Abstract: The invention relates to the field of medicine and microbiology, more specifically to means and methods for the treatment of infections caused by Gram-negative pathogens, in particular those showing or being prone to developing drug resistance. Provided is an admixture of (i) an inner membrane acting compound having membrane-permeating activity and/or lipid H binding activity; and (ii) one or more antimicrobial peptide(s) selected from the group consisting of RRLFRRIRWL-NH2 (GNP-6); GNNRPVYIPQPRPPHPRL (GNP-1); RIWVIWRR—NH2 (GNP-5); GIGKHVGKALKGLKGLLKGLGEC (GNP-7); and Xi X2IVQRIKKWLX3-NH2, wherein Xi is absent or K; X2 is R, K or A; and X3 is absent or R; wherein said one or more antimicrobial peptide(s) may comprise or consist of D- or L-amino acids.

Abstract: The present invention provides proteins/genes, which are essential for survival, and consequently, for virulence of Streptococcus pneumoniae in vivo, and thus are ideal vaccine candidates for a vaccine preparation against pneumococcal infection. Further, also antibodies against said protein(s) are included in the invention.

Abstract: The present invention provides proteins/genes, which are essential for survival, and consequently, for virulence of Streptococcus pneumoniae in vivo, and thus are ideal vaccine candidates for a vaccine preparation against pneumococcal infection. Further, also antibodies against said protein(s) are included in the invention.

Abstract: A method for applying an image onto a receiving medium uses an ultraviolet curable phase change ink. The method includes the steps of pre-curing the ultraviolet curable ink and post-curing the ultraviolet curable ink. An ink jet apparatus is configured to perform the above mentioned method.

Abstract: A method for applying an image onto a receiving medium uses an ultraviolet curable phase change ink. The method includes the steps of pre-curing the ultraviolet curable ink and post-curing the ultraviolet curable ink. An ink jet apparatus is configured to perform the above mentioned method.

Abstract: A method for printing an image on an image receiving member is provided, in which a print head having an array of print elements moves in a scanning direction to print image lines and the image receiving member is advanced in a transport direction after printing a swath. In order to obtain various contiguous swaths of print lines without a light or dark line between the swaths, the position of a prior swath printed preceding to a current swath is determined during the printing of the current swath. A digital mask is applied to the print data that is associated with the print elements at the to the prior swath adjoining edge of the current swath in dependence on the determination of the position of the prior swath.

Abstract: The invention relates to a method for operating an inkjet device, the inkjet device comprising a piezo-electric element. The inkjet device may be configured to operate in a plurality of modes, the plurality of modes comprising a first mode, wherein the inkjet device is in an off state; a second mode, wherein the inkjet device is in a standby state; and a third mode, wherein the inkjet device is in an operative state. Depending on the state of the inkjet device, the BIAS voltage applied to the piezo-electric element is selected.

Abstract: The invention relates to the production of heterologous polypeptides in a recombinant host cell. More specifically, it relates to the production and secretion of peptides, such as biologically active peptides, that are modified by one or more lantibiotic-synthesizing enzymes. Provided is a nucleic acid construct encoding a polypeptide comprising 1) a non-lantibiotic export signal that is recognized by a non-lantibiotic export system; 2) a lantibiotic leader peptide that is recognized by at least a lantibiotic dehydratase such as LanB and, C-terminally of said export signal and said leader peptide, 3) a peptide of interest containing one or more serine or threonine residue(s) which can be posttranslationally dehydrated by said dehydratase.

Abstract: A method of printing an image on a receiving medium includes increasing the number of pixels in each row of a part of the image by x pixels, resulting in the part of the image including m by n+x pixels; assigning a printing element to each added pixel; identifying pixels to which a compensating printing element of the defective printing element is assigned; changing the value of at least one identified pixel into an integer value greater than zero; increasing the first printer resolution in the main-scanning direction to a second printer resolution by multiplying the first printer resolution in the main-scanning direction with a factor equal to (n+x)/n; and printing the part of the image according to the second printer resolution in the main-scanning direction and according to the values of the pixels of the part of the image.

Abstract: A method for printing an image on an image receiving member is provided, in which a print head having an array of print elements moves in a scanning direction to print image lines and the image receiving member is advanced in a transport direction after printing a swath. In order to obtain various contiguous swaths of print lines without a light or dark line between the swaths, the position of a prior swath printed preceding to a current swath is determined during the printing of the current swath. A digital mask is applied to the print data that is associated with the print elements at the to the prior swath adjoining edge of the current swath in dependence on the determination of the position of the prior swath.

