Patents by Inventor Pavel Klener

Pavel Klener has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of cultivation of human mesenchymal stem cells, particularly for the treatment of non-healing fractures, and bioreactor for carrying out this cultivation method
    Publication number: 20090305406
    Abstract: The invention relates to a novel method of cultivation of mesenchymal stem cells, wherein after aseptic separation of mononuclear cells from the marrow blood, said cells are seeded in low density into sterile plastic cultivation vessels and cultivated for approximately one to three weeks in CellGro™ Hematopoietic Stem Cell Medium, certified for the clinical use, with an addition of 10% human serum and supplements, wherein the supplements are added at least once in the course of the cultivation, without removal of hematopoietic cells and without medium exchange during the cultivation procedure, without any interference with the closed cultivation system, under the standard conditions for the cultivation of tissue cultures. For the cultivation of the mesenchymal stem cells in the closed cultivation system for the clinical use in the field of orthopaedic surgery, a simple bioreactor is proposed.
    Type: Application
    Filed: January 23, 2007
    Publication date: December 10, 2009
    Inventors: Robert Pytlik, Petr Hofman, Tomas Trc, David Stehlik, Tomas Soukup, Petr Kobylka, Pavel Klener, Frantisek Rypacek, Katarina Mulinkova