Patents by Inventor Pei-Chung Hsieh

Pei-Chung Hsieh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Fusion polymerase and method for using the same
    Patent number: 11639498
    Abstract: This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.
    Type: Grant
    Filed: August 20, 2018
    Date of Patent: May 2, 2023
    Assignee: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, Jr., Theodore B. Davis, Andrew F. Gardner
  • Fusion Polymerase and Method for Using the Same
    Publication number: 20180355328
    Abstract: This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.
    Type: Application
    Filed: August 20, 2018
    Publication date: December 13, 2018
    Applicant: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, JR., Theodore B. Davis, Andrew F. Gardner
  • Fusion polymerase and method for using the same
    Patent number: 10081799
    Abstract: This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.
    Type: Grant
    Filed: February 14, 2017
    Date of Patent: September 25, 2018
    Assignee: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, Jr., Theodore B. Davis, Andrew Gardner
  • Fusion polymerase and method for using the same
    Patent number: 10041051
    Abstract: This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.
    Type: Grant
    Filed: February 14, 2017
    Date of Patent: August 7, 2018
    Assignee: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, Jr., Theodore B. Davis, Andrew Gardner
  • Synthon Formation
    Publication number: 20180216098
    Abstract: This disclosure provides, among other things, a composition comprising: a 5? exonuclease; a strand-displacing polymerase; and optionally a single strand DNA binding protein and/or a ligase. A method for polynucleotide assembly to form a synthon, as well as a kit for performing the same, are also described.
    Type: Application
    Filed: August 27, 2015
    Publication date: August 2, 2018
    Applicant: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, Jr., Theodore B. Davis, Andrew Gardner
  • Fusion polymerase and method for using the same
    Patent number: 9963687
    Abstract: This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: May 8, 2018
    Assignee: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, Jr., Theodore B. Davis, Andrew Gardner
  • Fusion Polymerase and Method for Using the Same
    Publication number: 20170152494
    Abstract: This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.
    Type: Application
    Filed: February 14, 2017
    Publication date: June 1, 2017
    Applicant: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, Jr., Theodore B. Davis, Andrew Gardner
  • Fusion Polymerase and Method for Using the Same
    Publication number: 20170152493
    Abstract: This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.
    Type: Application
    Filed: February 14, 2017
    Publication date: June 1, 2017
    Applicant: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, JR., Theodore B. Davis, Andrew Gardner
  • Synthon formation
    Patent number: 9447445
    Abstract: This disclosure provides, among other things, a composition comprising: a 5? exonuclease; a strand-displacing polymerase; and optionally a single strand DNA binding protein and/or a ligase. A method for polynucleotide assembly to form a synthon, as well as a kit for performing the same, are also described.
    Type: Grant
    Filed: August 27, 2015
    Date of Patent: September 20, 2016
    Assignee: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, Jr., Theodore B. Davis, Andrew Gardner
  • Fusion Polymerase and Method for Using the Same
    Publication number: 20160160193
    Abstract: This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.
    Type: Application
    Filed: February 17, 2016
    Publication date: June 9, 2016
    Applicant: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, JR., Theodore B. Davis, Andrew Gardner
  • Synthon Formation
    Publication number: 20160060671
    Abstract: This disclosure provides, among other things, a composition comprising: a 5? exonuclease; a strand-displacing polymerase; and optionally a single strand DNA binding protein and/or a ligase. A method for polynucleotide assembly to form a synthon, as well as a kit for performing the same, are also described.
    Type: Application
    Filed: August 27, 2015
    Publication date: March 3, 2016
    Applicant: NEW ENGLAND BIOLABS, INC.
    Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, JR., Theodore B. Davis, Andrew Gardner
  • Generation of random double-strand breaks in DNA using enzymes
    Patent number: 8703462
    Abstract: An enzyme preparation is described that includes a non-specific nuclease and a T7 Endo I mutant in a unit ratio of less than 1:200. This enzyme preparation may be used to generate double-stranded DNA fragments of a size suitable for DNA sequencing. The ends of the fragments can be readily modified as necessary to ligate adaptors or individual nucleotides to one strand of the double-stranded DNA fragments.
    Type: Grant
    Filed: February 3, 2010
    Date of Patent: April 22, 2014
    Assignee: New England Biolabs, Inc.
