Abstract: A time-resolved fluorescence immunochromatography kit for the simultaneous detection of a mixed pollutant of aflatoxin and carbaryl, a preparation method therefor, and an application thereof. The kit comprises a time-resolved fluorescence immunochromatography test strip and a sample reaction vial containing a europium-labeled anti-aflatoxin monoclonal antibody and a europium-labeled anti-carbaryl monoclonal antibody lyophilized product, detection lines of the time-resolved fluorescence immunochromatography test strip being coated with an aflatoxin-bovine serum albumin conjugate and a carbaryl-ovalbumin conjugate respectively, and the anti-carbaryl monoclonal antibody is produced by secretion of a hybridoma cell line Jnw1D2 with a preservation number of CCTCC NO. C201654. The kit can be used for the simultaneous detection of aflatoxin and carbaryl content in a sample, and features simple and quick operations and a high sensitivity.

Abstract: A time-resolved fluorescence immunochromatographic kit for simultaneous detection of mixed contamination of five mycotoxins such as aflatoxin B1 and an application thereof are disclosed. The kit comprises a time-resolved fluorescent immunochromatographic test strip and sample reaction vials each containing a europium-labeled monoclonal antibody lyophilized product; wherein the fluorescent test strip comprises a PVC substrate, and a surface of the PVC substrate is adhered with a water absorbing pad (1), a detection pad (2) and a sample pad (3) from top to bottom, adjacent pads being connected and overlapping at connections. The detection pad (2) adopts a nitrocellulose membrane as the base thereof and is arranged with a lateral quality control line (5) and five detection lines (5, 6, 7, 8, 9) from top to bottom each covered by a bovine serum albumin conjugate of each toxin.

Abstract: The present invention relates to an indicator molecule for the toxigenicity of aflatoxigenic fungi and the use thereof. The amino acid sequence of the indicator molecule ABFT-YJFZ01 for the toxigenicity of the aflatoxigenic fungi is as represented in SEQ ID NO. 1. The indicator molecule is used for identifying the aflatoxin-producing capacity of the Aspergillus aflatoxigenic fungi and identifying whether the aflatoxigenic fungi with high toxigenicity is present in farmland, agricultural products and feed, and is easy to popularize and apply.

Abstract: The present disclosure belongs to the technical field of biosensors and particularly provides a construction method for a photocathode indirect competition sensor and an evaluation method. The construction method includes: using Z-type Bi2O3/CuBi2O4 as a sensing platform; calculating a photoinduced electron Z-type transfer path and an energy band structure of Bi2O3 and CuBi2O4 using a density functional theory (DFT); and constructing a Bi2O3/CuBi2O4-based biosensor. A photoelectrochemical (PEC) photocathode biosensor based on a Bi2O3/CuBi2O4 heterojunction prepared through the solution has good repeatability, reproducibility, stability, and specificity for detecting a target. The PEC biosensor constructed in the solution of the present disclosure has a broad application prospect in the fields of healthcare, environment, and food.

Abstract: The present disclosure belongs to the field of microbial technology, and in particular relates to a microbial agent for promoting root nodule number increase and root nodule nitrogenase activity increase in leguminous crops and use thereof. The microbial agent is compounded from 4 microbes including Bacillus amyloliquefaciens, Brevibacillus laterosporu, Bacillus mucilaginosus Krassilnikov, and Enterobacter ludwigii by separate fermentative cultivation, concentration and mixing. The microbial agent can effectively promote root nodule number increase and root nodule nitrogenase activity increase, promote crop growth, and improve crop yield and product quality.

Abstract: The present disclosure relates to a microbial agent with functions of preventing and controlling aflatoxin and aflatoxigenic strain thereof and promoting yield increase of crops. The microbial agent is compounded from 5 microbes comprising Bacillus amyloliquefaciens, Brevibacillus laterosporu, Bacillus mucilaginosus Krassilnikov, Enterobacter ludwigii and Myroides odoratimimus by separate fermentative cultivation, concentration and mixing. The microbial agent is applied to a field planting stage of crops such as peanuts, which can effectively reduce the abundance and infection probability of toxin-producing strain such as Aspergillus flavus in soil from the source, reduce the risk of aflatoxin pollution of peanuts after production, improve the quality and safety level of peanuts, and at the same time, promote crop growth, enhance the resistance, and improve the full pod rate and yield, and has significant economic, social and ecological benefits.

