Abstract: Continuous direct enzymatic protein solubilization process for industrial wastes. The present invention involves a sequential enzymatic process which enables solubilizing proteins that are present in determined industrial wastes, permitting the use of by-products. The wastes used can be of plant origin (rice bran, algarroba seed, etc.) or animal origin (Procambarus clarkii or American crab). The main process includes two steps: a first step of successive washings with acidified water and a second step of continuous enzymatic hydrolysis. Variants to the main process are shown without pretreatment of the wastes with acidified water. These solubilized protein wastes (peptones) have an application, among others, in the formulation of culture media, in the biosynthesis of pharmaceutical and food products, in clinical or dietetic nutrition, as additives in cosmetics and as components of organic fertilizers.

Abstract: Continuous direct enzymatic protein solubilization process for industrial waste. The invention consists of obtaining peptones (mixture of proteins, peptides and amino acids) starting from industrial wastes of plant origin. The process is carried out in two steps. On the first step the wastes are subjected to successive washes with acidified water to wash out alkaloids (polyphenols), sugars, insoluble fibers, etc., a concentrate rich in proteins being obtained. In a continuous process said protein concentrate is next subjected to a second enzymatc hydrolysis step (25-40%) with endoproteases, the peptones being obtained which involve a degree of solubilization in the order of 60-80% of the insoluble commencement proteins.

Abstract: Continuous direct enzymatic protein solubilization process for industrial wastes. The present invention consists of a sequential enzymatic process which enables solubilizing proteins that are present in determined industrial wastes, permitting the use of by-products. The wastes used can be of plant origin (rice bran, algarroba seed, etc.) or animal origin (Procambarus clarkii or American crab). The main process comprises two steps: a first step of successive washings with acidified water and a second step of continuous enzymatic hydrolysis. Variants to the main process are disclosed without pretreatment of the wastes with acidified water. These solubilized protein wastes (peptones) have an application, among others, in the formulation of culture media, in the biosynthesis of pharmaceutical and food products, in clinical or dietetic nutrition, as additives in cosmetics and as components of organic fertilizers.

Abstract: Continuous direct enzymatic protein solubilisation process for industrial waste. The invention consists of obtaining peptones (mixture of proteins, peptides and amino acids) starting from industrial wastes of plant origin. The process is carried out in two steps. On the first step the wastes are subjected to successive washes with acidified water to wash out alcaloids (polyphenols), sugars, insoluble fibres, etc., a concentrate rich in proteins being obtained. In a continuous process said protein concentrate is next subjected to a second enzymatc hydrolysis step (25-40%) with endoproteases, the peptones being obtained which involve a degree of solubilisation in the order of 60-80% of the insoluble commencement proteins.