Patents by Inventor Paul Hardenbol

Paul Hardenbol has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • USE OF EDITING CASSETTES FOR TARGETED SEQUENCING
    Publication number: 20210246441
    Abstract: The present disclosure relates to methods and compositions to enable use of editing cassettes for nucleic-acid guided editing and then repurposing the cassettes to increase targeted sequencing efficiency and reduce sequencing costs of the edited genomes.
    Type: Application
    Filed: February 3, 2021
    Publication date: August 12, 2021
    Inventors: Paul Hardenbol, Kara Juneau, Michael Graige
  • Increased nucleic-acid guided cell editing in yeast
    Patent number: 11066675
    Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.
    Type: Grant
    Filed: January 2, 2021
    Date of Patent: July 20, 2021
    Assignee: Inscripta, Inc.
    Inventors: Krishna Kannan, Miles Gander, Eileen Spindler, Paul Hardenbol
  • Methods and systems for processing polynucleotides
    Patent number: 11035002
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: August 20, 2020
    Date of Patent: June 15, 2021
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol
  • Methods and systems for processing polynucleotides
    Patent number: 11021749
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: August 20, 2020
    Date of Patent: June 1, 2021
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Kevin Ness, Serge Saxonov, Paul Hardenbol
  • NANOPORE-BASED NUCLEIC ACID ANALYSIS WITH MIXED FRET DETECTION
    Publication number: 20210130887
    Abstract: Various methods, systems and devices for optical detection and analysis of polymers, such as polynucleotides, using nanopores, e.g., for determining sequences of nucleic acids, are provided herein. In certain variations, methods and systems for determining a nucleotide sequence of a polynucleotide, which include measuring mixed FRET signals as a polynucleotide translocates through a nanopore and determining a nucleotide sequence of the polynucleotide from the mixed FRET signals, are provided.
    Type: Application
    Filed: August 6, 2020
    Publication date: May 6, 2021
    Applicant: Quantapore, Inc.
    Inventors: Martin HUBER, Bason E. CLANCY, Paul HARDENBOL
  • INCREASED NUCLEIC-ACID GUIDED CELL EDITING IN YEAST
    Publication number: 20210130834
    Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.
    Type: Application
    Filed: January 2, 2021
    Publication date: May 6, 2021
    Inventors: Krishna Kannan, Miles Gander, Eileen Spindler, Paul Hardenbol
  • METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES
    Publication number: 20210079463
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Application
    Filed: April 20, 2020
    Publication date: March 18, 2021
    Inventors: Benjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Michael Schnall-Levin, Mirna Jarosz
  • Increased nucleic-acid guided cell editing in yeast
    Patent number: 10927385
    Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.
    Type: Grant
    Filed: June 17, 2020
    Date of Patent: February 23, 2021
    Assignee: Inscripta, Inc.
    Inventors: Krishna Kannan, Miles Gander, Eileen Spindler, Paul Hardenbol
  • METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES
    Publication number: 20210002721
    Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
    Type: Application
    Filed: April 9, 2020
    Publication date: January 7, 2021
    Inventors: Christopher Hindson, Michael Schnall-Levin, Andrew Price, Paul Hardenbol, Yuan Li
  • INCREASED NUCLEIC-ACID GUIDED CELL EDITING IN YEAST
    Publication number: 20200407732
    Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.
    Type: Application
    Filed: June 17, 2020
    Publication date: December 31, 2020
    Inventors: Krishna Kannan, Miles Gander, Eileen Spindler, Paul Hardenbol
  • METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES
    Publication number: 20200385805
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Application
    Filed: August 20, 2020
    Publication date: December 10, 2020
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol, Rajiv Bharadwaj, Xinying Zheng, Phillip Belgrader
  • METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES
    Publication number: 20200377942
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Application
    Filed: August 20, 2020
    Publication date: December 3, 2020
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol, Rajiv Bharadwaj, Xinying Zheng, Phillip Belgrader
  • METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES
    Publication number: 20200291472
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Application
    Filed: January 23, 2020
    Publication date: September 17, 2020
    Inventors: Benjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol
  • Methods and systems for processing polynucleotides
    Patent number: 10760124
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: December 5, 2017
    Date of Patent: September 1, 2020
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol, Rajiv Bharadwaj, Xinying Zheng, Phillip Belgrader
  • Methods and systems for processing polynucleotides
    Patent number: 10752950
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: June 7, 2019
    Date of Patent: August 25, 2020
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol
  • Methods and systems for processing polynucleotides
    Patent number: 10752949
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: December 6, 2018
    Date of Patent: August 25, 2020
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol
  • Methods and Systems for Processing Polynucleotides
    Publication number: 20200232027
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing. In some cases, this disclosure provides methods for the generation of polynucleotide barcode libraries, and for the attachment of such polynucleotides to target polynucleotides.
    Type: Application
    Filed: November 27, 2019
    Publication date: July 23, 2020
    Inventors: Benjamin Hindson, Mirna Jarosz, Paul Hardenbol, Michael Schnall-Levin, Kevin Ness, Serge Saxonov
  • Methods and Systems for Processing Polynucleotides
    Publication number: 20200199669
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Application
    Filed: September 13, 2019
    Publication date: June 25, 2020
    Inventors: Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov, Paul Hardenbol, Rajiv Bharadwaj, Xinying Zheng, Phillip Belgrader
  • PROCESSES AND SYSTEMS FOR PREPARATION OF NUCLEIC ACID SEQUENCING LIBRARIES AND LIBRARIES PREPARED USING SAME
    Publication number: 20200190551
    Abstract: This disclosure provides methods for preparing a sequencing library including the steps of providing a template nucleic acid sequence, dNTPs, dUTP, a primer, a polymerase, a dUTP excising enzyme, and a plurality of beads including oligonucleotide adapter sequence segments; amplifying the template nucleic acid with the polymerase, dNTPs, dUTP and random hexamer to provide a complementary nucleic acid sequence including occasional dUTPs; and excising the incorporated dUTPs with the dUTP excising enzyme to provide nicks in the complementary nucleic acid sequence to provide a sequencing library.
    Type: Application
    Filed: December 17, 2019
    Publication date: June 18, 2020
    Inventors: Paul Hardenbol, Pranav Patel, Benjamin Hindson, Paul William Wyatt, Keith Bjornson, Indira Wu, Zahra Kamila Belhocine
  • Methods and systems for processing polynucleotides
    Patent number: 10676789
    Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
    Type: Grant
    Filed: December 21, 2017
    Date of Patent: June 9, 2020
    Assignee: 10X GENOMICS, INC.
    Inventors: Benjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Michael Schnall-Levin, Mirna Jarosz