Abstract: The present disclosure relates, in part, to improved methods of making single-stranded DNA (ssDNA) from double-stranded DNA (dsDNA), as well as use of the resulting ssDNA for genome engineering. The disclosure also relates, in part, to improved methods of genetic modification using single stranded DNA binding proteins.

Abstract: A wet-dry vacuum cleaner device comprising: a housing; a motor-fan assembly mounted in the housing and arranged to generate an air flow between a dirty air inlet and a clean air outlet; a tank arranged to capture liquid entrained in the dirty air flow, the tank comprising a tank inlet in fluid communication with the dirty air inlet and a tank outlet in fluid communication with the motor fan assembly; wherein the tank outlet is in fluid communication with a liquid trap mounted inside the tank arranged to capture liquid entrained in the air flow exiting the tank.

Abstract: Provided are methods, compositions, reagents, kits that are useful for detecting a specific nucleic acid region in genome with high efficiency and high sensitivity.

Abstract: Provided are methods, compositions, reagents, kits that are useful for detecting a specific nucleic acid region in genome with high efficiency and high sensitivity.

Abstract: Systems and methods for providing therapeutic shock waves are provided. A modified acoustic lens can include a first lens portion for directing a first part of an acoustic shock wave pulse toward a target and a second lens portion for directing a second part of the acoustic shock wave pulse toward the target. The second lens portion can be offset relative to the first portion such that transmission of the second part of the acoustic shock wave pulse through the second lens portion is delayed by a predetermined amount relative to transmission of the first part through the first lens portion. In situ superposition of the first and second pulses near and at the target can lead to the formation of a pressure waveform with idealized pulse profile and broadened focal width, which can provide for improved comminution of a concretion located within a living body with reduced tissue injury.

Abstract: Provided are methods, compositions, reagents, kits that are useful for detecting a specific nucleic acid region in genome with high efficiency and high sensitivity.

Abstract: A sander may include a housing, a motor, a sanding platen, and a two-piece fan. The motor may be disposed within the housing and may include an output shaft. The sanding platen may be driven by the motor for movement relative to the housing. The fan may be driven by the motor and may include a first piece formed from a first material and a second piece formed from a second material. The first piece may include a disk-shaped body having first and second opposite sides. The first side may include a plurality of first blades extending therefrom. The second side may include a plurality of second blades extending therefrom and a recess formed therein. The second piece may be received in the recess and may include a counterweight boss formed thereon.

Abstract: Lithotripter apparatuses and methods are provided for selectively modifying an acoustic pressure field and can include a shock wave source operable to generate a shock wave having a substantially axisymmetric acoustic pressure field, an acoustic focusing member positioned between the shock wave source and a target, and an acoustic barrier positioned between the shock wave source and the acoustic focusing member. The acoustic barrier can be operable to selectively block a portion of the shock wave generated by the shock wave source such that the substantially axisymmetric pressure field is transformed into a modified acoustic pressure field.

Abstract: A bun machine includes: a pressing device for forming a first layer and a second layer; an overlaying device for forming a bun crust with the first layer and the second layer; a main transporter, wherein a first end of the main transporter is connected to the overlaying device for transporting the bun crust; a feeding device provided aside the main transporter, comprising: a filling tank for storing and inputting fillings into the feeding device; and a feeding unit feeding the fillings onto the bun crust; a wrapping device provided above the main transporter, wherein the wrapping device wraps the fillings with the bun crust for forming a filled roll; and a separating device connected to a second end of the main transporter, wherein the separating device squeezes the filled roll for separating and sealing the filled roll, so as to produce buns.

Abstract: A sander may include a housing, a motor, a sanding platen, and a two-piece fan. The motor may be disposed within the housing and may include an output shaft. The sanding platen may be driven by the motor for movement relative to the housing. The fan may be driven by the motor and may include a first piece formed from a first material and a second piece formed from a second material. The first piece may include a disk-shaped body having first and second opposite sides. The first side may include a plurality of first blades extending therefrom. The second side may include a plurality of second blades extending therefrom and a recess formed therein. The second piece may be received in the recess and may include a counterweight boss formed thereon.

Abstract: A sander may include a housing, a motor, a sanding platen, and a two-piece fan. The motor may be disposed within the housing and may include an output shaft. The sanding platen may be driven by the motor for movement relative to the housing. The fan may be driven by the motor and may include a first piece formed from a first material and a second piece formed from a second material. The first piece may include a disk-shaped body having first and second opposite sides. The first side may include a plurality of first blades extending therefrom. The second side may include a plurality of second blades extending therefrom and a recess formed therein. The second piece may be received in the recess and may include a counterweight boss formed thereon.

