Abstract: A chill assembly for chilling a molten material during formation of a part is disclosed. The chill assembly includes an upper platform and a mold platform for receiving a mold package to be filled with the molten material to form the part. The upper platform has a first platen moveable in a vertical direction relative to the molding platform. A plurality of chills are moveably supported by the first platen for quenching the molten material. Each of the chills are moveable between a pre-chill position and a post-chill position. An alignment sub-assembly engages the chills and aligns the chills in the pre-chill position. The alignment sub-assembly re-aligns the chills after the chills have quenched the molten material and moved to the post-chill position. The alignment sub-assembly is capable of precisely aligning and re-aligning the chills after each successive quenching of the molten material.

Abstract: A chill assembly for chilling a molten material during formation of a part is disclosed. The chill assembly includes an upper platform and a mold platform for receiving a mold package to be filled with the molten material to form the part. The upper platform has a first platen moveable in a vertical direction relative to the molding platform. A plurality of chills are moveably supported by the first platen for quenching the molten material. Each of the chills are moveable between a pre-chill position and a post-chill position. An alignment sub-assembly engages the chills and aligns the chills in the pre-chill position. The alignment sub-assembly re-aligns the chills after the chills have quenched the molten material and moved to the post-chill position. The alignment sub-assembly is capable of precisely aligning and re-aligning the chills after each successive quenching of the molten material.

Abstract: The present invention provides materials and methods for introducing genetic disruptions in bacterial genetic material, especially in that of Streptomyces spp.. A novel transposon is provided, which has an origin of transfer between inverted repeat sequences. The transposon may also include a genetic marker. The transposon introduces a disruption into DNA of interest, which disruption may then be conjugated into host bacteria, including bacteria of other species or strains. The host bacteria is incubated at conditions suitable for homologous recombination between the conjugated DNA and the host DNA. The effect of the disruption in different genetic backgrounds can therefore be investigated. The disruption may be stored as a mobile genetic element ready for transfer to a test host.