Patents by Inventor Paulien Neefe

Paulien Neefe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Variant CBH1 cellulases
    Patent number: 9896674
    Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
    Type: Grant
    Filed: January 21, 2016
    Date of Patent: February 20, 2018
    Assignee: DANISCO US INC
    Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
  • CBH1 homologs and varian CBH1 cellulase
    Patent number: 8765440
    Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
    Type: Grant
    Filed: March 25, 2011
    Date of Patent: July 1, 2014
    Assignee: Danisco US Inc.
    Inventors: Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe
  • Variant CBH2 cellulases
    Patent number: 8715997
    Abstract: Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
    Type: Grant
    Filed: July 7, 2011
    Date of Patent: May 6, 2014
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Frits Goedegebuur, Lydia Dankmeyer, Colin Mitchinson, Paulien Neefe, Bradley Kelemen, Robert Caldwell, Pauline Teunissen
  • OVER EXPRESSION OF FOLDASES AND CHAPERONES IMPROVES PROTEIN PRODUCTION
    Publication number: 20140087443
    Abstract: The present teachings provide methods for increasing protein secretion, e.g., chymosin in filamentous fungi by co-expressing certain chaperone(s) and/or foldase(s). The present teachings also provide filamentous fungi containing certain chaperone(s) and/or foldase(s) and a protein of interest for increased secretion.
    Type: Application
    Filed: August 27, 2013
    Publication date: March 27, 2014
    Applicant: Danisco US Inc.
    Inventors: Frits Goedegebuur, Paulien Neef-Kruithof, Jeffrey P. Pucci, Michael Ward
  • Variant CBH1 cellulase
    Patent number: 8679817
    Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
    Type: Grant
    Filed: June 14, 2012
    Date of Patent: March 25, 2014
    Assignee: Danisco US Inc.
    Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe-Kruithof, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
  • Variant CBH1 cellulases
    Patent number: 8637294
    Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
    Type: Grant
    Filed: March 22, 2007
    Date of Patent: January 28, 2014
    Assignee: Danisco US Inc.
    Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
  • Over expression of foldases and chaperones improves protein production
    Patent number: 8546126
    Abstract: The present teachings provide methods for increasing protein secretion, e.g., chymosin in filamentous fungi by co-expressing certain chaperone(s) and/or foldase(s). The present teachings also provide filamentous fungi containing certain chaperone(s) and/or foldase(s) and a protein of interest for increased secretion.
    Type: Grant
    Filed: March 21, 2008
    Date of Patent: October 1, 2013
    Assignee: Danisco US Inc.
    Inventors: Frits Goedegebuur, Paulien Neefe-Kruithof, Jeffrey P. Pucci, Michael Ward
  • glucoamylase and homologs thereof
    Patent number: 8349601
    Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.
    Type: Grant
    Filed: March 16, 2010
    Date of Patent: January 8, 2013
    Assignee: Danisco US Inc.
    Inventors: Nigel Dunn-Coleman, Paulien Neefe-Kruithof, Craig E. Pilgrim, Piet van Solingen, Donald E. Ward
  • NOVEL VARIANT HYPROCREA JECORINA CBH1 CELLULASES
    Publication number: 20120270298
    Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
    Type: Application
    Filed: June 14, 2012
    Publication date: October 25, 2012
    Applicants: GENENCOR INTERNATIONAL, INC., DANISCO US INC.
    Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
  • Variant CBH1 cellulases
    Patent number: 8232080
    Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
    Type: Grant
    Filed: May 13, 2011
    Date of Patent: July 31, 2012
    Assignee: Danisco US Inc.
    Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
  • Novel Variant Hypocrea Jecorina CBH2 Cellulases
    Publication number: 20120040435
    Abstract: Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
    Type: Application
    Filed: July 7, 2011
    Publication date: February 16, 2012
    Applicant: Danisco US Inc.
    Inventors: Wolfgang Aehle, Frits Goedegebuur, Lydia Dankmeyer, Colin Mitchinson, Paulien Neefe, Bradley Kelemen, Robert Caldwell, Pauline Teunissen
  • Novel Variant Hyprocrea Jecorina CBH1 Cellulases
    Publication number: 20110229956
    Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
    Type: Application
    Filed: May 13, 2011
    Publication date: September 22, 2011
    Applicants: Danisco US Inc., Genencor International, Inc.
    Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
  • Variant CBH2 cellulases
    Patent number: 8008056
    Abstract: Described herein are variants of H. jecorina CBH2, a Cel6A enzyme. The present invention provides novel cellobiohydrolases that have altered thermostability.
    Type: Grant
    Filed: December 22, 2005
    Date of Patent: August 30, 2011
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Frits Goedegebuur, Lydia Dankmeyer, Colin Mitchinson, Paulien Neefe, Brad Kelemen, Robert Caldwell, Pauline Teunissen
  • Novel CBH1 Homologs And Varian CBH1 Cellulase
    Publication number: 20110177561
    Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
    Type: Application
    Filed: March 25, 2011
    Publication date: July 21, 2011
    Applicant: Danisco US Inc.
    Inventors: Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe
  • Variant CBH1 cellulases
    Patent number: 7972832
    Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
    Type: Grant
    Filed: August 15, 2003
    Date of Patent: July 5, 2011
    Assignee: Danisco US Inc.
    Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
  • CBH1 homologs and variant CBH1 cellulases
    Patent number: 7951570
    Abstract: Disclosed are a number of homologs and variants of Hypocrea jecorina Cel7A (formerly Trichoderma reesei cellobiohydrolase I or CBH1), nucleic acids encoding the same and methods for producing the same. The homologs and variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted and/or deleted.
    Type: Grant
    Filed: October 13, 2008
    Date of Patent: May 31, 2011
    Assignee: Danisco US Inc.
    Inventors: Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe
  • glucoamylase and homologs thereof
    Patent number: 7919299
    Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.
    Type: Grant
    Filed: January 14, 2009
    Date of Patent: April 5, 2011
    Assignee: Danisco US Inc.
    Inventors: Nigel Dunn-Coleman, Paulien Neefe-Kruithof, Craig E. Pilgrim, Piet Van Solingen, Donald E. Ward
  • Trichoderma Reesei Glucoamylase and Homologs Thereof
    Publication number: 20110033900
    Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.
    Type: Application
    Filed: March 16, 2010
    Publication date: February 10, 2011
    Inventors: Nigel Dunn-Coleman, Paulien Neefe-Kruithof, Craig E. Pilgrim, Piet Van Solingen, Donald E. Ward
  • OVER EXPRESSION OF FOLDASES AND CHAPERONES IMPROVES PROTEIN PRODUCTION
    Publication number: 20100144013
    Abstract: The present teachings provide methods for increasing protein secretion, e.g., chymosin in filamentous fungi by co-expressing certain chaperone(s) and/or foldase(s). The present teachings also provide filamentous fungi containing certain chaperone(s) and/or foldase(s) and a protein of interest for increased secretion.
    Type: Application
    Filed: March 21, 2008
    Publication date: June 10, 2010
    Inventors: Frits Goedegebuur, Paulien Neefe-Kruithof, Jeffrey P. Pucci, Michael Ward
  • glucoamylase and homologs thereof
    Patent number: 7723079
    Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.
    Type: Grant
    Filed: July 21, 2008
    Date of Patent: May 25, 2010
    Assignee: Genencor International, Inc.
    Inventors: Nigel Dunn-Coleman, Paulien Neefe-Kruithof, Craig E. Pilgrim, Piet Van Solingen, Donald E. Ward, III