Patents by Inventor Paul Yager

Paul Yager has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190285187
    Abstract: Temperature-actuated valves, devices including temperature-actuated valves, and related methods are described. In an embodiment, the temperature-actuated valve includes a heat-shrink film defining a perforation extending at least partially in a first direction. In an embodiment, the temperature-actuated valve is configured to open when a portion of the heat-shrink film including the perforation is heated above a threshold temperature to contract the heat-shrink film along a second direction perpendicular to the first direction to define an aperture, in an open configuration, providing a fluid a path through the heat-shrink film. In an embodiment, the temperature-actuated valve includes a leakage-mitigation feature configured to limit fluid flow through the perforation when the valve is in a closed configuration.
    Type: Application
    Filed: March 15, 2019
    Publication date: September 19, 2019
    Applicant: University of Washington
    Inventors: Paul Yager, Joshua Bishop, Michael Purfield
  • Publication number: 20190134637
    Abstract: The present technology is directed to capillarity-based devices for performing chemical processes and associated system and methods. In one embodiment, for example, a device can include a porous receiving element having an input region and a receiving region, a first fluid source and a second fluid source positioned within the input region of the receiving element; wherein the first fluid source is positioned between the second fluid source and the receiving region, and wherein, when both the first and second fluid sources are in fluid connection with the input region, the device is configured to sequentially deliver the first fluid and the second fluid to the receiving region without leakage.
    Type: Application
    Filed: August 9, 2018
    Publication date: May 9, 2019
    Inventors: Joshua Bishop, Joshua Buser, Samantha Byrnes, Shivani Dharmaraja, Elain S. Fu, Jared Houghtaling, Peter C. Kauffman, Sujatha Kumar, Lisa Lafleur, Tinny Liang, Barry Lutz, Bhushan Toley, Maxwell Wheeler, Paul Yager, Xiaohong Zhang
  • Publication number: 20180326418
    Abstract: The present technology is directed to capillarity-based devices for performing chemical processes and associated system and methods. In one embodiment, for example, a device can include a base configured to receive one or more fluids, a porous wick carried by the base portion, and a flow-metering element along the porous wick to modify a rate or volume of fluid flow along the porous wick. The porous wick can comprise a first pathway, a second pathway, and an intersection at which the first pathway and the second pathway converge. Input ends of the first and second pathways can be wettably distinct. Upon wetting of the input ends, fluid is configured to travel by capillary action along each pathway. The device may also include volume-metering features configured to automatically and independently control or modify a volume of fluid flow along one or more pathways of the porous wick.
    Type: Application
    Filed: May 14, 2018
    Publication date: November 15, 2018
    Applicant: University of Washington
    Inventors: Paul Yager, Barry R. Lutz, Elain S. Fu, Peter C. Kauffman
  • Publication number: 20180119202
    Abstract: The present technology is related to methods and compositions for detecting, and optionally quantifying, one or more analytes of a sample using nucleic acids. In some embodiments, the methods include generating a complex of a plurality of peptides, an analyte, a first nucleic acid, and a second nucleic acid, each nucleic acid conjugated to a binder peptide. In addition, an immobilizer peptide can be immobilized to a substrate. If the binder peptides are bound to the analyte, the method further includes hybridizing a segment of the first nucleic acid to a segment of the second nucleic acid and amplifying the hybridized nucleic acids to generate a plurality of amplicons. Moreover, the generated amplicons indicate that one or more analytes has been detected. A number of generated amplicons can be analyzed to quantify one or more of the bound analytes.
    Type: Application
    Filed: November 1, 2017
    Publication date: May 3, 2018
    Inventors: Paul Yager, Joshua Bishop, Erin Heiniger
  • Patent number: 9778261
    Abstract: The present invention provides porous membrane-binding polypeptides, fusion proteins thereof, and methods for use of the polypeptides and fusion proteins in binding assays.
