Abstract: The computer-controlled optical scanning high magnification microscope imaging system with a large field of view disclosed herein overcomes a previous inability to achieve simultaneous high magnification and large field of view in microscopes. The subject imaging system includes galvanometer scanners, a CCD camera and high brightness LED sources at different wavelengths for rapid acquisition of a large number of high-resolution segmented tile images with a magnification of 800× each. The numerous segmented tiles combine to form a larger viewing field of the target, resulting in a compound image with an effective enlarged viewing area of 1.6×1.2 mm2. The speed and sensitivity of the system make it suitable for high resolution image monitoring of a small segmented area of dimensions 320×240 ?m2 with a 4 ?m resolution. The microscope can zoom in on each segment of the target without lost of resolution to attain a great degree of spatial detail.