Abstract: A process for maintaining a high rate of mass transfer of nutrients contained in a liquid feed stream to a biofilm of microorganisms supported on a bed of particulate matter. The microorganisms in the biofilm are subjected to a continuous, uniform flow of the liquid feed steam and a countercurrent flow of respiratory air so as to support biofilm growth. The bed is periodically pulsed with air at a pressure sufficient to shed microorganisms from the biofilm, and at intervals sufficient to avoid preferential channeling of the liquid feed steam through the bed and/or to disrupt and rearrange the bed of particulate matter.