Abstract: A protein expression vector that expresses large quantities of recombinant proteins under anoxic or microaerobic conditions by inducing expression with nitrate. The vector backbone is pUC19 and protein expression is driven by the E. coli flavohemoglobin promoter, which is inducible by nitrate, nitrite, or nitric oxide under conditions of low oxygen. The Nde1 site of pUC19 has been destroyed by filling in with Klenow fragment and religating the vector. An Nde1 site in the promoter provides an in-frame start methionine and a standard polylinker is available for ease of subcloning. The vector is named pANX for Plasmid ANaerobic eXpression.

Abstract: A fuel feed and fuel vaporization system for an internal combustion engine comprising a closed circuit feed line and a separate intake for air and for fuel. Each intake having a preset and predetermined and adjustable flow meter regulator valve connected to a transducer and accelerator lever for controlling the flow of air and fuel according to the size and capacity of the engine. The air and fuel are electrically heated by a series of resistive elements inside separate heat exchange units located inside the intake manifold and casing before mixing in a common heat exchange and being fed though a series of electrically operated valves into the combustion chamber of the engine. No carburetor or fuel injection system is needed. A thermostat or time delay controls the temperature to the heat exchange.