Abstract: The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

Abstract: A method is provided for authenticating a botanical. The method includes identifying at least one marker compound of the botanical. A sample comprising the botanical is injected into a chromatography system that includes a mobile phase delivery module, an autosampler, a chromatography column, a chromatography column manager, and at least one detector. The method also includes extracting a chromatogram of the at least one marker compound and extracting a spectrum at a chromatographic peak retention time of the at least one marker compound. The method also includes quantifying at least one peak of the extracted chromatogram and quantifying at least one band of the extracted spectrum. The quantified at least one peak of the extracted chromatogram and the quantified at least one band of the extracted spectrum are compared to a set of authentication criteria to determine authenticity of the botanical.

Abstract: The present disclosure discusses a method of separating a sample (e.g., small organic acid metabolite) including coating a flow path of a chromatographic system. The coating along the flow path is vapor deposited and prevents or severely decreases metal interactions between the metallic chromatographic system and the sample.

Abstract: A device for separating analytes is disclosed. The device has a sample injector, sample injection needle, sample reservoir container in communication with the sample injector, chromatography column downstream of the sample injector, and fluid conduits connecting the sample injector and the column. The interior surfaces of the fluid conduits, sample injector, sample reservoir container, and column form a flow path having wetted surfaces. A portion of the wetted surfaces of the flow path are coated with an alkylsilyl coating that is inert to at least one of the analytes. The alkylsilyl coating has the Formula I: R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20, -(C1-C10)[NH(CO)NH(C1-C10)]1-20-, or -(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20-.

Abstract: The present disclosure discusses a method of separating a sample (e.g., small organic acid metabolite) including coating a flow path of a chromatographic system. The coating along the flow path is vapor deposited and prevents or severely decreases metal interactions between the metallic chromatographic system and the sample.

Abstract: The present invention provides the use of charged surface reversed phase chromatographic materials along with standard reversed-phase LC and mass spectrometry compatible conditions for the retention, separation, purification, and characterization of acidic, polar molecules, including, but not limited to, organic acids, ?-amino acids, phosphate sugars, nucleotides, other acidic, polar biologically relevant molecules. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier.

Abstract: The present disclosure provides methods for the separation and quantitation of enantiomers of vitamin E using supercritical fluid chromatography (SFC) or carbon dioxide-based chromatography on chiral columns. The disclosed methods may be used to quantitatively determine the concentration of RRR-?-tocopherol in foods, food ingredients, dietary supplements, vitamin premixes, nutritional formulas, and medicines. Further provided is a method of differentiating the source of ?-tocopherol as natural or synthetic.

Abstract: The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

Abstract: The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

Abstract: The present disclosure discusses a method of separating a sample (e.g., B vitamins and their vitamers) including coating a flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating (e.g., organosilica coating) of the flow path. The applied coating provides an inert barrier that is beneficial for B vitamin analysis, including improved peak shape (less tailing and narrower peak), high sensitivity, and no carry-over.

Abstract: The present disclosure provides methods for the separation and quantitation of enantiomers of vitamin E using supercritical fluid chromatography (SFC) or carbon dioxide-based chromatography on chiral columns. The disclosed methods may be used to quantitatively determine the concentration of RRR-?-tocopherol in foods, food ingredients, dietary supplements, vitamin premixes, nutritional formulas, and medicines. Further provided is a method of differentiating the source of ?-tocopherol as natural or synthetic.

Abstract: The present disclosure discusses a method of separating a sample (e.g., small organic acid metabolite) including coating a flow path of a chromatographic system. The coating along the flow path is vapor deposited and prevents or severely decreases metal interactions between the metallic chromatographic system and the sample.

