Abstract: The present invention discloses a method using gene detection to analyze personality traits of a human subject, which comprising steps of (a) providing a subject's test specimen; (b) conducting a gene polymorphism analysis for a plurality of genetic polymorphism biomarkers of the test specimen, wherein the plurality of genetic polymorphism biomarkers are composed of following single-nucleotide polymorphisms (SNPs); TPH1(SEQ ID NO:2), EGF(SEQ ID NO:4), NET_T-182C(SEQ ID NO:5), DRD1(SEQ ID NO:8), DRD4(SEQ ID NO:10) and COMT(SEQ ID NO:11), and following variable number tandem repeats (VNTRs): MAOA_VNTR(SEQ ID NO:16); and (c) determining the subject's surgerncy, negative affection and orienting/regulation of personality traits according to the results of the gene polymorphism analysis; wherein TPH1, DRD4 and COMT are correlated to surgency, EGF and DRD1, MAOA_VNTR are correlated to negative affection, and NET_T-182C and COMT are correlated to orienting/regulation of personality traits.