Abstract: A method of printing an image by a printer having an asymmetric printer resolution includes multi-level halftoning the image by assigning a level out of a number of levels to each pixel of the image. The method further includes determining the number of levels on the basis of the asymmetric printer resolution, selecting for each level a collection of at least one binary pattern, each binary pattern consisting of a plurality of sub-pixels the number of which is determined by the number of levels, selecting for each pixel of the halftoned image a binary pattern from the collection selected for the level assigned to the pixel, transforming each pixel of the halftoned image into the selected binary pattern, and printing the binary patterns of the transformed pixels using the asymmetric printer resolution. A printer is configured to perform the method.

Abstract: The present invention provides proteins/genes, which are essential for survival, and consequently, for virulence of Streptococcus pneumoniae in vivo, and thus are ideal vaccine candidates for a vaccine preparation against pneumococcal infection. Further, also antibodies against said protein(s) are included in the invention.

Abstract: Methods for improving binding of a proteinaceous substance to cell-wall material of a Gram-positive bacterium are disclosed. The proteinaceous substance includes an AcmA cell-wall binding domain, homolog or functional derivative thereof. The method includes treating the cell-wall material with a solution capable of removing a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from the cell-wall material and contacting the proteinaceous substance with the cell-wall material.

Abstract: The present invention provides proteins/genes, which are essential for survival, and consequently, for virulence of Streptococcus pneumoniae in vivo, and thus are ideal vaccine candidates for a vaccine preparation against pneumococcal infection. Further, also antibodies against said protein(s) are included in the invention.

Abstract: Methods for improving binding of a proteinaceous substance to cell-wall material of a Gram-positive bacterium are disclosed. The proteinaceous substance includes an AcmA cell-wall binding domain, homolog or functional derivative thereof. The method includes treating the cell-wall material with a solution capable of removing a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from the cell-wall material and contacting the proteinaceous substance with the cell-wall material.

Abstract: The invention relates to a promoter DNA sequence highly suited in an improved expression cloning method for isolation of DNA sequences comprising a DNA sequence encoding a protein of interest in a host cell and to the improved expression cloning method wherein use is made of this promoter. The isolated DNA sequences are useful in processes for producing a protein of interest.

Abstract: Methods for improving binding of a proteinaceous substance to cell-wall material of a Gram-positive bacterium are disclosed. The proteinaceous substance includes an AcmA cell-wall binding domain, homolog or functional derivative thereof. The method includes treating the cell-wall material with a solution capable of removing a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from the cell-wall material and contacting the proteinaceous substance with the cell-wall material.

Abstract: The invention relates to the production of heterologous polypeptides in a recombinant host cell. More specifically, it relates to the production and secretion of peptides, such as biologically active peptides, that are modified by one or more lantibiotic-synthesizing enzymes. Provided is a nucleic acid construct encoding a polypeptide comprising 1) a non-lantibiotic export signal that is recognized by a non-lantibiotic export system; 2) a lantibiotic leader peptide that is recognized by at least a lantibiotic dehydratase such as LanB and, C-terminally of said export signal and said leader peptide, 3) a peptide of interest containing one or more serine or threonine residue(s) which can be posttranslationally dehydrated by said dehydratase.

Abstract: A method for generating a dither mask is disclosed using a local stochastic technique for use for the halftoning of grey scale images by a pixel by pixel comparison of the images against the dither mask. The dither mask is generated by producing a sequence of dot profiles (each dot profile of the sequence corresponding to a grey scale level) using a stacking constraint with incremental grey scale level such that for each dot profile of the sequence the same pixels are selected compared to the preceding dot profile and the increment in the or each dot profile is realized by selecting at least one additional pixel. When determining the position of an additional pixel, the aspect ratio between the dot pitch in a predetermined direction and a direction perpendicular thereto when rendering images is taken into account.

Abstract: A system and method are provided for processing digital multi-colour images for the reproduction thereof on a colour printing and/or copying system. Image dots of marking particles of the respective colours associated with the respective colour separation images are positioned contiguous to each other as long as the print area corresponding to the image part to be reproduced is not fully covered so as to create a first layer of marking particles. If the area is fully covered and the image is not yet completely rendered, a second and any following layer of contiguously positioned marking particles is formed on top of the first layer of image dots. The halftoning of the digital image is such that within the same layer all images dots, irrespective of their colour, are obtained using a raster of the same matrix structure, while image dots associated with different layers are obtained using rasters of different matrix structures.