    Type: Grant
    Filed: September 22, 2016
    Date of Patent: October 3, 2017
    Assignee: University of Washington
    Inventors: Paul Yager, Caitlin Anderson, David Baker, Yu-Ru Lin, Carly Holstein
  • Publication number: 20170131211
    Abstract: The present technology relates generally to systems for disrupting biological samples and associated devices and methods. In some embodiments, the system includes a vessel configured to receive a biological sample and a cap assembly that includes a porous membrane having a receiving region and a detection region. When the cap assembly is detachably coupled to an open end portion of the vessel, the system can be moved between a first orientation and a second orientation. When the system is in the first orientation, the biological sample is not in fluid communication with the receiving region. When the vessel contains is in the second orientation, the biological sample is in fluid communication with the receiving region and wicks through the porous membrane to the detection region.
    Type: Application
    Filed: November 10, 2016
    Publication date: May 11, 2017
    Inventors: Paul Yager, Joshua Bishop, Joshua Buser, Louise Lyth Hansen, Erin K. Heiniger, Enos Kline, Sujatha Kumar
  • Publication number: 20170082624
    Abstract: The present invention provides porous membrane-binding polypeptides, fusion proteins thereof, and methods for use of the polypeptides and fusion proteins in binding assays.
    Type: Application
    Filed: September 22, 2016
    Publication date: March 23, 2017
    Inventors: Paul YAGER, Caitlin ANDERSON, David BAKER, Yu-Ru LIN, Carly HOLSTEIN
  • Patent number: 9528987
    Abstract: The present technology describes various embodiments of devices for processing, analyzing, detecting, measuring, and separating fluids. The devices can be used to perform these processes on a microfluidic scale, and with control over fluid and reagent transport. In one embodiment, for example, a device for performing chemical processes can include a porous wick comprising a pathway defined by an input end, an output end, and a length between the input end and the output end. The pathway is configured to wick fluid from the input end to the output end by capillary action. The device can further include a reagent placed on the pathway. The reagent can be placed in a pattern configured to control a spatial or temporal distribution of the reagent along the pathway upon wetting of the pathway.
    Type: Grant
    Filed: June 25, 2012
    Date of Patent: December 27, 2016
    Assignee: University of Washington
    Inventors: Paul Yager, Barry R. Lutz, Elain S. Fu, Gina Fridley, Huy Quang Le, Peter C. Kauffman
  • Publication number: 20160310942
    Abstract: The present technology is directed to capillarity-based devices for performing chemical processes and associated system and methods. In one embodiment, for example, a device can include a first porous element having a first pore size and configured to receive a fluid at its proximal portion, and a second porous element having a second pore size greater than the first pore size and configured to receive a fluid at its proximal portion. The first porous element can be positioned across the second porous element such that an overlapping region exists between the porous elements where the porous elements are in fluid communication. Before delivery of the fluid to the second porous element, the fluid pressure at the overlapping region is greater than the capillary pressure of the second porous element such that a fluid delivered to the first porous element wicks through its overlapping portion without wetting the second porous element.
    Type: Application
    Filed: April 21, 2016
    Publication date: October 27, 2016
    Inventors: Paul Yager, Joshua Buser, Samantha Byrnes, Erin K. Heiniger, Peter C. Kauffman, Paula Ladd
  • Publication number: 20150361487
    Abstract: The present technology is directed to capillarity-based devices for performing chemical processes and associated system and methods. In one embodiment, for example, a device can include a porous receiving element having an input region and a receiving region, a first fluid source and a second fluid source positioned within the input region of the receiving element; wherein the first fluid source is positioned between the second fluid source and the receiving region, and wherein, when both the first and second fluid sources are in fluid connection with the input region, the device is configured to sequentially deliver the first fluid and the second fluid to the receiving region without leakage.
    Type: Application
    Filed: January 22, 2014
    Publication date: December 17, 2015
    Inventors: Joshua Bishop, Joshua Buser, Samantha Byrnes, Shivani Dharmaraja, Elain S. Fu, Jared Houghtaling, Peter C. Kauffman, Sujatha Kumar, Lisa Lafleur, Tinny Liang, Barry Lutz, Bhushan Toley, Maxwell Wheeler, Paul Yager, Xiaohong Zhang
  • Patent number: 9138743
    Abstract: Disclosed are methods and devices for rapid parallel molecular affinity assays performed in a microfluidic environment. The invention exploits hydrodynamic addressing to provide simultaneous performance of multiple assays in parallel using a minimal sample volume flowing through a single channel.
