Abstract: Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N-1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N-1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.

Abstract: Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.

Abstract: Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N?1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N?1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.

Abstract: Described are various embodiments of an ion beam chamber fluid delivery system and method for delivering a fluid onto a substrate in an ion beam system during operation. In one embodiment, the system comprises: a chamber comprising an ion beam gun oriented so as to cause ions to impinge the substrate, said chamber having a fluid delivery conduit therein for delivering the fluid into the chamber; a transferable substrate stage for holding the substrate, the transferable stage further configured to move between an operating position and a payload position during non-operation, said payload position for receiving and removing said substrate; and a fluid delivery nozzle being in a fixed location relative to the transferable stage, at least during operation, with an outlet position that is configured to deliver a fluid to a predetermined location on said transferable stage.

Abstract: Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.

Abstract: Systems and methods for determining similarities between an input data set and a target data set with the data sets being vector representations of the features of a candidate potential copy and a target original. A feature extraction module receives an image of the potential copy and extracts the features of that candidate. The features of the target original may already be extracted or may be separately extracted. The resulting data sets for the candidate and the original are then passed through a decision module. The decision module determines a level of similarity between the features of the candidate and the features of the original. The output of the decision module provides an indication of this level of similarity and, depending on this level of similarity, an alert may be generated. A report module may be included to provide an explanation regarding the level of similarity.

Abstract: Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.

Abstract: Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.

Abstract: Described are various embodiments of an ion beam chamber fluid delivery system and method for delivering a fluid onto a substrate in an ion beam system during operation. In one embodiment, the system comprises: a chamber comprising an ion beam gun oriented so as to cause ions to impinge the substrate, said chamber having a fluid delivery conduit therein for delivering the fluid into the chamber; a transferable substrate stage for holding the substrate, the transferable stage further configured to move between an operating position and a payload position during non-operation, said payload position for receiving and removing said substrate; and a fluid delivery nozzle being in a fixed location relative to the transferable stage, at least during operation, with an outlet position that is configured to deliver a fluid to a predetermined location on said transferable stage.

Abstract: Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N?1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N?1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.

Abstract: Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N-1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N-1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.

Abstract: The invention provides, in some aspects, methods of optimizing an app for execution on a client device by identifying components of the app used in such execution by a user having a designated role who accesses the app for download via a designated portal. A component can be, for example, a source code file or portion thereof, including, for example, a portion defining a widget or other user interface element. For each component, the method (i) identifies source code files on which execution of that component depends, e.g., in connection with execution of the particular app by the particular user, and (ii) generates a dependency-ordered stack of the source code files for that component. In further accord with those aspects of the invention, the method combines the dependency-ordered stacks into a single such stack and transfers the source code in an order specified by that stack to the client device for loading and execution.

Abstract: Systems and methods for determining similarities between an input data set and a target data set with the data sets being vector representations of the features of a candidate potential copy and a target original. A feature extraction module receives an image of the potential copy and extracts the features of that candidate. The features of the target original may already be extracted or may be separately extracted. The resulting data sets for the candidate and the original are then passed through a decision module. The decision module determines a level of similarity between the features of the candidate and the features of the original. The output of the decision module provides an indication of this level of similarity and, depending on this level of similarity, an alert may be generated. A report module may be included to provide an explanation regarding the level of similarity.

Abstract: Systems and methods for collecting data from multiple sources. A server receives a data request for desired content from a requestor. The server generates a request for data objects containing that content and displays that request to at least one potential data provider. The at least one data provider provides a data object in response to the request. In some embodiments, the data provider may label the provided data object. In further embodiments, labels may replace sensitive personal information on the data. The data objects may be obtained by the data providers using their personal portable computing devices. In some embodiments, data providers may upload data objects that are not directed to a specific request. These data objects may then be associated with later data requests.

Abstract: The invention provides, in some aspects, methods of optimizing an app for execution on a client device by identifying components of the app used in such execution by a user having a designated role who accesses the app for download via a designated portal. A component can be, for example, a source code file or portion thereof, including, for example, a portion defining a widget or other user interface element. For each component, the method (i) identifies source code files on which execution of that component depends, e.g., in connection with execution of the particular app by the particular user, and (ii) generates a dependency-ordered stack of the source code files for that component. In further accord with those aspects of the invention, the method combines the dependency-ordered stacks into a single such stack and transfers the source code in an order specified by that stack to the client device for loading and execution.

Abstract: Methods of protein production in a linked culture and production bioreactor system are provided. Such methods include a culture bioreactor (N-1 bioreactor) linked to production bioreactor (N bioreactor). More specifically, the methods include (a) culturing cells with a gene that encodes the protein of interest in a continuous perfusion culture bioreactor (N-1 bioreactor); inoculating a continuously stirred tank reactor (CSTR) production bioreactor (N bioreactor) with cells obtained from step (a); and culturing the cells in the CSTR production bioreactor under conditions that allow production of the protein of interest.

Abstract: A cooking system includes a housing and a liner with a bottom heating element and a side circumferential heating element in a plurality of segments electrically connected. A control subsystem includes a selector for the user switchable between slow cook and stove top modes. Both the bottom heating element and the side circumferential heating element are energized and in the stove top mode. Only the side circumferential heating element is energized in the slow cook mode.

Abstract: Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.

Abstract: A cooking system includes a housing and a liner with a bottom heating element and a side circumferential heating element in a plurality of segments electrically connected. A control subsystem includes a selector for the user switchable between slow cook and stove top modes. Both the bottom heating element and the side circumferential heating element are energized and in the stove top mode. Only the side circumferential heating element is energized in the slow cook mode.

Abstract: A siphoning operation is controlled to meet a target flow rate of liquid being siphoned into a container having a headspace to which a vacuum is induced with an ejector. The incremental mass of liquid siphoned into the container is monitored and the vacuum applied to the container is continuously adjusted. A vacuum regulator responsive to an electric current-to-pressure converter which is coupled to a control unit is provided to control the flow of pressurized fluid delivered to the ejector.