapping panel itself.Type: ApplicationFiled: December 18, 2003Publication date: January 6, 2005Inventors: Paul Dear, Madane Thangavelu, Alan Bankier
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Publication number: 20040005914Abstract: The present invention relates to an apparatus to provide a personal communication device consisting of one or more microcircuits deposited onto a man portable substrate, said microcircuits including a voice recognition input means in order to input data, commands or messages by voice, means to configure said voice inputted data, command or message to be transmitted to another device, wireless transmitter means for transmitting said data, command or message to said other device, wireless receiver means for receiving data or messages from said other device and means to communicate data or messages received from said other device to the user. In the preferred embodiment the wireless transmitter means and wireless receiver means are embodied in a radio-frequency modem. This radio frequency modem preferably uses Bluetooth architecture.Type: ApplicationFiled: July 8, 2002Publication date: January 8, 2004Inventor: Jean Paul Dear
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Publication number: 20010023012Abstract: A gel for analysis of DNA samples by electrophoresis is accommodated in the channels of a wall structure (1) formed by an extruded plastics material such as that known as Correx. For electrophoresis treatment with the plastics wall structure (1) maintained vertical in an electrophoresis tank, the sample for analysis may be added to the open top of a channel partially filled with gel (6). The wall structure (1) may have a transverse removable strip (14) giving access to the gel-filled channels, for electrophoresis with the wall structure (1) horizontal.Type: ApplicationFiled: December 14, 1998Publication date: September 20, 2001Inventors: PAUL DEAR, ALAN THOMAS BANKIER, MICHAEL BRUCE PIPER