Abstract: A bioreactor includes a rigid-walled vessel for containing a biological medium, the vessel having a lid. The bioreactor further includes a probe passing through an aperture in the lid and having a sensing end inside the vessel and a remote end outside the vessel. The sensing end has plural electrodes for immersion in the biological medium, and the remote end is configured for coupling to external devices and transmission thereto of electrical or electromagnetic measurements made by the electrodes. One of the lid and the probe has one or more resiliently deformable mechanisms and the other of the lid and the probe has one or more respective complementary surfaces.

Abstract: A bioreactor includes a rigid-walled vessel for containing a biological medium, the vessel having a lid. The bioreactor further includes a probe passing through an aperture in the lid and having a sensing end inside the vessel and a remote end outside the vessel. The sensing end has plural electrodes for immersion in the biological medium, and the remote end is configured for coupling to external devices and transmission thereto of electrical or electromagnetic measurements made by the electrodes. One of the lid and the probe has one or more resiliently deformable mechanisms and the other of the lid and the probe has one or more respective complementary surfaces.

Abstract: The present invention relates to a method for determining one or more intrinsic properties of a DNA component from a plurality of measurements obtained over a time period from a cell culture, with each cell comprising the DNA component, wherein the DNA component is involved in transcription of one or more target signals, wherein the plurality of measurements comprises measurements relating to the density of the cell culture over the time period and measurements relating to the amount of the one or more target signals in the cell culture over the time period.

Abstract: A bioreactor includes a rigid-walled vessel for containing a biological medium, the vessel having a lid. The bioreactor further includes a probe passing through an aperture in the lid and having a sensing end inside the vessel and a remote end outside the vessel. The sensing end has plural electrodes for immersion in the biological medium, and the remote end is configured for coupling to external devices and transmission thereto of electrical or electromagnetic measurements made by the electrodes. One of the lid and the probe has one or more resiliently deformable mechanisms and the other of the lid and the probe has one or more respective complementary surfaces.

Abstract: The present invention relates to a method for determining one or more intrinsic properties of a DNA component from a plurality of measurements obtained over a time period from a cell culture, with each cell comprising the DNA component, wherein the DNA component is involved in transcription of one or more target signals, wherein the plurality of measurements comprises measurements relating to the density of the cell culture over the time period and measurements relating to the amount of the one or more target signals in the cell culture over the time period.

Abstract: The present invention relates to a method for determining one or more intrinsic properties of a DNA component from a plurality of measurements obtained over a time period from a cell culture, with each cell comprising the DNA component, wherein the DNA component is involved in transcription of one or more target signals, wherein the plurality of measurements comprises measurements relating to the density of the cell culture over the time period and measurements relating to the amount of the one or more target signals in the cell culture over the time period.

Abstract: A liquid filtration system according to the present invention comprises a bioreactor; a filter configured to filter liquid passing therethrough; a perfusion pump configured to move liquid between the bioreactor and the filter; a liquid line providing fluidic communication between the bioreactor, perfusion pump and filter; and a bleed outlet provided on the liquid line, the bleed outlet configured to provide means to remove liquid selectively from the system under the action of the perfusion pump. With the liquid filtration system according to the present invention, both the filtering and bleed functions may be performed under the action of a single pump, thereby significantly reducing the complexity and cost of the components required.

Abstract: A liquid filtration system comprising a syringe pump comprising a gas chamber and a movable plunger, wherein the gas chamber has an aperture at a first end and the plunger forms a seal within the internal walls of the chamber; a liquid chamber having two openings, the openings positioned at opposite ends of the chamber and the first opening connected to the aperture of the gas chamber; a bioreactor in fluidic communication with the second opening of the liquid chamber; a filter arranged to filter liquid passing between the bioreactor and the liquid chamber, the filter comprising a permeate outlet for removing filtered liquid; wherein, in use, the plunger may be moved in a reciprocating motion causing a corresponding movement of gas which drives liquid alternately between the liquid chamber and the bioreactor such that liquid passes through the filter and filtered liquid may be removed via the permeate outlet.

Abstract: A liquid filtration system according to the present invention comprises a bioreactor; a filter configured to filter liquid passing therethrough; a perfusion pump configured to move liquid between the bioreactor and the filter; a liquid line providing fluidic communication between the bioreactor, perfusion pump and filter; and a bleed outlet provided on the liquid line, the bleed outlet configured to provide means to remove liquid selectively from the system under the action of the perfusion pump. With the liquid filtration system according to the present invention, both the filtering and bleed functions may be performed under the action of a single pump, thereby significantly reducing the complexity and cost of the components required.

Abstract: The present invention relates to a method for determining one or more intrinsic properties of a DNA component from a plurality of measurements obtained over a time period from a cell culture, with each cell comprising the DNA component, wherein the DNA component is involved in transcription of one or more target signals, wherein the plurality of measurements comprises measurements relating to the density of the cell culture over the time period and measurements relating to the amount of the one or more target signals in the cell culture over the time period.

