Abstract: The present invention relates to a method for determining one or more intrinsic properties of a DNA component from a plurality of measurements obtained over a time period from a cell culture, with each cell comprising the DNA component, wherein the DNA component is involved in transcription of one or more target signals, wherein the plurality of measurements comprises measurements relating to the density of the cell culture over the time period and measurements relating to the amount of the one or more target signals in the cell culture over the time period.

Abstract: In some embodiments, a lifeline comprises an anchor arranged to support a cable and a shuttle arranged to move along a length of the cable. The anchor comprises a body portion and a cable engaging portion. The body portion comprises a thickness. The shuttle comprises a first end and a second end. A cavity extends from the first end to the second end and a slot extends from the first end to the second end. The cable extends through the cavity. A width of the slot is greater than the thickness of the body portion. The width of the slot is less than a cross-sectional size of the cable.

Abstract: A bioreactor includes a rigid-walled vessel for containing a biological medium, the vessel having a lid. The bioreactor further includes a probe passing through an aperture in the lid and having a sensing end inside the vessel and a remote end outside the vessel. The sensing end has plural electrodes for immersion in the biological medium, and the remote end is configured for coupling to external devices and transmission thereto of electrical or electromagnetic measurements made by the electrodes. One of the lid and the probe has one or more resiliently deformable mechanisms and the other of the lid and the probe has one or more respective complementary surfaces.

Abstract: The present disclosure relates to the use of anti-PAD4 autoantibodies as a clinical biomarker for rheumatoid arthritis (RA) treatment. The disclosure further provides an assay to detect anti-PAD4 autoantibodies, assay kits for the detection of anti-PAD4 autoantibodies, as well as computer implemented diagnostic methods.

Abstract: The present invention relates to a method for determining one or more intrinsic properties of a DNA component from a plurality of measurements obtained over a time period from a cell culture, with each cell comprising the DNA component, wherein the DNA component is involved in transcription of one or more target signals, wherein the plurality of measurements comprises measurements relating to the density of the cell culture over the time period and measurements relating to the amount of the one or more target signals in the cell culture over the time period.

Abstract: The present invention relates to a method for determining one or more intrinsic properties of a DNA component from a plurality of measurements obtained over a time period from a cell culture, with each cell comprising the DNA component, wherein the DNA component is involved in transcription of one or more target signals, wherein the plurality of measurements comprises measurements relating to the density of the cell culture over the time period and measurements relating to the amount of the one or more target signals in the cell culture over the time period.

Abstract: The present disclosure relates to the use of anti-PAD4 autoantibodies as a clinical biomarker for rheumatoid arthritis (RA) treatment. The disclosure further provides an assay to detect anti-PAD4 autoantibodies, assay kits for the detection of anti-PAD4 autoantibodies, as well as computer implemented diagnostic methods.

Abstract: A liquid filtration system according to the present invention comprises a bioreactor; a filter configured to filter liquid passing therethrough; a perfusion pump configured to move liquid between the bioreactor and the filter; a liquid line providing fluidic communication between the bioreactor, perfusion pump and filter; and a bleed outlet provided on the liquid line, the bleed outlet configured to provide means to remove liquid selectively from the system under the action of the perfusion pump. With the liquid filtration system according to the present invention, both the filtering and bleed functions may be performed under the action of a single pump, thereby significantly reducing the complexity and cost of the components required.

Abstract: A liquid filtration system comprising a syringe pump comprising a gas chamber and a movable plunger, wherein the gas chamber has an aperture at a first end and the plunger forms a seal within the internal walls of the chamber; a liquid chamber having two openings, the openings positioned at opposite ends of the chamber and the first opening connected to the aperture of the gas chamber; a bioreactor in fluidic communication with the second opening of the liquid chamber; a filter arranged to filter liquid passing between the bioreactor and the liquid chamber, the filter comprising a permeate outlet for removing filtered liquid; wherein, in use, the plunger may be moved in a reciprocating motion causing a corresponding movement of gas which drives liquid alternately between the liquid chamber and the bioreactor such that liquid passes through the filter and filtered liquid may be removed via the permeate outlet.

Abstract: In some embodiments, an anchoring stick comprises a shaft, a handle located at one end of the shaft and a hook located at the other end. The hook defines a cavity. The anchoring stick has a first orientation and a second orientation. A perimeter of the cavity comprises an opening in the first orientation. The cavity is defined by a closed perimeter in the second orientation.

