Patents by Inventor Paul W. Tooley

Paul W. Tooley has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 6316195
    Abstract: Karnal bunt of wheat is caused by Tilletia indica Mitra. Recently, teliospores morphologically resembling T. indica were isolated from bunted ryegrass seeds and wheat seed washes. Previously developed PCR assays failed to differentiate T. indica from the newly discovered ryegrass pathogen, T. walkeri. The nucleotide sequence of a 2.3-kb region of mitochondrial DNA, previously amplified by PCR only from T. indica, was determined for three isolates of T. indica and for three isolates of T. walkeri. There was greater than 99% identity within either the T. indica group or the T. walkeri group of isolates, whereas there was approximately 3% divergence between isolates of these two Tilletia species. Five sets of PCR primers were made specific to T. indica, and three sets were designed specifically for T. walkeri based upon nucleotide differences within the mtDNA region.
    Type: Grant
    Filed: December 23, 1999
    Date of Patent: November 13, 2001
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventors: Reid D. Frederick, Paul W. Tooley, Morris R. Bonde, David A. Knorr, Gary L. Peterson, Norman W. Schaad
  • Patent number: 5811240
    Abstract: Mitochondrial DNA of five isolates of Tilletia indica was isolated and digested with several restriction enzymes. A 2.3 kb- EcoRI fragment was chosen, cloned, and shown to hybridize with total DNA restricted with EcoRI from T. indica and not from a morphologically similar smut fungus, T. barclayana. The clone was partially sequenced, primers were designed and tested under high-stringency conditions in PCR assays. The primer pair Ti1/Ti4 amplified a 2.3 kb fragment from total DNA of 17 T. indica isolates from India, Pakistan and Mexico. DNA from 25 isolates of other smut fungi (T. barclayana, T. foetida, T. caries, T. fusca and T. controversa) did not produce any bands as detected by ethidium bromide-stained agarose gels and Southern hybridizations. Sensitivity of the assay was determined and increased by using a single nested primer in a second round of amplification, so that 1 pg of total mycelial DNA could be detected.
    Type: Grant
    Filed: December 24, 1996
    Date of Patent: September 22, 1998
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventors: Marisa A. S. V. Ferreira, Paul W. Tooley, Efstathios Hatziloukas, Norman W. Schaad, Morris R. Bonde