Abstract: A sander may include a housing, a motor, a sanding platen, and a two-piece fan. The motor may be disposed within the housing and may include an output shaft. The sanding platen may be driven by the motor for movement relative to the housing. The fan may be driven by the motor and may include a first piece formed from a first material and a second piece formed from a second material. The first piece may include a disk-shaped body having first and second opposite sides. The first side may include a plurality of first blades extending therefrom. The second side may include a plurality of second blades extending therefrom and a recess formed therein. The second piece may be received in the recess and may include a counterweight boss formed thereon.

Abstract: Lithotripter apparatuses and methods are provided for selectively modifying an acoustic pressure field and can include a shock wave source operable to generate a shock wave having a substantially axisymmetric acoustic pressure field, an acoustic focusing member positioned between the shock wave source and a target, and an acoustic barrier positioned between the shock wave source and the acoustic focusing member. The acoustic barrier can be operable to selectively block a portion of the shock wave generated by the shock wave source such that the substantially axisymmetric pressure field is transformed into a modified acoustic pressure field.

Abstract: Systems and methods for providing therapeutic shock waves are provided. A modified acoustic lens can include a first lens portion for directing a first part of an acoustic shock wave pulse toward a target and a second lens portion for directing a second part of the acoustic shock wave pulse toward the target. The second lens portion can be offset relative to the first portion such that transmission of the second part of the acoustic shock wave pulse through the second lens portion is delayed by a predetermined amount relative to transmission of the first part through the first lens portion. In situ superposition of the first and second pulses near and at the target can lead to the formation of a pressure waveform with idealized pulse profile and broadened focal width, which can provide for improved comminution of a concretion located within a living body with reduced tissue injury.

Abstract: Compositions and methods are provided for generating three frame cDNA expression libraries for functional screening of proteins. The invention includes sets of 5? adapters for cloning cDNA molecules in which the sets include three adapters that can be used to clone a particular cDNA in all three reading frames. The libraries so generated have greater complexity of expressed open reading frames, and thus can improve the success of functional protein screens, such as two hybrid screens. The adapters also have recombination sites for efficient cloning and transfer between systems.

Abstract: The invention relates to an improved apparatus for the comminution of concretions in vivo by controlled, concentrated cavitation energy using two shock wave pulses with a specified time delay and pressure relationship, with the first shock wave pulse being used to induce a transient cavitation bubble cluster near the target concretion, and the second shock wave pulse to control and force the collapse of the cavitation bubble cluster towards the target concretion with concentrated energy disposition while avoiding injury to surrounding tissue. The invention contemplates the use of an improved combined electrohydraulic or electromagnetic and a piezoelectric annular array shock wave generator to produce improved stone comminution with reduced tissue injury in vivo.

Abstract: A method to reduce vascular injury produced by lithotripter shock waves comprising the use of particular multi-pulsed shock waves which comminute human concretions with reduced vascular damage.

Abstract: A method to reduce vascular injury produced by lithotripter shock waves comprising the use of particular multi-pulsed shock waves which comminute human concretions with reduced vascular damage.

Abstract: This invention discloses an apparatus and method for producing microcavitational activity in aqueous fluids for non-invasive macromolecule delivery into living cells. A standard electrohydraulic shock wave lithotripter is fitted with an adjustable ring reflector that shares the same foci as the standard lithotripter hemi-ellipsoidal reflector. A small portion of the spherical shock wave, generated by the spark discharge at the first focus (F1), is reflected and diffracted by the ring reflector, resulting in a weak preceding shock wave approximately 8.5 &mgr;s in front of the lithotripter shock wave reflected and diffracted by the hemi-ellipsoidal reflector. The peak negative pressure of the preceding weak shock wave or pulse at F2 can be adjusted from −0.96 to −1.91 MPa, using an output voltage of 25 kV. Living cells are exposed to the preceding shock wave and the lithotripter shock wave.

Abstract: The invention discloses a microsecond tandem-pulse electrohydraulic shock wave generator for the comminution of concretions in vivo by controlled, concentrated cavitation energy. This apparatus generates a primary and a secondary shock wave pulse with a specified time delay in microseconds and pressure relationships, with the primary shock wave pulse being used to induce a transient cavitation bubble cluster near the target concretion, and the secondary shock wave pulse consisting of a sequence of reflected pulses producing a pulse that is substantially compressive to control and force the collapse of the cavitation bubble cluster towards the target concretion. This microsecond tandem-pulse is produced by the use of a plurality of ellipsoidal reflecting surfaces of the same focal length, but different major and minor axes.