Abstract: A transgenic method of obtaining blue flowers by catalyzing glutamine to synthesize indigo. The steps include: 1) respectively cloning a Sfp gene encoding phosphopantetheinyl transferase and a bpsA gene encoding indigo synthase downstream of a plant promoter in a plant-promoter-containing plasmid; 2) amplifying the obtained plasmid in E. coli and then transferring the same to Agrobacterium tumefaciens; and 3) transferring DNA containing Sfp and bpsA into a plant. The blue flowers produced by the present invention have various characteristics of natural flowers, being fresh, flower-scented, non-color-fading, and non-toxic. The transgene-encoded enzyme and the produced indigo are not in the vacuole and are not affected by the low pH of the plant vacuole, thereby resulting in a pure blue color. The precursor of the blue matter, i.e., the substrate of the enzyme, is glutamine, which is abundant in plants.

Abstract: A transgenic method of obtaining blue flowers by catalyzing glutamine to synthesize indigo is provided. The steps thereof comprise: 1) respectively cloning a Sfp gene encoding phosphopantetheinyl transferase and a bpsA gene encoding indigo synthase downstream of a plant promoter in a plant-promoter-containing plasmid; 2) amplifying the obtained plasmid in E. coli and then transferring the same to Agrobacterium tumefaciens; and 3) transferring DNA containing Sfp and bpsA into a plant. The blue flowers produced by the present invention have various characteristics of natural flowers, being fresh, flower-scented, non-color-fading, and non-toxic. The transgene-encoded enzyme and the produced indigo are not in the vacuole and are not affected by the low pH of the plant vacuole, thereby resulting in a pure blue color. The precursor of the blue matter, i.e., the substrate of the enzyme, is glutamine, which is abundant in plants.

Abstract: Provided are a tail point detection method and apparatus, a device, and a storage medium. The implementation scheme includes acquiring a target audio; identifying a sentence pattern type of the target audio; determining detection waiting duration according to the sentence pattern type; and determining a result of detecting a tail point of the target audio according to the detection waiting duration.

Abstract: Provided are a polarization imaging apparatus, a polarization imaging method, a controller and a computer readable storage medium. The polarization imaging apparatus includes an optical rotation device, a lens device, an image sensor, an image processor, and a controller which are sequentially arranged along a ray direction of incident light. The controller is configured to control the optical rotation device to be in a first optical rotation state or a second optical rotation state, control the lens device to be in an in-focus state or an out-of-focus state, and control the image sensor to collect light passing through the optical rotation device and the lens device to obtain multiple images. The image processor is configured to obtain polarized image information according to the multiple images.

Abstract: Provided are a polarization imaging apparatus, a polarization imaging method, a controller and a computer readable storage medium. The polarization imaging apparatus includes an optical rotation device, a lens device, an image sensor, an image processor, and a controller which are sequentially arranged along a ray direction of incident light. The controller is configured to control the optical rotation device to be in a first optical rotation state or a second optical rotation state, control the lens device to be in an in-focus state or an out-of-focus state, and control the image sensor to collect light passing through the optical rotation device and the lens device to obtain multiple images. The image processor is configured to obtain polarized image information according to the multiple images.