Abstract: The present invention discloses an evaluation method for acid fracturing effect based on the theory of acid-frac “stimulated zone”, comprising the following steps: establish a structured reservoir grid, and add initial artificial fractures to the structured reservoir grid; establish a fracture propagation model considering the acid-frac stimulated zone, on the basis of the structured reservoir grid, conduct numerical simulation according to the fracture propagation model, and work out the seepage parameters during acid fracturing; establish a gas well production model, and calculate the pore distribution and liquid saturation distribution of the reservoir in gas well production; calculate the cumulative production of the gas well, and calculate the multiple proportion of cumulative production increase of the construction plan according to the cumulative production of the gas well; the greater the multiple proportion, the better the acid fracturing effect.

Abstract: The present invention discloses a testing device and evaluation method for sensitivity damage of core permeability tensor, comprising the following steps: first, obtain the cubic experimental rock samples and calculate the formation pressure distribution based on the reservoir geological data; then simulate the true stress and strain state of core in formation through triaxial stress loading, design the acid liquor, alkali liquor, inorganic salt solution, formation water and different displacement rates for displacement experiments, realize the determination of core three-directional permeability tensor under the same experimental conditions through three-directional sequential displacement, process the experimental data through Darcy's law, and calculate the damage degree of core permeability tensor under different sensitivity conditions of triaxial stress state; finally, identify the real-time flow state of fluid through permeability tensor synthesis, and realize the accurate and efficient testing and evalu

Abstract: The present invention discloses a method for comprehensive evaluation of shale fracability under the geology-engineering “double-track” system, comprising the following steps: S1: Divide the target horizontal fracturing interval into multiple sampling sections; S2: Establish the reservoir property evaluation factor of each sampling section, and calculate the geological evaluation index of the target horizontal fracturing interval according to the reservoir property evaluation factor of each sampling section; S3: Establish the brittleness factor, natural fracture factor and natural fracture opening factor of each sampling section, and then establish the engineering evaluation index of each sampling section according to these factors; S4: Calculate the engineering evaluation index of the target horizontal fracturing interval according to the engineering evaluation factor of each sampling section; S5: Evaluate the fracability of the target horizontal fracturing interval according to the geological evaluation ind

Abstract: Provided are a fungus-sourced high-temperature neutral Family-45 cullulase as well as a coding gene and application thereof. The cullulase has optimal pH value of 5.5, and optimal temperature of 60° C., has certain enzyme activity in alkaline condition, and has good alkali resistance, maintains about 70% enzyme activity in optimal condition after being processed at 90° C. for 1 hour, maintains about 50% enzyme activity in optimal condition after being processed in boiling water for 1 hour, and can be well applied in c and other fields.

Abstract: Provided are a fungus-sourced high-temperature neutral Family-45 cullulase as well as a coding gene and application thereof. The cullulase has optimal pH value of 5.5, and optimal temperature of 60° C., has certain enzyme activity in alkaline condition, and has good alkali resistance, maintains about 70% enzyme activity in optimal condition after being processed at 90° C. for 1 hour, maintains about 50% enzyme activity in optimal condition after being processed in boiling water for 1 hour, and can be well applied in c and other fields.

Abstract: Provided are a fungus-sourced high-temperature acid ?-glucosidase as well as a coding gene, and an application thereof. The provide ?-glucosidase has the optimal pH value of 4.5 and the optimal temperature of 75° C., and maintains over 90% enzyme activity in the optimal condition after being processed at 60° C. for 1 h. The re-engineering yeast strain GS115/bgl3A of the coding gene comprising the ?-glucosidase has high fermentation level.

Abstract: Provided are a fungus-sourced high-temperature acid ?-glucosidase as well as a coding gene, and an application thereof. The provide ?-glucosidase has the optimal pH value of 4.5 and the optimal temperature of 75° C., and maintains over 90% enzyme activity in the optimal condition after being processed at 60° C. for 1 h. The re-engineering yeast strain GS115/bgl3A of the coding gene comprising the ?-glucosidase has high fermentation level.

Abstract: The present invention relates to the field of genetic engineering, in particular, the present invention relates to a method for producing a phytase variant with an improved thermal stability, and a phytase variant and the use thereof. The phytase variant contains at least one proline modification, compared to the phytase from Escherichia coli and other mutants thereof. The phytase variants with the modification have preferably improved properties, such as the thermal stability, optimal reaction temperature, pH property, specific activity, protease resistance and performance in animal feeds.

Abstract: The present invention relates to the field of genetic engineering, in particular, the present invention relates to a method for producing a phytase variant with an improved thermal stability, and a phytase variant and the use thereof. The phytase variant contains at least one proline modification, compared to the phytase from Escherichia coli and other mutants thereof. The phytase variants with the modification have preferably improved properties, such as the thermal stability, optimal reaction temperature, pH property, specific activity, protease resistance and performance in animal feeds.