Abstract: Providedherein are compositions for cryopreservation of mammalian cells, tissues, and organs. The compositions include a necroptosis inhibitor compound and a Bax channel inhibitor compound. Provided herein are also methods of use of the compositions, for treating, preventing, inhibiting, or reducing the incidence of cellular plasticity, or necroptosis or necrosis, of a plurality of cells, wherein the cells are brought into contact with the composition.

Abstract: The present invention relates to a method for predicting the tendency of a protein to form amphiphilic .alpha. or .beta. structure, wherein a series of values for U are calculated for spans of x residues, where the equationU.sub..alpha.x =H.sub.x +.mu..sub..alpha.x -<pt>is used to predict regions of amphiphilic .alpha. structure and the equationU.sub..beta.x =H.sub.x +.mu..sub..beta.x <pt>is used to predict regions of amphiphilic .beta. structure, and where H.sub.x is the average hydrophobicity for a span of x residues, .mu..sub.x is the hydrophobic moment, and <pt> is the position dependent turn propensity. When the values for U.sub..alpha.x and U.sub..beta.x are represented graphically, peaks are predicted to represent regions of .alpha. and .beta. structure, respectively.