Abstract: A direct structured illumination microscopy (dSIM) reconstruction method is provided. First, a time domain modulation signal is extracted through a wavelet. Then, an incoherent signal is converted into a coherent signal. Next, an accumulation amount at each pixel is calculated. Finally, a super-resolution image is generated by using a correlation between signals at different spatial positions. An autocorrelation algorithm of dSIM is insensitive to an error of a reconstruction parameter. dSIM bypasses a complex frequency domain operation in structured illumination microscopy (SIM) image reconstruction, and prevents an artifact caused by the parameter error in the frequency domain operation. The dSIM algorithm has high adaptability and can be used in laboratory SIM, nonlinear SIM imaging systems, or commercial systems.

Abstract: A direct structured illumination microscopy (dSIM) reconstruction method is provided. First, a time domain modulation signal is extracted through a wavelet. Then, an incoherent signal is converted into a coherent signal. Next, an accumulation amount at each pixel is calculated. Finally, a super-resolution image is generated by using a correlation between signals at different spatial positions. An autocorrelation algorithm of dSIM is insensitive to an error of a reconstruction parameter. dSIM bypasses a complex frequency domain operation in structured illumination microscopy (SIM) image reconstruction, and prevents an artifact caused by the parameter error in the frequency domain operation. The dSIM algorithm has high adaptability and can be used in laboratory SIM, nonlinear SIM imaging systems, or commercial systems.

Abstract: A system for microscopy comprising a reflector for a sample to be disposed adjacent thereto. The system comprises a radiation source for generating electromagnetic radiation and arranged for the electromagnetic radiation when so generated to pass at least partially through the sample when so disposed. When the electromagnetic radiation is so generated, interference is localized at least partially within the sample when so positioned, the interference being electromagnetic interference between the electromagnetic radiation and a reflected electromagnetic radiation that is at least part of the electromagnetic radiation reflected from the reflector.

Abstract: A stimulated-emission-depletion (STED) super-resolution microscope includes an excitation light source, a depletion light source, an excitation light expanded beam alignment system, a spiral-shaped phase plate, a Bessel beam generating system, a depletion light focus lens, a beam combination system, an objective lens, a piezoelectric scanning system, a filter, a signal collection system, and a single-photon detector. The depletion light can be a first-order Bessel beam. The depletion light has anti-scattering and self-healing characteristics, and is capable of keeping the spot shape at a deeper position of a sample, thereby improving image resolution in the deep region of the sample. Compared to conventional STED super resolution microscope of deep-layer imaging using an adjustable correction collar, the present invention is simpler in experimental operations and does not require active adjustments. Compared to adaptive optical systems, the present experimental apparatus is simpler and less expensive.

Abstract: “A stimulated-emission-depletion (STED) super-resolution microscope includes an excitation light source, a depletion light source , an excitation light expanded beam alignment system, a spiral-shaped phase plate, a Bessel beam generating system, a depletion light focus lens, a beam combination system , an objective lens, a piezoelectric scanning system, a filter, a signal collection system, and a single-photon detector. The depletion light can be a first-order Bessel beam. The depletion light has anti-scattering and self-healing characteristics, and is capable of keeping the spot shape at a deeper position of a sample, thereby improving image resolution in the deep region of the sample. Compared to conventional STED super resolution microscope of deep-layer imaging using an adjustable correction collar, the present invention is simpler in experimental operations and does not require active adjustments. Compared to adaptive optical systems, the present experimental apparatus is simpler and less expensive.

Abstract: An endoscopic imaging device for endoscopy in a body vessel is disclosed. The device comprises an annular illumination tube comprising an annular illumination fiber for illuminating a body vessel during endoscopy. The device further includes a first imaging tube comprising a first imaging fiber for gross examination and navigation through the body vessel. The first imaging fiber is disposed within the annular illumination tube. The device further comprises a second imaging tube comprising a second imaging fiber for cellular imaging. The second illumination fiber is disposed adjacent the first imaging tube and within the annular illumination tube.

Abstract: An endoscopic imaging device for endoscopy in a body vessel is disclosed. The device comprises an annular illumination tube comprising an annular illumination fiber for illuminating a body vessel during endoscopy. The device further includes a first imaging tube comprising a first imaging fiber for gross examination and navigation through the body vessel. The first imaging fiber is disposed within the annular illumination tube. The device further comprises a second imaging tube comprising a second imaging fiber for cellular imaging. The second illumination fiber is disposed adjacent the first imaging tube and within the annular illumination tube.

Abstract: An endoscopic imaging device for endoscopy in a body vessel is disclosed. The device comprises an annular illumination tube comprising an annular illumination fiber for illuminating a body vessel during endoscopy. The device further includes a first imaging tube comprising a first imaging fiber for gross examination and navigation through the body vessel. The first imaging fiber is disposed within the annular illumination tube. The device further comprises a second imaging tube comprising a second imaging fiber for cellular imaging. The second illumination fiber is disposed adjacent the first imaging tube and within the annular illumination tube.

Abstract: An endoscopic imaging device for endoscopy in a body vessel is disclosed. The device comprises an annular illumination tube comprising an annular illumination fiber for illuminating a body vessel during endoscopy. The device further includes a first imaging tube comprising a first imaging fiber for gross examination and navigation through the body vessel. The first imaging fiber is disposed within the annular illumination tube. The device further comprises a second imaging tube comprising a second imaging fiber for cellular imaging. The second illumination fiber is disposed adjacent the first imaging tube and within the annular illumination tube.