Abstract: A voltage convertor module includes a lead-frame, a unitary bare die and a molding body. The lead-frame can have a plurality of electrodes including an input voltage electrode, an output voltage electrode, a ground electrode and a controlling electrode. The unitary bare die is disposed only on the lead-frame, where a plurality of pads of the unitary bare die are electrically connected to the electrodes of the lead-frame correspondingly. The unitary bare die includes the plurality of pads, a buck controller block, a first switching unit block, a second switching unit block, a feedback unit block and a plurality of routing structures.

Abstract: A cell activation reactor and a cell activation method are provided. The cell activation reactor includes a body, a rotating part, an upper cover, a microporous film, and multiple baffles. The body has an accommodating space, which is suitable for accommodating multiple cells and multiple magnetic beads. The rotating part is disposed in the accommodating space and includes multiple impellers. The microporous film is disposed in the accommodating space and covers multiple holes of the accommodating space. The baffles are disposed in the body. When the rotating part is driven to rotate, the interaction between the baffles and the impellers separates the cells and the magnetic beads.

Abstract: The present invention relates to method of using a microfluidic chip for rapid nucleic acid hybridization, comprising: activating a porous substrate with positive charges; injecting a mixed solution of a test nucleic acid and a nucleic acid probe into the microfluidic chip for maintaining the test nucleic acid hybridized to the nucleic acid probe being absorbed to the periphery of the substrate; continuously washing the microfluidic chip with an anionic surfactant; and detecting the hybridization signals on the substrate after washing for a predetermined time; wherein the activation of the substrate with positive charges allows the test nucleic acid hybridized to the nucleic acid probe to form a micelle during washing and the diffusion of such from the periphery toward the center of the substrate to accelerate. Thus, it is possible to accomplish detection in a very short time for application of specific DNA complementary hybridization.

Abstract: The present invention relates to method of using a microfluidic chip for rapid nucleic acid hybridization, comprising: activating a porous substrate with positive charges; injecting a mixed solution of a test nucleic acid and a nucleic acid probe into the microfluidic chip for maintaining the test nucleic acid hybridized to the nucleic acid probe being absorbed to the periphery of the substrate; continuously washing the microfluidic chip with an anionic surfactant; and detecting the hybridization signals on the substrate after washing for a predetermined time; wherein the activation of the substrate with positive charges allows the test nucleic acid hybridized to the nucleic acid probe to form a micelle during washing and the diffusion of such from the periphery toward the center of the substrate to accelerate. Thus, it is possible to accomplish detection in a very short time for application of specific DNA complementary hybridization.

Abstract: A method for soft configuring communication a memory device to act as a Key Card that can switch on or off some particular functions of either an application program or the memory device itself. The method comprising the steps of: connecting the memory device to an external device; running a specific application program on the external device, the application program writing and storing a specific data into the memory device, the specific data including at least a command and a key; the memory device generating a response value according to the command and key of the specific data; and the external device reading the response value from the memory device and checking a status of the response value; if the response value is checked to be valid, then the application program will be able to access the memory device by using functions defined by the command; if the response value is checked to be invalid, then functions of the application program will be restricted.

Abstract: The present invention relates to a nucleic acid hybridization method and in particular to a method using a microfluidic chip-based hybridization device with modified procedures which shows the advantages of simplicity and efficiency. The method of the present invention is to mix the test nucleic acid and the probe nucleic acid in advance, feed the solution into a hybridization device with a porous substrate, let the nucleic acid to be analyzed absorb into the substrate. The washing solution is then injected into the same device to wash out the unhybridized probes and the signals of hybridization absorbed on the substrate are detected for analysis. The conventional 2-step approach for the hybridization of the nucleic acid to be analyzed and the probe is combined into one step by the method of the invention provided here, which simplifies the necessary steps and shorten the time needed for hybridization reaction and detection.

Abstract: The present invention relates to a biochip for nucleic acid hybridization. The biochip of the present invention comprises a hybridization chamber which is in the form of a cavity, a porous membrane pressed in the hybridization chamber; and at least one first circulation hole and at least one second circulation hole which are communicated with the hybridization chamber so that the reaction solution flows in the at least one first circulation hole and flows out the at least one second circulation hole through the pores of the porous membrane. The hybridization reaction area is increased by flowing the reaction solution through the pores of the membrane, which enable the reaction sensitivity to be increased. The diffusion distance for the reaction molecules is decreased due to the limited inside space of the membrane, and thereby the hybridization time is shortened.