    Type: Grant
    Filed: January 5, 2012
    Date of Patent: September 22, 2015
    Assignee: UNIVERSITY OF WASHINGTON
    Inventors: Paul Yager, Turgut Fettah Kosar, Michael Wai-Haung Look, Afshin Mashadi-Hossein, Katherine McKenzie, Kjell E. Nelson, Paolo Spicar-Mihalic, Dean Stevens, Rahber Thariani
  • Publication number: 20150203806
    Abstract: The present technology relates generally to systems for disrupting biological samples and associated devices and methods. In some embodiments, system includes a vessel configured to receive the biological sample, a permanent magnet configured to be positioned within the vessel, an electromagnet configured to be positioned proximate the vessel, and a current source operably coupled to the electromagnet and configured to transmit an alternating current. In some embodiments, when the biological sample is placed within the vessel and the alternating current is transmitted to the electromagnet, the electromagnet produces an alternating magnetic field that causes the permanent magnet to rotate within the vessel, thereby lysing at least one of the cells of the biological sample.
    Type: Application
    Filed: January 21, 2015
    Publication date: July 23, 2015
    Inventors: Paul Yager, Peter C. Kauffman, Joshua Buser, Samantha Byrnes, Alec K. Wollen, Erin Heiniger
  • Publication number: 20140227707
    Abstract: The present technology describes various embodiments of devices for processing, analyzing, detecting, measuring, and separating fluids. The devices can be used to perform these processes on a microfluidic scale, and with control over fluid and reagent transport. In one embodiment, for example, a device for performing chemical processes can include a porous wick comprising a pathway defined by an input end, an output end, and a length between the input end and the output end. The pathway is configured to wick fluid from the input end to the output end by capillary action. The device can further include a reagent placed on the pathway. The reagent can be placed in a pattern configured to control a spatial or temporal distribution of the reagent along the pathway upon wetting of the pathway.
    Type: Application
    Filed: June 25, 2012
    Publication date: August 14, 2014
    Applicant: University of Washington Through itsCenter for Com
    Inventors: Paul Yager, Barry R. Lutz, Elain S. Fu, Gina Fridley, Huy Quang Le, Peter C. Kauffman
  • Publication number: 20120288961
    Abstract: The present technology is directed to capillarity-based devices for performing chemical processes and associated system and methods. In one embodiment, for example, a device can include a base configured to receive one or more fluids, a porous wick carried by the base portion, and a flow-metering element along the porous wick to modify a rate or volume of fluid flow along the porous wick. The porous wick can comprise a first pathway, a second pathway, and an intersection at which the first pathway and the second pathway converge. Input ends of the first and second pathways can be wettably distinct. Upon wetting of the input ends, fluid is configured to travel by capillary action along each pathway. The device may also include volume-metering features configured to automatically and independently control or modify a volume of fluid flow along one or more pathways of the porous wick.
    Type: Application
    Filed: December 21, 2010
    Publication date: November 15, 2012
    Applicant: University of Washington
    Inventors: Paul Yager, Barry R. Lutz, Elain S. Fu, Peter C. Kauffman
  • Publication number: 20120171698
    Abstract: Disclosed are methods and devices for rapid parallel molecular affinity assays performed in a microfluidic environment. The invention exploits hydrodynamic addressing to provide simultaneous performance of multiple assays in parallel using a minimal sample volume flowing through a single channel.
    Type: Application
    Filed: January 5, 2012
    Publication date: July 5, 2012
    Applicant: University of Washington
    Inventors: Paul Yager, Turgut Fettah Kosar, Michael Wai-Haung Look, Afshin Mashadi-Hossein, Katherine McKenzie, Kjell E. Nelson, Paolo Spicar-Mihalic, Dean Stevens, Rahber Thariani
  • Publication number: 20120138469
    Abstract: Devices and methods are provided for separation of particles of a first selected electrophoretic mobility or isoelectric point from a fluid comprising particles of at least one other selected electrophoretic mobility or isoelectric point. The devices comprise a microchannel; electrodes to either side of the microchannel for applying a selected voltage to produce an electrical field across the microchannel orthogonal to the length of the microchannel; and outlets in said microchannel placed to receive outlet portions of the fluid containing enhanced concentrations of each type of particle. The devices may be used for particle detection, quantification, separation, mixing, dilution and concentration; to release, separate and detect interior particles of cells or organisms, and to separate particles such as proteins and microorganisms from biological fluids such as blood; or to separate and detect airborne contaminants such as bacterial warfare agents from air.
