Abstract: Provided herein are compositions, systems, and methods comprising engineered effector proteins and uses thereof. These effector proteins may be characterized as CRISPR-associated (Cas) proteins. Various compositions, systems, and methods of the present disclosure may leverage the activities of these effector proteins for the modification, detection, and engineering of nucleic acids.

Abstract: Provided herein are compositions, systems, and methods comprising effector proteins and uses thereof. These effector proteins may be characterized as CRISPR-associated (Cas) proteins. Various compositions, systems, and methods of the present disclosure may leverage the activities of these effector proteins for the modification, detection, and engineering of nucleic acids.

Abstract: An array of hair dye products formulated to provide the same hair color to users with different hair colors or a different variety of colors of users with the same hair color. The array includes at least two hair dye products. The hair dye products each include a hair dye composition contained in a package. A first hair dye product in the array includes a photochromic dye that provides a color change to a first hair color, and a second hair dye product in the array includes the same photochromic dye that provides the same color change to a second, different color of hair. The array may also include two or more hair dye products that contain different ratios of photochromic dye(s) to provide users with different hair colors the same or different tunable hair colors.

Abstract: A tunable hair dye composition that includes a photochromic dye, a cosmetically acceptable solvent and, optionally, a carrier. When activated with a suitable activating electromagnetic radiation, the photochromic dye molecule changes color, thereby imparting a temporary color change to the composition and hair to which it is applied. When deactivated, the photochromic dye is generally colorless. The color imparted by the photochromic dye can be tuned by adjusting the wavelength and/or fluence of the activating and/or deactivating electromagnetic radiation. Thus, a wide variety of different hair colors can be obtained from a single hair dye composition containing specific combinations of photochromic dyes.

Abstract: Provided herein, in certain embodiments, are programmable nucleases, guide nucleic acids, and complexes thereof. Certain programmable nucleases provided herein comprise a RuvC domain. Also provided herein are nucleic acids encoding said programmable nucleases and guide nucleic acids. Also provided herein are methods of genome editing, methods of regulating gene expression, and methods of detecting nucleic acids with said programmable nucleases and guide nucleic acids.

Abstract: The present disclosure provides systems, methods, and compositions for conditionally regulating expression of a target gene. Aspects of the present disclosure utilize intracellular signal transduction pathways to regulate the expression of a gene (e.g., transgene, exogenous gene, endogenous gene).

Abstract: Provided herein are viral vectors comprising nucleotide sequences for production of an effector protein, guide nucleic acids for targeting modification of select genes to abrogate allogeneic immune reactions of T cells, and a donor nucleic acid encoding a chimeric antigen receptor (CAR), and uses thereof. Due to the small nature of the effector proteins provided herein, the viral vectors provided herein have ample room for all needed components for the efficient and robust production of CAR T cells from allogeneic donors. Various compositions, systems, and methods of the present disclosure leverage the activities of these effector proteins for the generation of “off-the-self” CAR T cells.

Abstract: Provided herein are compositions, systems, and methods comprising effector proteins and uses thereof. These effector proteins may be characterized as CRISPR-associated (Cas) proteins. Various compositions, systems, and methods of the present disclosure may leverage the activities of these effector proteins for the modification, detection, and engineering of nucleic acids, including editing target nucleic acids, as well as, the treatment of diseases and disorders associated with the dystrophin gene (DMD).

Abstract: An unlocking transmission mechanism is driven by a power transmission shaft of an electric control system to unlock an emergency exit door lock. The unlocking transmission mechanism includes a fixed base assembly, a slidable base assembly, a linear driving assembly, a transmission member and a linkage member. The transmission member is pivotally connected to the slidable base assembly, and the linkage member is pivotally connected to the transmission member. When the linear driving assembly is driven by the power transmission shaft to drive the slidable base assembly to move along a linear sliding stroke in an unlock-driving direction, the transmission member and the linkage member are sequentially driven to drive a manual-operated press bar assembly to unlock the emergency exit door lock. In addition, a multi-selective kit for the unlocking transmission mechanism is also provided.

Abstract: Provided herein are viral vectors comprising a transgene encoding a compact effector protein and uses thereof. These effector proteins are shown to be active with guide RNAs and may be characterized as CRISPR-associated (Cas) proteins. Due to the small nature of the effector protein, the viral vector has ample room for additional genome editing cargo, such as a guide nucleic acid and a donor nucleic acid. Various compositions, systems, and methods of the present disclosure leverage the activities of these effector proteins for the modification, detection, and engineering of nucleic acids.