Abstract: The present invention relates to an early warning method before the occurrence of aflatoxin contamination.

Abstract: A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing europium-labeled anti-4,15-diacetoxyscirpenol, anti-aflatoxin B1 and anti-sterigmatocystin monoclonal antibodies, where a water absorption pad, a detection pad and a sample pad are sequentially disposed on one side of the immunochromatography time-resolved fluorescence test strip from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and detection lines are transversely arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, and the three detection lines each are coated with a toxin-protein conjugate.

Abstract: A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol and T-2 toxin. The kit includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing freeze-dried products of europium-labeled monoclonal antibodies of toxins, where the immunochromatography time-resolved fluorescence test strip includes a liner, where a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a transverse quality control line and detection lines are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the quality control line, and the detection lines each are coated with a toxin-protein conjugate.

Abstract: The present disclosure discloses an intelligent point-of-care testing device for mycotoxins based on a quantum dots test strip. A first light source and a second light source are used for exciting a test strip which is placed in a shell and coated with a mycotoxin antigen to obtain emitted light, and a light filtering component is used for filtering out impurity light. A clamping mechanism is used for clamping and fixing an image capturing component. The image capturing component is used for capturing, an image of mycotoxins based on a quantum dots test strip. A processor is used for analyzing and calculating image information to obtain a mycotoxin content result. The testing time is shortened, and the portability and practicability of mycotoxin testing are improved. Quantitative instant testing of single or multi-toxin mixed pollution is achieved, and the technical problem of fluorescence quantification is solved.

Abstract: The present disclosure belongs to the technical field of biosensors and particularly provides a construction method for a photocathode indirect competition sensor and an evaluation method. The construction method includes: using Z-type Bi2O3/CuBi2O4 as a sensing platform; calculating a photoinduced electron Z-type transfer path and an energy band structure of Bi2O3 and CuBi2O4 using a density functional theory (DFT); and constructing a Bi2O3/CuBi2O4-based biosensor. A photoelectrochemical (PEC) photocathode biosensor based on a Bi2O3/CuBi2O4 heterojunction prepared through the solution has good repeatability, reproducibility, stability, and specificity for detecting a target. The PEC biosensor constructed in the solution of the present disclosure has a broad application prospect in the fields of healthcare, environment, and food.

Abstract: An immunoadsorbent and a composite affinity column for purifying fumonisin B1, anguidin, T-2 toxin, zearalenone, and vomitoxin. The immunoadsorbent includes a solid phase carrier, and a fumonisin B1 monoclonal antibody, an anguidin monoclonal antibody, a T-2 toxin monoclonal antibody, a zearalenone monoclonal antibody and a vomitoxin monoclonal antibody which are coupled to the solid phase carrier, the anguidin monoclonal antibody is a monoclonal antibody secreted by a hybridoma cell strain DAS5G11E7 having an accession number of CCTCCNO:C201881. The affinity column can be used for high performance liquid chromatography-mass spectrometry detection of the fumonisin B1, the anguidin, the T-2 toxin, the zearalenone and the vomitoxin, and has stable performance. Furthermore, an economical, quick, precise and safe detection method is established of the basis of the affinity column, and can be used for purifying and detecting samples of the five toxins without mutual interference and influence.

