Abstract: A cell activation reactor and a cell activation method are provided. The cell activation reactor includes a body, a rotating part, an upper cover, a microporous film, and multiple baffles. The body has an accommodating space, which is suitable for accommodating multiple cells and multiple magnetic beads. The rotating part is disposed in the accommodating space and includes multiple impellers. The microporous film is disposed in the accommodating space and covers multiple holes of the accommodating space. The baffles are disposed in the body. When the rotating part is driven to rotate, the interaction between the baffles and the impellers separates the cells and the magnetic beads.

Abstract: Methods of amplifying and determining a target nucleotide sequence are provided. The method of amplifying the target nucleotide sequence includes the following steps. A first adaptor and a second adaptor are linked to two ends of a double-stranded nucleic acid molecule with a target nucleotide sequence respectively to form a nucleic acid template, in which the first adaptor includes a Y-form adaptor or a hairpin adaptor and the second adaptor is a hairpin adaptor. Then, a PCR amplification cycle is performed on the nucleic acid template to obtain a PCR amplicon of the target nucleotide sequence.

Abstract: A cell culture device includes a culture unit, a gas supply unit, a first pressure unit, at least one inspecting unit and a control unit. The culture unit contains a cell culture liquid. The gas supply unit, connected with the culture unit, is used for transmitting a culture gas into the culture unit. The first pressure unit, connected with the culture unit, is used for applying a pressure to the cell culture liquid in the culture unit. The at least one inspecting unit, connected with the culture unit, is used for receiving the cell culture liquid for inspection. The control unit, electrically coupled with the culture unit, the first pressure unit, the gas supply unit and the at least one inspecting unit, is used for monitoring corresponding condition parameters to determine respective operations. In addition, a cell culture method for the cell culture device is also provided.

Abstract: A method for in vitro activation and/or expansion of immune cells is provided, including the steps of: a) providing magnetic particles having multi-protrusive surface modified with at least one type of immuno-inducing substance, in which each magnetic particle includes a copolymer core, a polymer layer, a magnetic substance layer, and a silicon-based layer from the inside to the outside; b) providing a cell solution including at least one type of immune cell in the cell solution; and c) bringing the magnetic particles in contact with the cell solution, in which the at least one type of immuno-inducing substance on the surface of the magnetic particle activates and/or expands the at least one type of immune cell in the cell solution.

Abstract: Described herein is a manufacturing method of a magnetic particle. First, deionized water, an organic solvent, a hydrophilic polymer, a lipid-soluble initiator, and at least two monomers are placed in a reactor and then stirred for polymerizing the at least two monomers into a copolymer to form a knobby copolymer core. Next, a polymer layer is formed to cover the knobby copolymer core, wherein the polymer layer has at least one functional group. Thereafter, a magnetic substance precursor is adsorbed by the knobby copolymer core covered with the polymer layer to form a magnetic substance layer. Further, a silicon-based layer may be additionally formed to cover the magnetic substance layer.

Abstract: An identification system of circulating biomarkers for cancer detection, a development method of circulating biomarkers for cancer detection, a cancer detection method and a kit are provided in the present disclosure, and the development method includes the following steps. Expression levels of multiple genes in normal tissue samples and tumor tissue samples are identified, and genes with high expression levels in the tumor tissue samples are selected. Afterwards, a weight of each human tissue’s contribution to plasma exosomes is calculated using tissue-specific genes and group-enriched genes. Next, expression levels of plasma exosome genes of healthy people and cancer patients are compared by an overlapping index, and circulating biomarkers and combinations thereof suitable for detection and evaluation of plasma exosomes are selected.

Abstract: A nucleic acid-drug complex is provided in the present disclosure, which includes a nucleic acid sequence of an anti-PD-L1 aptamer and a CpG oligonucleotide sequence capable of activating TLR9, in which the CpG oligonucleotide sequence consists of a first fragment and a second fragment, and the nucleic acid sequence of the anti-PD-L1 aptamer is inserted between the first fragment and the second fragment.

Abstract: A cell activation reactor and a cell activation method are provided. The cell activation reactor includes a body, a rotating part, an upper cover, a microporous film, and multiple baffles. The body has an accommodating space, which is suitable for accommodating multiple cells and multiple magnetic beads. The rotating part is disposed in the accommodating space and includes multiple impellers. The microporous film is disposed in the accommodating space and covers multiple holes of the accommodating space. The baffles are disposed in the body. When the rotating part is driven to rotate, the interaction between the baffles and the impellers separates the cells and the magnetic beads.

Abstract: A nucleic acid-drug complex is provided in the present disclosure, which includes a nucleic acid sequence of an anti-PD-L1 aptamer and a CpG oligonucleotide sequence capable of activating TLR9, in which the CpG oligonucleotide sequence consists of a first fragment and a second fragment, and the nucleic acid sequence of the anti-PD-L1 aptamer is inserted between the first fragment and the second fragment.

Abstract: Methods of amplifying and determining a target nucleotide sequence are provided. The method of amplifying the target nucleotide sequence includes the following steps. A first adaptor and a second adaptor are linked to two ends of a double-stranded nucleic acid molecule with a target nucleotide sequence respectively to form a nucleic acid template, in which the first adaptor includes a Y-form adaptor or a hairpin adaptor and the second adaptor is a hairpin adaptor. Then, a PCR amplification cycle is performed on the nucleic acid template to obtain a PCR amplicon of the target nucleotide sequence.

