Patents by Inventor Paula M. Keith

Paula M. Keith has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 5104796
    Abstract: A high titer fermentation process using transformed Escherichia coli cells carrying a plasmid containing DNA which codes for Somatomedin C is disclosed. Titers of about 900-1000 mg/L have been obtained using the method of the present invention; about two times the titer of prior art processes.
    Type: Grant
    Filed: April 6, 1987
    Date of Patent: April 14, 1992
    Assignee: International Minerals & Chemical Corp.
    Inventors: Paula M. Keith, Wendy Cain
  • Patent number: 4762784
    Abstract: A high density fermentation process for high yield production of bovine growth hormone by transformant E. coli is described. The process employs transformant strains of E. coli containing an expression vector coding for bovine growth hormone under the control of a bacteriophage lambda promoter-operator and a plasmid containing the .lambda.cI857 gene which codes for the temperature-sensitive repressor protein. In the initial growth period, the level of dissolved oxygen in the fermentation medium is maintained at about 20% to 60% saturation and the temperature of the medium is kept at 26.degree.-30.degree. C. Production of bovine growth hormone is then induced by raising the temperature of the medium to at least about 42.degree. C. The temperature is then reduced to about 38.degree. C. to 41.degree. C. to optimize cell growth for the remainder of the induction period, during which the level of dissolved oxygen in the medium is maintained at about 10% to 40% saturation.
    Type: Grant
    Filed: July 15, 1985
    Date of Patent: August 9, 1988
    Assignee: International Minerals & Chemical Corp.
    Inventors: Paula M. Keith, Wendy J. Cain