Abstract: The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

Abstract: The present disclosure relates to methodologies, systems, and devices for screening lipids. The technique includes selecting a set of standards to identify at least one desired class of lipids, spiking the standards into a biological sample to form a sample matrix, extracting the lipids from the sample matrix, and introducing the sample matrix into a chromatography system to separate the desired class of lipids. Once the lipids are separated into different lipid classes using HILIC chromatography, they are directed to a detector, and the separated lipids are quantified based on a comparison between the measured detector response and a calibration curve generated with a known concentration of the selected set of standards.

Abstract: A method is provided for authenticating a botanical. The method includes identifying at least one marker compound of the botanical. A sample comprising the botanical is injected into a chromatography system that includes a mobile phase delivery module, an autosampler, a chromatography column, a chromatography column manager, and at least one detector. The method also includes extracting a chromatogram of the at least one marker compound and extracting a spectrum at a chromatographic peak retention time of the at least one marker compound. The method also includes quantifying at least one peak of the extracted chromatogram and quantifying at least one band of the extracted spectrum. The quantified at least one peak of the extracted chromatogram and the quantified at least one band of the extracted spectrum are compared to a set of authentication criteria to determine authenticity of the botanical.

Abstract: The present invention provides the use of charged surface reversed phase chromatographic materials along with standard reversed-phase LC and mass spectrometry compatible conditions for the retention, separation, purification, and characterization of acidic, polar molecules, including, but not limited to, organic acids, ?-amino acids, phosphate sugars, nucleotides, other acidic, polar biologically relevant molecules. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier.

Abstract: An apparatus for use in a chromatography system includes a microfluidic substrate having a fluidic channel configured as an analytical chromatographic column and a fluidic port on one side of the microfluidic substrate. The fluidic port opens at a head end of the analytical chromatographic column. A dried blood spot (DBS) collection device holds one or more dried biological samples. The DBS collection device is directly coupled to the microfluidic substrate whereby one of the biological samples is placed into fluidic communication with the fluidic channel of the microfluidic substrate and an extraction of that biological sample flows toward the head end of the analytical chromatographic column. A diluent source fluidically coupled to the fluidic port supplies a solvent to the head end of the analytical column to dilute the extracted biological sample before the biological sample flows into the analytical chromatographic column.

Abstract: Provided herein are methodologies where a glycosylated protein or peptide is subjected to peptide bond cleavage to produce a glycan amino acid complex wherein the N-linked or O-linked glycan is attached. A derivatization reagent is then attached to the N terminus of the amino acid to provide a labeled glycan amino acid complex. The labeled glycan amino acid complex is then separated from the matrix via one or more methods including HILIC SPE, and injected directly onto an LC or LC/MS system for analysis, detection and characterization of the glycosylated protein or the peptide.

Abstract: An apparatus for use in a chromatography system includes a microfluidic substrate having a fluidic channel configured as an analytical chromatographic column and a fluidic port on one side of the microfluidic substrate. The fluidic port opens at a head end of the analytical chromatographic column. A dried blood spot (DBS) collection device holds one or more dried biological samples. The DBS collection device is directly coupled to the microfluidic substrate whereby one of the biological samples is placed into fluidic communication with the fluidic channel of the microfluidic substrate and an extraction of that biological sample flows toward the head end of the analytical chromatographic column. A diluent source fluidically coupled to the fluidic port supplies a solvent to the head end of the analytical column to dilute the extracted biological sample before the biological sample flows into the analytical chromatographic column.

Abstract: An electrospray emitter assembly (4) for an interface device (1) used to connect the output of a liquid chromatograph to the inlet of an electrospray ion source of a mass spectrometer (10). The device (1) incorporates an electrospray emitter assembly (4) that includes an emitter needle (40), a sheath (6) and a housing (5) with an attachment means in the form of a flange (55) for attachment to a mass spectrometer (10). The sheath (6) is arranged to shield the electrospray emitter needle (40) when the assembly is not attached to a mass spectrometer (10). The assembly (4) is operable to move the sheath (6) thereby to reveal, in use, the electrospray emitter needle (40) when the assembly (4) is attached to the mass spectrometer (10).