Abstract: The invention provides a chemical compound library containing a collection of chromatographic fractions purified from biological sources suitable for high-throughput screening for biological activity, wherein: (a) the chromatographic fractions contain primarily from about 1 microgram to 100 micrograms of primarily one to five chemical compounds; (b) the compounds have log P of greater than-1 and less than 5 and molecular weights less than 3,000 Daltons; (c) the library can include chemical compounds produced from at least fifty different biological sources; and (d) the library can contain at least about 200 different chemical compounds from each different biological source.