Abstract: A cross primer isothermal amplification primer set for detecting tobacco ringspot virus, a kit and application thereof are provided. The kit and the method provided by the disclosure have good specificity and negative reaction to quarantine viruses such as southern bean mosaic virus, tomato ringspot virus, Arabidopsis mosaic virus, bean pod mottle virus and the like. The real-time fluorescence detection method has high sensitivity and can detect the RNA template of the tobacco ringspot virus with the minimum concentration of 5 ng/?L. The lowest detectable concentration of RNA template was 0.5 ng/?L. It is very suitable for on-site detection of medical and health, food safety and import and export quarantine, and is easy to be popularized and applied in a wide range.

Abstract: The automatic identification hydrogen refueling system includes a ground positioning module, a hydrogen storage module, a mechanical arm, a refueling module, an automatic identification module and an information processing module. The ground positioning module is provided with a sensor. A hydrogen conveying pipeline is connected with the refueling module. The hydrogen storage module is arranged at a tail end of the ground positioning module, and connected with the mechanical arm, the information processing module and the hydrogen conveying pipeline. The information processing module controls the mechanical arm to drive the refueling module to preliminarily align a refueling connector after receiving a sensor signal.

Abstract: The present invention discloses a primer set of LAMP-LFD detection for detecting a leaf curl virus of Melia azedaeach L. and a detection method, and belongs to the field of detection of crop diseases. The primer set of LAMP-LFD visual detection includes five primers: CLSV-F3, CLSV-B3, CLSV-FIP, CLSV-BIP and CLSV-LB and one probe primer: CLSV-Pb. The present invention also provides a visual detection method for a leaf curl virus of Melia azedaeach L., which is a combination of a loop-mediated isothermal amplification method and a lateral flow dipstick to establish an LAMP-LFD method to detect CLSV, so that the results of LAMP amplification detection are more obvious and intuitive. The present invention has the characteristics of high specificity, high sensitivity and simple operation, and is suitable for substrate and field use.

Abstract: A TPR1 gene related to a low-temperature tolerance of Pomacea, coding protein and application of the gene is disclosed. The disclosure belongs to the field of biotechnology. The present disclosure is rapid, effective and reproducible, and is an important complement to the TPR1 gene family. Pomacea is an important invasive organism, the disclosure is helpful for developing novel biological pesticide, and the low-temperature tolerance of the organism is reduced by blocking the expression of TPR1 gene in Pomacea, so as to control the further northward invasion of Pomacea. Meanwhile, by interfering with the expression of TPR1 gene, the hatching rate of Pomacea eggs can be significantly reduced, and the hatching period of eggs can be prolonged, thereby reducing the harm of Pomacea invasion.

Abstract: A compound plant-derived molluscicide for controlling Pomacea canaliculata and a preparation method and application thereof are disclosed. The compound plant-derived molluscicide is prepared from following raw materials in proportion by weight: 40-45% of water extract of Solidago canadensis L. leaves, 45-50% of water extract of Lantana camara L. roots, 0.1-0.2% of active ingredient stabilizer, and 0.1-0.2% of preservative; and a balance is ultrapure water. The disclosure has rich raw material resources, low cost, good molluscicidal effect, sustained and stable efficacy, non-toxic effect on freshwater fish, and meets the requirements of environmental protection.

Abstract: A controlling apparatus and method for an electric drive transmission used in a dual-motor electric vehicle are disclosed, wherein when the second motor is in the zero torque state, the synchronizer is shifted to a neutral position, the second motor the required torque being kept to be zero; after shifted to the neutral position, if the target gear position is the neutral position, gearshifting is completed, an if the target gear position is not the neutral position, speed control to the second motor is conducted to adjust its speed towards a target speed; once the second motor is adjusted to the target speed, the second motor is subjected to zero torque control, the second motor the required torque being zero; once the second motor comes into a zero torque state, the synchronizer is shifted to a target gear position, the required torque of the second motor being kept to be zero; once the synchronizer is located in the target gear position, the required torque of the second motor changes towards a target valu

Abstract: A hybrid system torque control method and hybrid automobile using same, the method comprising the following steps: (1) analyzing the torque required by a driver; (2) allocating and coordinating the multiple-source torque. The method ensures a consistent driving feel within the range of real-time power source torque capacity, and facilitates hybrid system matching.

Abstract: A controlling apparatus and method for an electric drive transmission used in a dual-motor electric vehicle are disclosed, wherein when the second motor is in the zero torque state, the synchronizer is shifted to a neutral position, the second motor the required torque being kept to be zero; after shifted to the neutral position, if the target gear position is the neutral position, gearshifting is completed, an if the target gear position is not the neutral position, speed control to the second motor is conducted to adjust its speed towards a target speed; once the second motor is adjusted to the target speed, the second motor is subjected to zero torque control, the second motor the required torque being zero; once the second motor comes into a zero torque state, the synchronizer is shifted to a target gear position, the required torque of the second motor being kept to be zero; once the synchronizer is located in the target gear position, the required torque of the second motor changes towards a target valu

Abstract: A hybrid system torque control method and hybrid automobile using same, the method comprising the following steps: (1) analyzing the torque required by a driver; (2) allocating and coordinating the multiple-source torque. The method ensures a consistent driving feel within the range of real-time power source torque capacity, and facilitates hybrid system matching.