Abstract: Anti-peptide antibodies (APAs) are extremely important tools for biomedical research. Many important techniques, such as immunoblots, ELISA immunoassays, immunocytochemistry, and protein microarrays are intrinsically linked to APA function and completely dependent on APA quality. Unfortunately, not all commercially-available APAs have good antigen binding characteristics; as a result, researchers are often unable to perform high quality protein analysis experiments. This disclosure describes a new method for the scalable production of polyclonal APAs using recombinant antigens. These recombinant peptide antigens have several advantages over traditional peptide antigens which improve the ease and speed of antibody production. The recombinant antigens can be scalably produced and purified much faster than traditional synthetic peptide-conjugates.

Abstract: Anti-peptide antibodies (APAs) are extremely important tools for biomedical research. Many important techniques, such as immunoblots, ELISA immunoassays, immunocytochemistry, and protein microarrays are intrinsically linked to APA function and completely dependent on APA quality. Unfortunately, not all commercially-available APAs have good antigen binding characteristics; as a result, researchers are often unable to perform high quality protein analysis experiments. This disclosure describes a new method for the scalable production of polyclonal APAs using recombinant antigens. These recombinant peptide antigens have several advantages over traditional peptide antigens which improve the ease and speed of antibody production. The recombinant antigens can be scalably produced and purified much faster than traditional synthetic peptide-conjugates.

Abstract: Anti-peptide antibodies (APAs) are extremely important tools for biomedical research. Many important techniques, such as immunoblots, ELISA immunoassays, immunocytochemistry, and protein microarrays are intrinsically linked to APA function and completely dependent on APA quality. Unfortunately, not all commercially-available APAs have good antigen binding characteristics; as a result, researchers are often unable to perform high quality protein analysis experiments. This disclosure describes a new method for the scalable production of polyclonal APAs using recombinant antigens. These recombinant peptide antigens have several advantages over traditional peptide antigens which improve the ease and speed of antibody production. The recombinant antigens can be scalably produced and purified much faster than traditional synthetic peptide-conjugates.

Abstract: Provided is a sensitive method to specifically detect nucleic acid sequences using a combination of DNA replication based signal amplification (e.g., PCR) and lateral flow analyte detection. The disclosed method uses a dual-labeled DNA probe complementary to a region of the target DNA sequence. Cleavage of the probe, caused by the presence of the amplified DNA target, is detected using lateral flow methods.

Abstract: Anti-peptide antibodies (APAs) are extremely important tools for biomedical research. Many important techniques, such as immunoblots, ELISA immunoassays, immunocytochemistry, and protein microarrays are intrinsically linked to APA function and completely dependent on APA quality. Unfortunately, not all commercially-available APAs have good antigen binding characteristics; as a result, researchers are often unable to perform high quality protein analysis experiments. This disclosure describes a new method for the scalable production of polyclonal APAs using recombinant antigens. These recombinant peptide antigens have several advantages over traditional peptide antigens which improve the ease and speed of antibody production. The recombinant antigens can be scalably produced and purified much faster than traditional synthetic peptide-conjugates.

Abstract: A retractor assembly suitable for hip or other joint surgery comprising a structural member (1), which consists of a handle (2) that extends proximal through a curved region (4) to a distal region (3) adapted for use in a suitable patient, a light ducting member (5), which is at least in part in substantial conformity to the curved region (4), adapted in use to receive light and reliant on internal reflection of the light, to transmit such received light about at least part of the curved region to an emission outlet (7) which casts light to a zone at and/or about said distal region (3), and a shield (6) overlying at least that part of the light ducting means (5) in substantial conformity to at least part of the curved region (4), wherein the light ducting member (5), the shield (6) and the structural member (1) can be brought into assembly so that directly and/or indirectly each of the light ducting member (5) and the shield (6) is in a supported condition relative to the structural member (1).

Abstract: A browser-enabled system of software and services that focuses on connecting people across spatial, temporal, and organizational barriers to achieve specific objectives. The system includes a client server system that provides a virtual meeting place for a learning community and a structure through which that community can achieve its goals.

Abstract: A method of preparing an amine-free emulsion of alkaline earth metal sulfonates is disclosed. A solution of water and a nonionic, associative thickener of the type normally used for viscosity and thixotropy modification of latex compounds is combined with an overbased or neutral earth metal sulfonate or sulfonate complex to produce the emulsion. Mixtures of these emulsified products with other ingredients such as neutral diluent oils, linear alcohol/hydrocarbon mixtures, tall oil fatty acids, non-ionic surfactants, oxidized polyethylene waxes, small levels of microcrystalline wax, petrolatums, and/or calcium salts of oxidized petrolatums is also disclosed. The resulting emulsions find application in the modification of asphalt emulsions, the formulation of temporary coatings for the protection of painted automotive surfaces (transit coatings for automobiles) and in the prevention of corrosion of metal surfaces.

Abstract: A browser-enabled system of software and services that focuses on connecting people across spatial, temporal, and organizational barriers to achieve specific objectives. The system includes a client server system that provides a virtual meeting place for a learning community and a structure through which that community can achieve its goals.

Abstract: An intermediate for a mailer type business form is provided that is Z-folded to produce a mailer type business form with built-in reply envelope. A large area is provided for printing statement, remittance and/or other information and yet the reply envelope produced accepts a conventional size personal check without folding. This is accomplished by providing a re-wettable adhesive for forming and sealing the reply envelope. The outgoing address indicia is imaged on the top face of one of the panels that forms the return envelope so that the outgoing address is concealed when the reply envelope is formed. The reply address indicia is imaged on the bottom face on the second panel defining the reply envelope so that when the reply envelope is formed, the reply address is the only exposed address on the reply envelope.