Abstract: Systems and methods for detecting microbial toxins are disclosed. The system includes an imaging device operable to detect a luminescent signal and an analysis plate having a well to hold a sample containing the microbial toxin. The luminescence is created by a cell line expressing a product capable of reacting directly or indirectly with the microbial toxin to produce the luminescent signal. The signal is processed via an image processing system operable to receive the luminescent signal detected by the imaging device and convert the luminescent signal to a quantitative measurement correlated to an amount of the microbial toxin present in the sample.

Abstract: Systems and methods for detecting microbial toxin in a food matrix are disclosed. The system includes an imaging device operable to detect a luminescent signal and an analysis plate having a well to hold a sample containing the microbial toxin. The luminescence is created by a cell line having a reporter gene expressing a product capable of reacting directly or indirectly with the microbial toxin to produce the luminescent signal. The system includes an optional accessory cell line which when exposed to the microbial toxin displays the microbial toxin as an antigen on its surface to activate expression of the reporter gene expressing the product capable of reacting directly or indirectly with the microbial toxin to produce the luminescent signal. The signal is processes via an image processing system operable to receive the luminescent signal detected by the imaging device and convert the luminescent signal to a quantitative measurement correlated to an amount of the microbial toxin present in the sample.