Abstract: The invention relates to protein ligation technologies, purified or recombinant peptides, methods for making peptides and proteins with covalent bonds including reversible covalent bonds such as reversible intermolecular covalent bonds, and uses thereof. In particular, this invention relates to intermolecular ester bonds, particularly reversible ester bonds between the hydroxyl and amide groups of amino acid side chains present in recombinant chimeric peptides and proteins and the use of such peptides and proteins in protein engineering, for example in the preparation of multimeric protein complexes, including functionalised multimeric protein complexes.

Abstract: The invention generally relates to compositions and methods for treating, detecting and assisting in the diagnosis of Streptococcus pyogenes infection, of rheumatic fever or of poststreptococcal glomerulonephritis (PSGN), and compositions and methods for assessing the propensity for developing rheumatic fever or PSGN in subjects in need thereof. Recombinant polypeptides, including recombinant Streptococcus pyogenes SpnA polypeptides, and compositions comprising such polypeptides, for use in such methods are also provided.

Abstract: The invention relates to protein ligation technologies, purified or recombinant peptides, methods for making peptides and proteins with covalent bonds including reversible covalent bonds such as reversible intermolecular covalent bonds, and uses thereof. In particular, this invention relates to intermolecular ester bonds, particularly reversible ester bonds between the hydroxyl and amide groups of amino acid side chains present in recombinant chimeric peptides and proteins and the use of such peptides and proteins in protein engineering, for example in the preparation of multimeric protein complexes, including functionalised multimeric protein complexes.