Abstract: A cross primer isothermal amplification primer set for detecting tobacco ringspot virus, a kit and application thereof are provided. The kit and the method provided by the disclosure have good specificity and negative reaction to quarantine viruses such as southern bean mosaic virus, tomato ringspot virus, Arabidopsis mosaic virus, bean pod mottle virus and the like. The real-time fluorescence detection method has high sensitivity and can detect the RNA template of the tobacco ringspot virus with the minimum concentration of 5 ng/?L. The lowest detectable concentration of RNA template was 0.5 ng/?L. It is very suitable for on-site detection of medical and health, food safety and import and export quarantine, and is easy to be popularized and applied in a wide range.

Abstract: A TPR1 gene related to a low-temperature tolerance of Pomacea, coding protein and application of the gene is disclosed. The disclosure belongs to the field of biotechnology. The present disclosure is rapid, effective and reproducible, and is an important complement to the TPR1 gene family. Pomacea is an important invasive organism, the disclosure is helpful for developing novel biological pesticide, and the low-temperature tolerance of the organism is reduced by blocking the expression of TPR1 gene in Pomacea, so as to control the further northward invasion of Pomacea. Meanwhile, by interfering with the expression of TPR1 gene, the hatching rate of Pomacea eggs can be significantly reduced, and the hatching period of eggs can be prolonged, thereby reducing the harm of Pomacea invasion.

Abstract: A compound plant-derived molluscicide for controlling Pomacea canaliculata and a preparation method and application thereof are disclosed. The compound plant-derived molluscicide is prepared from following raw materials in proportion by weight: 40-45% of water extract of Solidago canadensis L. leaves, 45-50% of water extract of Lantana camara L. roots, 0.1-0.2% of active ingredient stabilizer, and 0.1-0.2% of preservative; and a balance is ultrapure water. The disclosure has rich raw material resources, low cost, good molluscicidal effect, sustained and stable efficacy, non-toxic effect on freshwater fish, and meets the requirements of environmental protection.