Abstract: Cross-linked polymeric gels or films are prepared by dissolving in a suitable solvent a linear polymer or polymers at least one of which is predominantly (at least 80%) made from monomers containing pendant amidocarbonyl or oxycarbonyl groups. For the cross-linking reaction are present either hydroxyl groups, present as either pendant groups from the polymer, or as low molecular weight polyols; or amino groups, present as either pendant groups from the polymer, or as low molecular weight diamines or polyamines. Either no catalyst is present or a strongly acidic catalyst (which may also be a pendant group on a polymer) is used. The solvent is removed by coagulation and/or drying and the dry material is heat-cured, thus cross-linking and insolubilizing the polymer or polymers.

Abstract: A membrane is produced by dissolving, in a water-miscible solvent, a water insoluble copolymer of acrylontrile with at least one monomer selected from the group consisting of an aminostyrene, a vinyl pyridine and an N-hydroxy-containing-substituent-acrylamide, casting said solution to form a thin layer of solution, contacting said solution with water thereby to coagulate the copolymer into a film, and washing away from the copolymer film the solution of solvent and water. The amine or hydroxy group of the copolymer is then activated and coupled with a ligand such as glucose isomerase, chymotrypsin or Protein A. The coupled membranes can then be used for biological separations and reactions.

Abstract: Cross-linked polymeric gels or films are prepared by dissolving in a suitable solvent a linear polymer or polymers containing pendant amidocarbonyl or oxycarbonyl groups, hydroxyl groups present as either pendant groups from the polymer or as a low molecular weight polyol, and a strongly acidic catalyst (which may also be a pendant group on a polymer). The solvent is removed by coagulation and/or drying and the dry material is heat-cured, thus cross-linking and insolubilizing the polymer or polymers.