Abstract: A method is used for operating a memory card, which comprises following steps: (1) a file is preloaded in the first sector of the file allocation table and the second sectors of the description block, wherein the file at least including first data, second data and third data, wherein the first sector is filled up with the first data, only one of the second sectors is not filled up with the second data and the other second sectors are filled up with the third data. (2) A command is received, where the command is capable of updating the file. (3) The command for the first sector is ignored. (4) The only one of the second sectors is updated according to the command. (5) The command for the other second sectors is ignored. (6) The second data of the only one of the second sectors are recovered.

Abstract: A memory device preloads a command file and a plurality of response files. Whenever a host sends a command to the memory apparatus, the command assigns one of the response files; thereby the host can receive response of the memory apparatus by reading the assigned response file.

Abstract: The present invention relates to a blotting method for rapidly analyzing nucleic acid comprising the steps of transferring a nucleic acid to be analyzed to the substrate and fixing the nucleic acid to be analyzed absorbed on the substrate; directing adding a nucleic acid probe to hybridize in a short time, without blocking the areas where the nucleic acid to be analyzed has not been fixed; removing the nucleic acid probe which has not been annealed to the nucleic acid to be analyzed by washing; and finally detecting the hybridization signal. According to the present invention, since the prehybridization is not needed and the hybridization and washing time is shortened, the time for the nucleic acid hybridization is dramatically shortened. Therefore, the whole blotting procedures for rapidly analyzing nucleic acid may be finished quickly.

Abstract: The present invention relates to a hybridization device coupled by means of vibration to shorten the time needed for hybridization. This hybridization device of the present invention comprises an oscillation generating unit; a spacer attached to the top of the oscillation generating unit, which wraps around to form a tank, and the tank is loaded with a transmission solution; a loading plate having a loading face was paved with at least one nucleic acid probe, and the flip side of the loading face is a transmission face, which covers on top of the spacer to form a closed space, and is attached with the transmission solution. The vibration generated by the oscillation generating unit can be propagated to the loading plate through the transmission solution to promote the effective mixing of the nucleic acid to be analyzed and the probe, further to shorten the time needed for nucleic acid hybridization and to increase the detection sensitivity.

Abstract: A method for soft configuring communication a memory device to act as a Key Card that can switch on or off some particular functions of either an application program or the memory device itself. The method comprising the steps of: connecting the memory device to an external device; running a specific application program on the external device, the application program writing and storing a specific data into the memory device, the specific data including at least a command and a key; the memory device generating a response value according to the command and key of the specific data; and the external device reading the response value from the memory device and checking a status of the response value; if the response value is checked to be valid, then the application program will be able to access the memory device by using functions defined by the command; if the response value is checked to be invalid, then functions of the application program will be restricted.

Abstract: The present invention relates to a biochip for nucleic acid hybridization. The biochip of the present invention comprises a hybridization chamber which is in the form of a cavity, a porous matrix pressed in the hybridization chamber; and at least one first circulation hole and at least one second circulation hole which are communicated with the hybridization chamber so that the reaction solution flows in the at least one first circulation hole and flows out the at least one second circulation hole through the pores of the porous matrix. The hybridization reaction area is increased by flowing the reaction solution through the pores of the membrane, which enable the reaction sensitivity to be increased. The diffusion distance for the reaction molecules is decreased due to the limited inside space of the membrane, and thereby the hybridization time is shortened.

Abstract: The present invention relates to a nucleic acid hybridization method and in particular to a method using a microfluidic chip-based hybridization device with modified procedures which shows the advantages of simplicity and efficiency. The method of the present invention is to mix the test nucleic acid and the probe nucleic acid in advance, feed the solution into a hybridization device with a porous substrate, let the nucleic acid to be analyzed absorb into the substrate. The washing solution is then injected into the same device to wash out the unhybridized probes and the signals of hybridization absorbed on the substrate are detected for analysis. The conventional 2-step approach for the hybridization of the nucleic acid to be analyzed and the probe is combined into one step by the method of the invention provided here, which simplifies the necessary steps and shorten the time needed for hybridization reaction and detection.

Abstract: The present invention relates to a blotting method for rapidly analyzing nucleic acid comprising the steps of transferring a nucleic acid to be analyzed to the substrate and fixing the nucleic acid to be analyzed absorbed on the substrate; directing adding a nucleic acid probe to hybridize in a short time, without blocking the areas where the nucleic acid to be analyzed has not been fixed; removing the nucleic acid probe which has not been annealed to the nucleic acid to be analyzed by washing; and finally detecting the hybridization signal. According to the present invention, since the prehybridization is not needed and the hybridization and washing time is shortened, the time for the nucleic acid hybridization is dramatically shortened. Therefore, the whole blotting procedures for rapidly analyzing nucleic acid may be finished quickly.