Abstract: Methods, systems, and computer readable storage media relate to processing and analyzing a sample to determine presence or concentration of at least one sensor target in a sample. A method of quantifying a sample using a sensor may include obtaining sensor data, the sensor data including in-situ calibration data from a housekeeping protein; analyzing the sensor data; and outputting results of the analyzing. The method may be used with a system that includes a sensing device and a module. The system may also include an analysis device that can be in communication with the sensing device.

Abstract: Methods, systems, and computer readable storage media relate to processing and analyzing a sample to determine presence or concentration of at least one sensor target in a sample. A method of quantifying a sample using a sensor may include obtaining sensor data, the sensor data including in-situ calibration data from a housekeeping protein; analyzing the sensor data; and outputting results of the analyzing. The method may be used with a system that includes a sensing device and a module. The system may also include an analysis device that can be in communication with the sensing device.

Abstract: The present disclosure describes DNA damage endonucleases which exhibit broad specificity with respect to the types of structural aberrations in double stranded DNA. These enzymes recognize double stranded DNA with distortions in structure, wherein the distortions result from photoproducts, alkylation, intercalation, abasic sites, mismatched base pairs, insertion deletion loops, cisplatin adducts and other types of base damage (for example, uracil resulting from cytosine deamination). The UVDE (Uve1p) of Schizosaccharomyces pombe, certain truncated forms of that UVDE (lacking from about 100 to about 250 amino acids of N-terminal sequence) and certain endonucleases from Homo sapiens, Neurospora crassa, Bacillus subtilis, Bacillus anthracis, Methanococcus jannaschii, and Deinococcus radiodurans. The present disclosure further provides methods for cleaving double stranded DNA having structural distortions as set forth herein using the exemplified endonucleases or their stable, functional truncated derivatives.

Abstract: The present disclosure describes DNA damage endonucleases which exhibit broad specificity with respect to the types of structural aberrations in double stranded DNA. These enzymes recognize double stranded DNA with distortions in structure, wherein the distortions result from photoproducts, alkylation, intercalation, abasic sites, mismatched base pairs, cisplatin adducts and inappropriately incorporated bases (for example, 8-oxoguanine, inosine, xanthine, among others). The UVDE (Uve1p) of Schizosaccharomyces pombe, certain truncated forms of that UVDE (lacking from about 100 to about 250 amino acids of N-terminal sequence) and certain endonucleases from Homo sapiens, Neurospora crassa, Bacillus subtilis, and from Deinococcus radiodurans. The present disclosure further provides methods for cleaving double stranded DNA having structural distortions as set forth herein using the exemplified endonucleases or their stable, functional truncated derivatives.