Abstract: Disclosed herein, in certain embodiments, are methods of inducing cell cycle arrest, apoptosis, cell death, or a combination thereof, in a cell or population of cells. Also disclosed herein are methods of treating a disease or condition in an individual in need thereof comprising inducing cell cycle arrest, apoptosis, cell death, or a combination thereof in a population of cells in the individual. The cell or population of cells may comprise a nucleic acid sequence associated with a disease or condition, including an autoimmune disease, cancer, or an infectious disease. The methods described herein generally comprise contacting cells with a CRISPR-associated protein and a guide nucleic acid.

Abstract: Provided is an electrode mixture including: an electrode active material; an organic solvent; a binder; and an additive; wherein the binder contains a fluorine-containing copolymer containing a vinylidene fluoride unit and a fluorinated monomer unit, provided that the vinylidene fluoride unit is excluded from the fluorinated monomer unit; the additive is a polymer material containing the following repeating unit: —[CH2—CHR]— wherein R is a chain or cyclic amide group, a nitrile group, or a substituted alkyl group formed by substituting at least one of hydrogen atoms of an alkyl group having 1 to 4 carbon atoms with a chain or cyclic amide group or a nitrile group; a content of the binder is 0.1 to 1.6 parts by mass with respect to 100 parts by mass of the electrode active material; and a content of the additive is 0.001 to 0.2 parts by mass with respect to 100 parts by mass of the electrode active material.

Abstract: Provided herein, in certain embodiments, are programmable nucleases, guide nucleic acids, and complexes thereof. Certain programmable nucleases provided herein comprise a RuvC domain. Also provided herein are nucleic acids encoding said programmable nucleases and guide nucleic acids. Also provided herein are methods of genome editing, methods of regulating gene expression, and methods of detecting nucleic acids with said programmable nucleases and guide nucleic acids.

Abstract: A universal adaptor for door handle, operable to be universally assembled in a polygonal assembling groove of one of multiple types of handles, so that the above-mentioned handle forms a panel-like lock handle and is locked to a panel lock, and includes a lock portion and a universal extension portion. The lock portion is formed with at least one lock hole corresponding to the panel lock. The universal extension portion is connected to the lock portion and extends along an extension direction to form a polygonal rod structure, and a catching-type fit relationship is formed between the polygonal rod structure and the polygonal assembling groove. The present disclosure can achieve the functions and effects of universally assembling in polygonal assembling grooves of handles with different appearances and thereby enabling the handles to be locked to panel locks.

Abstract: Provided herein, in certain embodiments, are programmable nucleases, guide nucleic acids, and complexes thereof. Certain programmable nucleases provided herein comprise a RuvC domain. Also provided herein are nucleic acids encoding said programmable nucleases and guide nucleic acids. Also provided herein are methods of genome editing, methods of regulating gene expression, and methods of detecting nucleic acids with said programmable nucleases and guide nucleic acids.

Abstract: Methods for producing heterologous multi-subunit proteins in transformed cells are disclosed. In particular, the present disclosure provides improved methods of producing multi-subunit proteins, including antibodies and other multi-subunit proteins, which may or may not be secreted, with a higher yield and decreased production of undesired side-products. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.

Abstract: The present disclosure provides a system for regulating expression of a target polynucleotide in a cell. The system may comprise a chimeric polypeptide comprising a gene modulating polypeptide fused in-frame with a heterologous nuclear localization domain. The heterologous nuclear localization domain may be operable to translocate the chimeric polypeptide to a cell nucleus upon activation by an active cellular signaling pathway. The cellular signaling pathway may be inducible in response to an extracellular signal. In response to the extracellular signal, the chimeric polypeptide may localize to the cell nucleus and the gene modulating polypeptide may regulate expression of a target polynucleotide in the cell nucleus.

Abstract: The present disclosure provides systems, methods, and compositions for conditionally regulating expression of a target gene. Aspects of the present disclosure utilize intracellular signal transduction pathways to regulate the expression of a gene (e.g., transgene, exogenous gene, endogenous gene).

Abstract: Methods and compositions for treatment of a beta thalessemia are provided.

Abstract: Disclosed herein are methods and compositions for inactivating a glutamine synthetase (GS) gene, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.