Abstract: The present disclosure discloses an intelligent point-of-care testing device for mycotoxins based on a quantum dots test strip. A first light source and a second light source are used for exciting a test strip which is placed in a shell and coated with a mycotoxin antigen to obtain emitted light, and a light filtering component is used for filtering out impurity light. A clamping mechanism is used for clamping and fixing an image capturing component. The image capturing component is used for capturing, an image of mycotoxins based on a quantum dots test strip. A processor is used for analyzing and calculating image information to obtain a mycotoxin content result. The testing time is shortened, and the portability and practicability of mycotoxin testing are improved. Quantitative instant testing of single or multi-toxin mixed pollution is achieved, and the technical problem of fluorescence quantification is solved.

Abstract: An immunochromatography test strip for detecting pollution of diacetoxyscirpenol includes a bottom plate, where a water absorption pad, a detection pad, a gold-labeled pad and a sample pad are sequentially attached to one side of the bottom plate from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and a detection line are transversely arranged on the nitrocellulose membrane, the quality control line is coated with a rabbit antimouse polyclonal antibody, the detection line is located below the quality control line, and the detection line is coated with a diacetoxyscirpenol-ovalbumin conjugate; the gold-labeled pad is transversely spray-coated with a nanogold-labeled anti-diacetoxyscirpenol monoclonal antibody; and the anti-diacetoxyscirpenol monoclonal antibody is secreted by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO.C201881.

Abstract: The invention belongs to the field of microbes and specifically relates to a myroides odoratimimus biocontrol strain for efficiently degrading aflatoxin and an application thereof. A myroides odoratimimus biocontrol strain 3J2MO is preserved at the China Center for Type Culture Collection (referred to as CCTCC) on Jun. 13, 2017 with preservation number CCTCC No. M 2017329. The myroides odoratimimus biocontrol strain of the invention can effectively degrade aflatoxin and may be configured to degrade aflatoxin and treat aflatoxin pollution of food crops.

Abstract: A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, aflatoxin B1 and sterigmatocystin includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing europium-labeled anti-4,15-diacetoxyscirpenol, anti-aflatoxin B1 and anti-sterigmatocystin monoclonal antibodies, where a water absorption pad, a detection pad and a sample pad are sequentially disposed on one side of the immunochromatography time-resolved fluorescence test strip from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and detection lines are transversely arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, and the three detection lines each are coated with a toxin-protein conjugate.

Abstract: A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol and T-2 toxin. The kit includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing freeze-dried products of europium-labeled monoclonal antibodies of toxins, where the immunochromatography time-resolved fluorescence test strip includes a liner, where a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a transverse quality control line and detection lines are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the quality control line, and the detection lines each are coated with a toxin-protein conjugate.

Abstract: An immunochromatography test strip for detecting pollution of diacetoxyscirpenol includes a bottom plate, where a water absorption pad, a detection pad, a gold-labeled pad and a sample pad are sequentially attached to one side of the bottom plate from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a quality control line and a detection line are transversely arranged on the nitrocellulose membrane, the quality control line is coated with a rabbit antimouse polyclonal antibody, the detection line is located below the quality control line, and the detection line is coated with a diacetoxyscirpenol-ovalbumin conjugate; the gold-labeled pad is transversely spray-coated with a nanogold-labeled anti-diacetoxyscirpenol monoclonal antibody; and the anti-diacetoxyscirpenol monoclonal antibody is secreted by a hybridoma cell strain DAS5G11E7 with the preservation number of CCTCC NO.C201881.

Abstract: The present invention relates to an early warning method before the occurrence of aflatoxin contamination.

Abstract: The present invention relates to an aflatoxin contamination risk warning molecule and use thereof. The steps are as follows: weighing a quantitative sample, extracting the aflatoxin contamination risk warning molecule to obtain a sample extract, and detecting and analyzing the sample extract to obtain a quantitative result of the aflatoxin contamination risk warning molecule; performing modeling with a chemometrics method using the content of one or more of the aflatoxin contamination risk warning molecules as a variable to obtain a classification prediction model, and performing risk assessment on aflatoxin contamination risk of the sample based on the classification prediction model, wherein a warning molecule of an aflatoxin toxigenic strain is one or a combination of more than one of versiconol (VOH), versicolorin B (Ver B), and 5-methoxysterigmatocystin (5-MST).