Abstract: Disclosed are a flexible molecularly imprinted sensor for detecting zeatin riboside, a preparation method and use thereof. The preparation method includes preparing a laser-induced graphene pattern on a polyimide tape by a laser direct writing technology, and transferring the laser-induced graphene pattern onto polydimethylsiloxane, then modifying a composite material of zinc-aluminum layered double hydroxide and an iron-based metal-organic framework onto a working electrode, and then preparing a molecularly imprinted film on a surface of an electrode using o-phenylenediamine as a monomer and zeatin riboside as a template molecule, and eluting the template molecule to obtain the flexible molecularly imprinted sensor.