Abstract: In some embodiments, a lifeline comprises an anchor arranged to support a cable and a shuttle arranged to move along a length of the cable. The anchor comprises a body portion and a cable engaging portion. The body portion comprises a thickness. The shuttle comprises a first end and a second end. A cavity extends from the first end to the second end and a slot extends from the first end to the second end. The cable extends through the cavity. A width of the slot is greater than the thickness of the body portion. The width of the slot is less than a cross-sectional size of the cable.

Abstract: The present disclosure relates to the use of anti-PAD4 autoantibodies as a clinical biomarker for rheumatoid arthritis (RA) treatment. The disclosure further provides an assay to detect anti-PAD4 autoantibodies, assay kits for the detection of anti-PAD4 autoantibodies, as well as computer implemented diagnostic methods.

Abstract: In at least one embodiment, a lifeline includes a wire rope and a shock absorber. In some embodiments, the shock absorber includes a tubular body defining a lumen therethrough. The tubular body defines a longitudinal axis including curvature along its length.

Abstract: A liquid filtration system according to the present invention comprises a bioreactor; a filter configured to filter liquid passing therethrough; a perfusion pump configured to move liquid between the bioreactor and the filter; a liquid line providing fluidic communication between the bioreactor, perfusion pump and filter; and a bleed outlet provided on the liquid line, the bleed outlet configured to provide means to remove liquid selectively from the system under the action of the perfusion pump. With the liquid filtration system according to the present invention, both the filtering and bleed functions may be performed under the action of a single pump, thereby significantly reducing the complexity and cost of the components required.

Abstract: The present invention relates to a method for determining one or more intrinsic properties of a DNA component from a plurality of measurements obtained over a time period from a cell culture, with each cell comprising the DNA component, wherein the DNA component is involved in transcription of one or more target signals, wherein the plurality of measurements comprises measurements relating to the density of the cell culture over the time period and measurements relating to the amount of the one or more target signals in the cell culture over the time period.

Abstract: In some embodiments, a davit arm system comprises a davit body arranged to engage a support and an arm supported by the davit body. The arm is moveable along a length of the davit body. In some embodiments, a bearing is oriented between said arm and said davit body. In some embodiments, a bearing a polymer sleeve. In some embodiments, a bearing comprises rollers. In some embodiments, a bearing comprises ball transfer bearings. In some embodiments, a bearing excludes moving parts.

Abstract: A liquid filtration system according to the present invention comprises a syringe pump comprising a gas chamber and a movable plunger, wherein the gas chamber has an aperture at a first end and the plunger forms a seal within the internal walls of the chamber; a liquid chamber having two openings, the openings positioned at opposite ends of the chamber and the first opening connected to the aperture of the gas chamber; a bioreactor in fluidic communication with the second opening of the liquid chamber; a filter arranged to filter liquid passing between the bioreactor and the liquid chamber, the filter comprising a permeate outlet for removing filtered liquid; wherein, in use, the plunger may be moved in a reciprocating motion causing a corresponding movement of gas which drives liquid alternately between the liquid chamber and the bioreactor such that liquid passes through the filter and filtered liquid may be removed via the permeate outlet.

Abstract: In at least one embodiment, a lifeline comprises a wire rope and a shock absorber. In some embodiments, the shock absorber comprises a tubular body defining a lumen therethrough. The tubular body defines a longitudinal axis comprising curvature along its length. The wire rope extends through the lumen of the shock absorber.

Abstract: In some embodiments, a fastener assembly is arranged to engage a supporting member. In some embodiments, the fastener assembly comprises a shaft, a first retaining member and a second retaining member. The first retaining member is arranged to engage the shaft, and is sized to pass through an opening in the supporting member in a first orientation and to abut a second side of the supporting member in a second orientation. The second retaining member is arranged to engage the shaft and to abut the first side of the supporting member.

Abstract: In some embodiments, an anchoring stick comprises a shaft, a handle located at one end of the shaft and a hook located at the other end. The hook defines a cavity. The anchoring stick has a first orientation and a second orientation. A perimeter of the cavity comprises an opening in the first orientation. The cavity is defined by a closed perimeter in the second orientation.