Abstract: The present invention describes a versatile, robust and environmentally controlled platform with a linear temperature gradient for massively parallel chemical or biochemical processing. This apparatus is capable of probing the phase transition behavior of macromolecules in solution, both thermodynamically and kinetically. This includes—but is not limited to—liquid/liquid phase transition behavior of antibody solutions and in situ gelation of thermo-responsive polymers. The device can be operated in a multiplex fashion using a controlled temperature gradient architecture and visualized by dark field microscopy or by other optical intensity measurements.

Abstract: A microfluidic chip includes multiple microfluidic channels each in fluid communication with a corresponding inlet. The inlets have a first spacing along a first linear path, wherein a central region of the microfluidic channels has a second spacing along a second linear path. The first spacing is greater than the second spacing. A microfluidic chip cassette is also disclosed.

Abstract: The present invention provides improved methods for characterizing kinetics and thermodynamics of solutions containing macromolecules over a range of concentrations, temperatures, pH, and added excipients in order to improve their long term stability.

Abstract: The present invention describes a versatile, robust and environmentally controlled platform with a linear temperature gradient for massively parallel chemical or biochemical processing. This apparatus is capable of probing the phase transition behavior of macromolecules in solution, both thermodynamically and kinetically. This includes- but is not limited to- liquid/liquid phase transition behavior of antibody solutions and in situ gelation of thermo-responsive polymers. The device can be operated in a multiplex fashion using a controlled temperature gradient architecture and visualized by dark field microscopy or by other optical intensity measurements.

Abstract: Methods and systems for detecting binding between first and second molecules using a pH-sensitive fluorophore. A change in fluorescence emission intensity of the fluorophore is indicative of binding.

Abstract: Method for sensing the binding of an analyte to a surface using a pH-sensitive fluorescent agent having a first fluorescence emission intensity at a first pH and a second fluorescence emission intensity at a second pH, and surfaces for sensing the binding of an analyte to a surface using the pH-sensitive fluorescent agent.

Abstract: The present disclosure relates to detecting receptor-ligand binding by measuring local pH modulation using a pH-sensitive fluorophore.

Abstract: Methods and systems for detecting binding between first and second molecules using a pH-sensitive fluorophore. A change in fluorescence emission intensity of the fluorophore is indicative of binding.

Abstract: The present disclosure relates to detecting receptor-ligand binding by measuring local pH modulation using a pH-sensitive fluorophore.

Abstract: The present invention is an apparatus for providing a linear temperature gradient to an architecture suitable for massively parallel chemical or biochemical processing. The architecture is disposed on a substrate. The apparatus uses two temperature elements disposed essentially parallel to each other and in thermal contact with the substrate. When the temperature elements are held at different temperatures, a linear temperature gradient is formed in the substrate.