Abstract: The present invention concerns a method and a system for imaging at least a part of a specimen by means of two microscopy imaging methods, where a surface (11) of the specimen (10) is imaged by means of a first microscopy imaging method, where a replica (25) of the surface (11) to be imaged by means of the first microscopy imaging method is produced and this replica (25) is simultaneously imaged by means of a second microscopy imaging method, where the images produced by means of the first and the second microscopy imaging methods are overlaid at the correct scale.

Abstract: A method is proposed for generating a series of ultra-thin sections of a microscopic sample (10), wherein the sections (11) are detached from the sample (10) using an ultramicrotome (100) and wherein the sections (11), which are detached from the sample (10) are caused to float on a liquid surface and are thereafter transferred onto a solid carrier element (20). For at least for some of the sections (11) detached from the sample (10) a position and an orientation on the solid carrier element (20) are determined by monitoring the placement of these sections (11) onto the solid carrier element (20) using a monitoring system (400) comprising a camera (410), obtaining monitoring data. A method (2000) for the three-dimensional reconstruction of a microscopic sample (10), a microtome system and a computer program are also part of the present invention.

Abstract: The present invention concerns a method and a system for imaging at least a part of a specimen by means of two microscopy imaging methods, where a surface (11) of the specimen (10) is imaged by means of a first microscopy imaging method, where a replica (25) of the surface (11) to be imaged by means of the first microscopy imaging method is produced and this replica (25) is simultaneously imaged by means of a second microscopy imaging method, where the images produced by means of the first and the second microscopy imaging methods are overlaid at the correct scale.

Abstract: A method is proposed for generating a series of sections of a microscopic sample (10), in which the sections are detached from the sample (10) by means of a blade (21), collected, and placed onto a transfer device. A sequence in which the sections are detached from the sample is detected; detachment of the sections from the sample (10) and/or collection of the sections and/or placement of the sections onto the transfer device is monitored by means of one or several observation cameras, accompanied by the acquisition of moving-image data; the sections are tracked in the moving-image data; and positions of the sections on the transfer device are correlated, on the basis of the tracking in the moving-image data, with the sequence in which they were detached from the sample.

Abstract: A method is proposed for generating a series of sections of a microscopic sample (10), in which the sections are detached from the sample (10) by means of a blade (21), collected, and placed onto a transfer device. A sequence in which the sections are detached from the sample is detected; detachment of the sections from the sample (10) and/or collection of the sections and/or placement of the sections onto the transfer device is monitored by means of one or several observation cameras, accompanied by the acquisition of moving-image data; the sections are tracked in the moving-image data; and positions of the sections on the transfer device are correlated, on the basis of the tracking in the moving-image data, with the sequence in which they were detached from the sample.