Abstract: A liquid filtration system according to the present invention comprises a syringe pump comprising a gas chamber and a movable plunger, wherein the gas chamber has an aperture at a first end and the plunger forms a seal within the internal walls of the chamber; a liquid chamber having two openings, the openings positioned at opposite ends of the chamber and the first opening connected to the aperture of the gas chamber; a bioreactor in fluidic communication with the second opening of the liquid chamber; a filter arranged to filter liquid passing between the bioreactor and the liquid chamber, the filter comprising a permeate outlet for removing filtered liquid; wherein, in use, the plunger may be moved in a reciprocating motion causing a corresponding movement of gas which drives liquid alternately between the liquid chamber and the bioreactor such that liquid passes through the filter and filtered liquid may be removed via the permeate outlet.

Abstract: Couplers for attaching an accessory to an excavator arm of an excavator. Couplers having a first side for attaching the coupler to the excavator arm and a second side onto which the accessory will be coupled. The coupler includes a latch for selectively securing and releasing an attachment pin of the accessory in a jaw, groove, hook or slot in the second side of the coupler. The coupler is fully controllable from within the cab of the excavator and it allows improved security in the securement of the accessory to the coupler, i.e. preventing accidental decouplings, but while still allowing intentional decoupling operations to be carried out without undue burden.

Abstract: Couplers for attaching an accessory to an excavator arm of an excavator. Couplers having a first side for attaching the coupler to the excavator arm and a second side onto which the accessory will be coupled. The coupler includes a latch for selectively securing and releasing an attachment pin of the accessory in a jaw, groove, hook or slot in the second side of the coupler. The coupler is fully controllable from within the cab of the excavator and it allows improved security in the securement of the accessory to the coupler, i.e. preventing accidental decouplings, but while still allowing intentional decoupling operations to be carried out without undue burden.

Abstract: Couplers for attaching an accessory to an excavator arm of an excavator. Couplers having a first side for attaching the coupler to the excavator arm and a second side onto which the accessory will be coupled. The coupler includes a latch for selectively securing and releasing an attachment pin of the accessory in a jaw, groove, hook or slot in the second side of the coupler. The coupler is fully controllable from within the cab of the excavator and it allows improved security in the securement of the accessory to the coupler, i.e. preventing accidental decouplings, but while still allowing intentional decoupling operations to be carried out without undue burden.

Abstract: Couplers for attaching an accessory to an excavator arm of an excavator. Couplers having a first side for attaching the coupler to the excavator arm and a second side onto which the accessory will be coupled. The coupler includes a latch for selectively securing and releasing an attachment pin of the accessory in a jaw, groove, hook or slot in the second side of the coupler. The coupler is fully controllable from within the cab of the excavator and it allows improved security in the securement of the accessory to the coupler, i.e. preventing accidental decouplings, but while still allowing intentional decoupling operations to be carried out without undue burden.

Abstract: Couplers for attaching an accessory to an excavator arm of an excavator. Couplers having a first side for attaching the coupler to the excavator arm and a second side onto which the accessory will be coupled. The coupler includes a latch for selectively securing and releasing an attachment pin of the accessory in a jaw, groove, hook or slot in the second side of the coupler. The coupler is fully controllable from within the cab of the excavator and it allows improved security in the securement of the accessory to the coupler, i.e. preventing accidental decouplings, but while still allowing intentional decoupling operations to be carried out without undue burden.

Abstract: Couplers for attaching an accessory to an excavator arm of an excavator. Couplers having a first side for attaching the coupler to the excavator arm and a second side onto which the accessory will be coupled. The coupler includes a latch for selectively securing and releasing an attachment pin of the accessory in a jaw, groove, hook or slot in the second side of the coupler. The coupler is fully controllable from within the cab of the excavator and it allows improved security in the securement of the accessory to the coupler, i.e. preventing accidental decouplings, but while still allowing intentional decoupling operations to be carried out without undue burden.

Abstract: A pipetting device has top and bottom supports, the first support supporting a pipetting head and the second support defining a location for one or more vessels or substrates to or from which fluid is to be pipetted in use. The pipetting head has a two-dimensional array of pipetting cylinders having pistons for controlling the aspirating or dispensing of liquid, each pipetting cylinder piston being connected to a platen for simultaneous movement of the pistons within the cylinders. The pipetting head and the second support are relatively movable to one another between an operating and a retracted relationship. A detachable pipetting tip holder has a two-dimensional array of pipetting tips which are connectable to the pipetting cylinders for aspirating liquid from or dispensing liquid into or onto a corresponding vessel.

Abstract: In the formation of an envelope or jacket for a disc, comprising laminating a nonwoven fabric to a plastic sheet, superposing two such laminates with the nonwoven fabrics facing each other, and joining such superposed laminates along all edges but one, thereby to form an envelope, the improvement which comprises effecting the laminating by providing a fusible layer between the nonwoven fabric and plastic sheet and heating to soften the fusible layer while pressing to join the nonwoven fabric to the plastic. The low melting fusible layer eliminates the tendency, in its absence, to pull particles of the jacket plastic through the nonwoven liner layer where it could damage the object to be encased in the envelope. Advantageously the fusible layer is a spray spun low melting polyester which, in addition to polyvinylchloride, is bondable to polycarbonate.