    Type: Application
    Filed: November 13, 2007
    Publication date: June 7, 2012
    Applicant: University of Washington
    Inventors: Paul Yager, Mark Holl, Darrel J. Bell, James Brody, Catherine R. Cabrera, Andrew E. Kamholz, Katerina Macounova, Dong Qin
  • Patent number: 8101403
    Abstract: Disclosed are methods and devices for rapid parallel molecular affinity assays performed in a microfluidic environment. The invention exploits hydrodynamic addressing to provide simultaneous performance of multiple assays in parallel using a minimal sample volume flowing through a single channel.
    Type: Grant
    Filed: October 4, 2007
    Date of Patent: January 24, 2012
    Assignee: University of Washington
    Inventors: Paul Yager, Turgut Fettah Kosar, Michael Wai-Haung Look, Afshin Mashadi-Hossein, Katherine McKenzie, Kjell E. Nelson, Paolo Spicar-Mihalic, Dean Y. Stevens, Rahber Thariani
  • Publication number: 20110151479
    Abstract: Disclosed is a flow-through membrane assay that takes advantage of a high surface area and rapid transport while allowing individual control over flow rates and times for each step of a multi-step assay. A microfluidic card features channels in communication with a porous membrane, channels on either side of membrane to allow transverse flow across the membrane, capturing a labeled target from the sample by flowing the sample across the membrane, or capturing a target from the sample followed by flowing a reagent containing a label that binds to the target. Fluid can be pushed or pulled through the assay membrane by external control. Air near the membrane is managed by diverting air between fluids to a channel upstream of the assay membrane, venting air between fluids through a hydrophobic membrane upstream of the assay membrane, and/or by venting trapped air through a hydrophobic membrane downstream of the assay membrane.
    Type: Application
    Filed: August 25, 2009
    Publication date: June 23, 2011
    Applicant: University of Washington
    Inventors: Dean Y. Stevens, Lisa K. Lafleur, Berry R. Lutz, Paolo Spicar-Mihalic, Paul Yager
  • Patent number: 7736891
    Abstract: Disclosed is a microfluidic assay system and methods that apply flow injection analysis to permit dispersion monitoring. A solution containing a reagent that binds an analyte and a tracer is delivered via pressure-driven flow into the receiving end of the injection channel of the system of the invention. A sample fluid suspected of containing the analyte is delivered into the upstream end of the input channel under conditions permitting flow of the sample fluid toward the downstream end of the assay channel and permitting dispersion of the reagent into the sample fluid. The amount of tracer present in the fluid as it passes over the reference region and the capture region and the amount of binding between the analyte and the capture region are detected. The amount of binding detected between the analyte and the capture region is correlated to the amount of tracer detected in the reference region.
    Type: Grant
    Filed: September 11, 2008
    Date of Patent: June 15, 2010
    Assignee: University of Washington
    Inventors: Kjell E. Nelson, Paul Yager
  • Publication number: 20100081216
    Abstract: Disclosed are methods and devices for rapid parallel molecular affinity assays performed in a microfluidic environment. The invention exploits hydrodynamic addressing to provide simultaneous performance of multiple assays in parallel using a minimal sample volume flowing through a single channel.
    Type: Application
    Filed: October 4, 2007
    Publication date: April 1, 2010
    Applicant: Univeristy of Washington
    Inventors: Paul Yager, Turgut Fettah Kosar, Michael Wai-Haung Look, Afshin Mashadi-Hossein, Katherine McKenzie, Kjell E. Nelson, Paolo Spicar-Mihalic, Dean Y. Stevens, Rahber Thariani