Abstract: The invention discloses a method for inhibiting the growth of cancer cells by use of an anti-cancer composition containing a conditioned cell culture medium from mesenchymal stem cells and cytokines. It comprises the steps of applying a composition with a conditioned cell culture medium from stem cells and at least one cytokine to cancer cells for growth inhibition of the cancer cells. The cell culture medium can be conditioned with Wharton's Jelly mesenchymal stem cells (WJMSCs) as an WJMSCs-conditoned cell culture medium, and the at least one cytokine is selected from a group consisting of bone morphogenetic protein-4, Dickkopf-related protein, Interferon-? and tumor necrosis factor-related apoptosis-inducing ligand.

Abstract: The present invention disclosed a method for preparing of collagen having regeneration and repair effects from Wharton's Jelly mesenchymal stein cells, comprising steps of culturing the Wharton's Jelly mesenchymal stein cells in a first medium for 16 to 24 hours; replacing the first medium with a second medium for culturing the Wharton's Jelly mesenchymal stein cells for 36 to 48 hours; collecting the Wharton's Jelly mesenchymal stein cells and adding a cell lysis solution to lyse the Wharton's Jelly mesenchymal stein cells for 0.5 to 2 hours, adding an inorganic salt solution to the cell lysis solution to obtain a mixing solution for further incubation at 4° C. for 24 to 48 hours; centrifuging the mixing solution and collecting a sediment, dissolving the sediment by a preservation solution to obtain a collage.

Abstract: The present invention relates to a method for avoidance of blue light damage by using a stem cell composition. The stem cell composition comprises 10% (v/v) to 50% (v/v) of the conditioned medium of Wharton's Jelly mesenchymal stem cells. The conditioned medium of Wharton's Jelly mesenchymal stem cells is prepared by culturing Wharton's Jelly mesenchymal stem cells in a medium containing human basic fibroblast growth factors for 2 to 5 days, and collecting the medium for centrifugation and filtration.

Abstract: The present invention disclosed a method for preparing of collagen having regeneration and repair effects from Wharton's Jelly mesenchymal stein cells, comprising steps of culturing the Wharton's Jelly mesenchymal stein cells in a first medium for 16 to 24 hours; replacing the first medium with a second medium for culturing the Wharton's Jelly mesenchymal stein cells for 36 to 48 hours; collecting the Wharton's Jelly mesenchymal stein cells and adding a cell lysis solution to lyse the Wharton's Jelly mesenchymal stein cells for 0.5 to 2 hours, adding an inorganic salt solution to the cell lysis solution to obtain a mixing solution for further incubation at 4° C. for 24 to 48 hours; centrifuging the mixing solution and collecting a sediment, dissolving the sediment by a preservation solution to obtain a collage.

Abstract: A use of a stem cell conditioned medium to inhibit oxidation for anti-aging skin. First, mesenchymal stem cells are cultured in a cell culture dish containing a complete growth medium. After mesenchymal stem cells are sub-cultured in the complete growth media for three times and transferred to a basal medium, a conditioned medium can be acquired from the basal medium.

Abstract: A use of a stem cell conditioned medium to induce ZO-1 proteins expression for skin regeneration, repair and finning is revealed herein. First, mesenchymal stem cells are cultured in a cell culture dish containing complete growth media, wherein the complete growth media include ?-MEM, fetal bovine serum, and human-basic fibroblast growth factors. After mesenchymal stem cells are sub-cultured in the complete growth media for three times, a conditioned medium which can effectively increase the activation of tight junction protein Zonula occludens-1 (ZO-1) can be acquired from the basal medium.

Abstract: A use of a stem cell conditioned medium to inhibit melanin formation for skin whitening is revealed. First, mesenchymal stem cells are cultured in a cell culture dish containing complete growth media. After mesenchymal stem cells are sub-cultured in a complete growth media for three times, a conditioned medium can be acquired from the basal media.

Abstract: A use of a stem cell conditioned medium to inhibit oxidation for anti-aging skin. First, mesenchymal stem cells are cultured in a cell culture dish containing a complete growth medium. After mesenchymal stem cells are sub-cultured in the complete growth media for three times and transferred to a basal medium, a conditioned medium can be acquired from the basal medium.

Abstract: A use of a stem cell conditioned medium to inhibit melanin formation for skin whitening is revealed. First, mesenchymal stem cells are cultured in a cell culture dish containing complete growth media. After mesenchymal stem cells are sub-cultured in a complete growth media for three times, a conditioned medium can be acquired from the basal media.

Abstract: A use of a stem cell conditioned medium to induce ZO-1 proteins expression for skin regeneration, repair and finning is revealed herein. First, mesenchymal stem cells are cultured in a cell culture dish containing complete growth media, wherein the complete growth media include ?-MEM, fetal bovine serum, and human-basic fibroblast growth factors. After mesenchymal stem cells are sub-cultured in the complete growth media for three times, a conditioned medium which can effectively increase the activation of tight junction protein Zonula occludens-1 (ZO-1) can be acquired from the basal medium.