Abstract: A cell culture device includes a culture unit, a gas supply unit, a first pressure unit, at least one inspecting unit and a control unit. The culture unit contains a cell culture liquid. The gas supply unit, connected with the culture unit, is used for transmitting a culture gas into the culture unit. The first pressure unit, connected with the culture unit, is used for applying a pressure to the cell culture liquid in the culture unit. The at least one inspecting unit, connected with the culture unit, is used for receiving the cell culture liquid for inspection. The control unit, electrically coupled with the culture unit, the first pressure unit, the gas supply unit and the at least one inspecting unit, is used for monitoring corresponding condition parameters to determine respective operations. In addition, a cell culture method for the cell culture device is also provided.

Abstract: A biological sample processing device includes a base, a purification unit, a metering unit and a first tube. The purification unit is disposed on the base and is configured to purify a sample. The metering unit is disposed on the base and has an inlet, at least one metering trough and an overflow trough. The inlet is connected to the purification unit via the first tube, and the metering trough is connected between the inlet and the overflow trough. The sample from the purification unit is configured to enter the metering unit through the inlet to be moved toward the metering trough, and to be moved toward the overflow trough after the metering trough is filled with the sample.

Abstract: A test strip for microorganism detection includes: a porous substrate, a surface of which a surface has a first color; and a microorganism detection composition, which is at least adhered to a part of a surface of the porous substrate or at least adhered to a part of a surface of the porous substrate and a part of an interior of the porous substrate. The microorganism detection composition includes: a plurality of colored particles having a second color different than the first color; and a plurality of specific peptides immobilized on the surfaces of the plurality of colored particles, in which the plurality of specific peptides connect the plurality of colored particles to each other to make the plurality of colored particles aggregate to cover the part of the surface of the porous substrate and present the second color on the part of the surface of the porous substrate.

Abstract: A method for in vitro activation and/or expansion of immune cells is provided, including the steps of: a) providing magnetic particles having multi-protrusive surface modified with at least one type of immuno-inducing substance, in which each magnetic particle includes a copolymer core, a polymer layer, a magnetic substance layer, and a silicon-based layer from the inside to the outside; b) providing a cell solution including at least one type of immune cell in the cell solution; and c) bringing the magnetic particles in contact with the cell solution, in which the at least one type of immuno-inducing substance on the surface of the magnetic particle activates and/or expands the at least one type of immune cell in the cell solution.

Abstract: Described herein is a magnetic particle, which includes a knobby copolymer core, a polymer layer, a magnetic substance layer and a silicon-based layer. The polymer layer covers the knobby copolymer core and has at least one functional group. In addition, the magnetic substance layer covers the polymer layer and the silicon-based layer covers the magnetic substance layer. Furthermore, a manufacturing method of the magnetic particle is also described.

Abstract: A convective polymerase chain reaction apparatus includes a tube, a temperature control unit, at least one light source and a sensor. The tube includes a cavity used to contain a reaction solution. The reaction solution has a liquid level measured from a bottom of the cavity to a top surface of the reaction solution. The temperature control unit is disposed adjacent to the tube for controlling the temperature of the reaction solution. The at least one light source provides a light beam passing through an incident portion of the tube to excite the reaction solution emitting a fluorescent light. The incident portion is located at a height greater than ½ of a liquid level. The sensor is adjacent to the tube for detecting the excited fluorescence. The light beam has an incident direction forming a non-straight angle with a long axis of the tube.

Abstract: A biological sample processing device includes a base, a purification unit, a metering unit and a first tube. The purification unit is disposed on the base and is configured to purify a sample. The metering unit is disposed on the base and has an inlet, at least one metering trough and an overflow trough. The inlet is connected to the purification unit via the first tube, and the metering trough is connected between the inlet and the overflow trough. The sample from the purification unit is configured to enter the metering unit through the inlet to be moved toward the metering trough, and to be moved toward the overflow trough after the metering trough is filled with the sample.

Abstract: A convective polymerase chain reaction apparatus includes a tube, a temperature control unit, at least one light source and a sensor. The tube includes a cavity used to contain a reaction solution. The reaction solution has a liquid level measured from a bottom of the cavity to a top surface of the reaction solution. The temperature control unit is disposed adjacent to the tube for controlling the temperature of the reaction solution. The at least one light source provides a light beam passing through an incident portion of the tube to excite the reaction solution emitting a fluorescent light. The incident portion is located at a height greater than ½ of a liquid level. The sensor is adjacent to the tube for detecting the excited fluorescence. The light beam has an incident direction forming a non-straight angle with a long axis of the tube.

Abstract: A method for detecting metal ions and chemical/biochemical molecules is provided. The method includes providing a probe, wherein the probe includes: a gold nanocluster; a reducing agent and a chelating agent partially capped on a surface of the gold nanocluster, wherein the probe is formed of reducing gold ions by the reducing agent, and the gold ions and the reducing agent have a molar ratio of 1:0.7 to 1:1.9. The probe may interact with several metal ions of an aqueous solution to produce different changes of fluorescent spectra. Chemical/biochemical molecules can be detected by the fluorescent spectra difference caused by the interaction between the metal ions and the chemical/biochemical molecules.

Abstract: A gold nanocluster composition and method for preparing the same are provided. The method includes providing a gold ion-containing solution. Next, the method entails mixing the gold ion-containing solution and a reducing agent solution to obtain a first mixture liquid, and heating the first mixture liquid to obtain a second mixture liquid, wherein the second mixture liquid contains the gold nanoclusters, which are partially capped by reducing agent.