    Inventors: Pei-Chung Hsieh, Chudi Guan
  • Intracellular production of a nuclease
    Patent number: 8178335
    Abstract: Methods and compositions are provided that relate to obtaining a recombinant DNA and RNA cleaving nuclease. This involves the over-expression of a fusion protein between maltose-binding protein and a truncated nuclease in a soluble form in the cytoplasm of a host cell from which it can be readily extracted.
    Type: Grant
    Filed: March 28, 2011
    Date of Patent: May 15, 2012
    Assignee: New England Biolabs, Inc.
    Inventor: Pei-Chung Hsieh
  • Generation of Random Double-Strand Breaks in DNA Using Enzymes
    Publication number: 20110256593
    Abstract: An enzyme preparation is described that includes a non-specific nuclease and a T7 Endo I mutant in a unit ratio of less than 1:200. This enzyme preparation may be used to generate double-stranded DNA fragments of a size suitable for DNA sequencing. The ends of the fragments can be readily modified as necessary to ligate adaptors or individual nucleotides to one strand of the double-stranded DNA fragments.
    Type: Application
    Filed: February 3, 2010
    Publication date: October 20, 2011
    Applicant: NEW ENGLAND BIOLABS, INC.
    Inventors: Pei-Chung Hsieh, Chudi Guan
  • Intracellular Production of a Nuclease
    Publication number: 20110189753
    Abstract: Methods and compositions are provided that relate to obtaining a recombinant DNA and RNA cleaving nuclease. This involves the over-expression of a fusion protein between maltose-binding protein and a truncated nuclease in a soluble form in the cytoplasm of a host cell from which it can be readily extracted.
    Type: Application
    Filed: March 28, 2011
    Publication date: August 4, 2011
    Applicant: NEW ENGLAND BIOLABS, INC.
    Inventor: Pei-Chung Hsieh
  • Intracellular production of a nuclease
    Patent number: 7935509
    Abstract: Methods and compositions are provided that relate to obtaining a recombinant DNA and RNA cleaving nuclease. This involves the over-expression of a fusion protein between maltose-binding protein and a truncated nuclease in a soluble form in the cytoplasm of a host cell from which it can be readily extracted.
    Type: Grant
    Filed: August 12, 2005
    Date of Patent: May 3, 2011
    Assignee: New England Biolabs, Inc.
    Inventor: Pei-Chung Hsieh
  • Solubilization and purification of a target protein fused to a mutant maltose-binding protein
    Patent number: 7883867
    Abstract: Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein.
    Type: Grant
    Filed: August 16, 2010
    Date of Patent: February 8, 2011
    Assignee: New England Biolabs, Inc.
    Inventors: Paul Riggs, Pei-Chung Hsieh, Iris Walker, Paul A. Colussi, Mehul Ganatra, Christopher H. Taron
  • SOLUBILIZATION AND PURIFICATION OF A TARGET PROTEIN FUSED TO A MUTANT MALTOSE-BINDING PROTEIN
    Publication number: 20110020911
    Abstract: Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein.
    Type: Application
    Filed: August 16, 2010
    Publication date: January 27, 2011
    Applicant: New England Biolabs, Inc.
    Inventors: Paul Riggs, Pei-Chung Hsieh, Iris Walker, Paul A. Colussi, Mehul Ganatra, Christopher H. Taron
  • Solubilization and purification of a target protein fused to a mutant maltose-binding protein
    Patent number: 7825218
    Abstract: Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein.
    Type: Grant
    Filed: April 14, 2007
    Date of Patent: November 2, 2010
    Assignee: New England Biolabs, Inc.
    Inventors: Paul Riggs, Pei-Chung Hsieh, Iris Walker, Paul A. Colussi, Mehul Ganatra, Christopher H. Taron
  • Solubilization and Purification of a Target Protein fused to a Mutant Maltose-Binding Protein
    Publication number: 20090305342
    Abstract: Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein.
    Type: Application
    Filed: April 14, 2007
    Publication date: December 10, 2009
    Applicant: NEW ENGLAND BIOLABS, INC.
    Inventors: Paul Riggs, Pei-Chung Hsieh, Iris Walker, Paul A. Colussi, Mehul Ganatra, Christopher H. Taron