Abstract: A cell culture substrate is provided that includes a substrate lattice having an ordered array of fibers and pores disposed between the fibers. The ordered array of fibers includes a cell culture surface to support adherent or semi-adherent cells during cell culture. The cell culture substrate further includes a positive charge coating disposed on the cell culture surface to promote adhesion of cells to the cell culture surface.

Abstract: A spheroid cell culture article including: a frame having a chamber including: an opaque side wall surface; a top aperture; a gas-permeable, transparent bottom; and optionally a chamber annex surface and second bottom, and at least a portion of the transparent bottom includes at least one concave arcuate surface, is disclosed. Methods of making and using the article are also disclosed.

Abstract: A spheroid cell culture article including: a chamber including: an opaque side wall surface; a top aperture; and a gas-permeable, liquid impermeable bottom including at least one concave surface, wherein at least a portion of the bottom is transparent, wherein at least a portion of the bottom includes a low-adhesion or no-adhesion material in or on the at least one concave surface, and wherein the gas permeable, liquid impermeable bottom includes polystyrene.

Abstract: A spheroid cell culture article including: a frame having a chamber including: an opaque side wall surface; a top aperture; a gas-permeable, transparent bottom; and optionally a chamber annex surface and second bottom, and at least a portion of the transparent bottom includes at least one concave arcuate surface, is disclosed. Methods of making and using the article are also disclosed.

Abstract: A spheroid cell culture article including: a frame having a chamber including: an opaque side wall surface; a top aperture; a gas-permeable, transparent bottom; and optionally a chamber annex surface and second bottom, and at least a portion of the transparent bottom includes at least one concave arcuate surface, is disclosed. Methods of making and using the article are also disclosed.

Abstract: Disclosed herein are functionalized cationic peptide monomers: peptide chains of two or more positively charged amino acids chosen from lysine or arginine, or derivatives thereof, which are functionalized, meaning that they are bound to one or more polymerization moieties. The functionalized cationic peptide monomers can be described by the formula Z-Xaan-Z1n1 wherein Z and Z1 are polymerization moieties and n1 is an integer of 0 or 1; Xaa is each independently an amino acid Lys or Arg and n is an integer from 2 to 10 and wherein at least one Xaa amino acid of Xaa is Lys, wherein the carboxyl terminus of the amino acid sequence is amidated. Functionalized cationic peptide monomers can be combined and polymerized to form cell culture surfaces.

Abstract: A spheroid cell culture article including: a frame having a chamber including: an opaque side wall surface; a top aperture; a gas-permeable, transparent bottom; and optionally a chamber annex surface and second bottom, and at least a portion of the transparent bottom includes at least one concave arcuate surface, is disclosed. Methods of making and using the article are also disclosed.

Abstract: Disclosed herein are functionalized cationic peptide monomers: peptide chains of two or more positively charged amino acids chosen from lysine or arginine, or derivatives thereof, which are functionalized, meaning that they are bound to one or more polymerization moieties. The functionalized cationic peptide monomers can be described by the formula Z-Xaan-Z1n1 wherein Z and Z1 are polymerization moieties and n1 is an integer of 0 or 1; Xaa is each independently an amino acid Lys or Arg and n is an integer from 2 to 10 and wherein at least one Xaa amino acid of Xaa is Lys, wherein the carboxyl terminus of the amino acid sequence is amidated. Functionalized cationic peptide monomers can be combined and polymerized to form cell culture surfaces.