Abstract: Amylopectin potato starch with improved stability against retrogradation and improved freeze and thaw stability, wherein it contains more than 99% amylopectin, preferably 100% amylopectin, is disclosed, as well as a method for the production of a potato (Solanum tuberosum) containing said amylopectin potato starch, wherein said method involves homology-directed mutagenesis using CRISPR/nuclease technology and comprises the following steps: a) provision of potato cells or potato tissue containing potato cells, b) introduction into the nuclei of said potato cells of one or more CRISPR/nuclease complexes each comprising a specific targeting ribonucleotide sequence which is fully or essentially homologous to a target nucleotide sequence located in a DNA sequence immediately upstream of a PAM (5?-NGG-3?protospacer adjacent motif) in a gene coding for a GBSS enzyme and optionally also in a gene coding for an SSII enzyme and/or in a gene coding for an SSIII enzyme, wherein said mutagenesis takes place in one or m

Abstract: Wax esters (WE) and triacylglycerols (TAG) can be accumulated in large amounts in plant cells. The present invention relates to insect pheromone precursors which are produced as components of WE or TAG in plants. Further the invention concerns means and methods for the production of plant cells producing WE and/or TAG containing insect pheromone precursors in form of either a fatty acid or a fatty alcohol. Said method uses plants where wax esters are modified by desaturase, fatty acyl reductase and wax ester synthase or the triacylglycerol is modified by a desaturase. Finally the insect pheromone precursors can be extracted and used in the manufacturing of insect pheromones from WE and TAG.

Abstract: Wax esters (WE) and triacylglycerols (TAG) can be accumulated in large amounts in plant cells. The present invention relates to insect pheromone precursors which are produced as components of WE or TAG in plants. Further the invention concerns means and methods for the production of plant cells producing WE and/or TAG containing insect pheromone precursors in form of either a fatty acid or a fatty alcohol. Said method uses plants where wax esters are modified by desaturase, fatty acyl reductase and wax ester synthase or the triacylglycerol is modified by a desaturase. Finally the insect pheromone precursors can be extracted and used in the manufacturing of insect pheromones from WE and TAG.

Abstract: Amylopectin potato starch with improved stability against retro-gradation and improved freeze and thaw stability, wherein it contains more than 99% amylopectin, preferably 100% amylopectin, is disclosed, as well as a method for the production of a potato (Solanum tuberosum) containing said amylopectin potato starch, wherein said method involves homology-directed mutagenesis using CRISPR/nuclease technology and comprises the following steps: a) provision of potato cells or potato tissue containing potato cells, b) introduction into the nuclei of said potato cells of one or more CRISPR/nuclease complexes each comprising a specific targeting ribonucleotide sequence which is fully or essentially homologous to a target nucleotide sequence located in a DNA sequence immediately upstream of a PAM (5?-NGG-3?protospacer adjacent motif) in a gene coding for a GBSS enzyme and optionally also in a gene coding for an SSII enzyme and/or in a gene coding for an SSIII enzyme, wherein said mutagenesis takes place in one or mor

Abstract: Wax esters (WE) and triacylglycerols (TAG) can be accumulated in large amounts in plant cells. The present invention relates to insect pheromone precursors which are produced as components of WE or TAG in plants. Further the invention concerns means and methods for the production of plant cells producing WE and/or TAG containing insect pheromone precursors in form of either a fatty acid or a fatty alcohol. Said method uses plants where wax esters are modified by desaturase, fatty acyl reductase and wax ester synthase or the triacylglycerol is modified by a desaturase. Finally the insect pheromone precursors can be extracted and used in the manufacturing of insect pheromones from WE and TAG.

Abstract: The present invention relates to plant cells and plants, which are genetically modified, whereby the genetic modification leads to an alteration of storage compound deposition in Beta vulgaris tap-root, such as sugar beet tap-root or fodder beet tap-root. By the alteration the tap-root of the plants accumulates starch in comparison with the corresponding wild type plant tap-root. In addition, the present invention concerns means and methods for the manufacture of such plant cells and plants. The present invention also concerns the starches synthesised from the tap-root of these plant, methods for manufacturing these starch. Furthermore, the present invention also relates to nucleic acids, coding the genes involved in the synthesis of starch, vectors, host cells, plant cells, and plants containing such nucleic acid molecules.

Abstract: This invention relates to transgenic potato plants producing amylopectin-type starch with enhanced retrogradation stability. The invention further provides a method for producing and identifying such transgenic potato plants.

Abstract: The present invention relates to the use of mutated AHAS genes conferring resistance to herbicides and resulting in an highly efficient selection system for the production of transgenic potato lines. The invention provides a key advantage by minimizing the escapes to almost zero of all shoots regenerated without interfering with the high number of shoots regenerated per explant.

Abstract: The invention relates to methods for increasing the amylose content in plants, preferably in potato plants, by expressing a starch biosynthesis enhancing protein. The invention furthermore relates to an expression cassette expressing the polypeptide in potato plants, preferably in the tubers, the transgenic plants expressing the polypeptide and to the use of said transgenic plants for the production of fine chemicals especially other than native starches.

Abstract: Genetically engineered modification of potato for suppressing the formation of amylose-type starch is described. Three fragments for insertion in the antisense direction into the potato genome are also described. Moreover, antisense constructs, genes and vectors comprising said antisense fragments are described. Further a promoter for the gene coding for formation of granule-bound starch synthase and also the gene itself are described. Also cells, plants, tubers, microtubers and seeds of potato comprising said antisense fragments are described. Finally, amylopectin-type starch, both native and derivatised, derived from the potato that is modified in a genetically engineered manner, as well as a method of suppressing amylose formation in potato are described.

Abstract: Genetically engineered modification of potato for suppressing formation of amylopectin-type starch is described.The invention describes an antisense construct for inhibiting, to a varying extent, the expression of the gene coding for formation of branching enzyme (BE gene) in potato, said antisense construct comprising a tuber-specific promoter, transcription start and the first exon of the BE gene, inserted in the antisense direction.Also cells, plants, tubers, microtubers and seeds of potato comprising said antisense construct are described.Finally, amylose-type starch, both native and derivatized, derived from the potato that is modified in a genetically engineered manner, as well as a method of suppressing amylopectin formation in potato are described.

Abstract: Genetically engineered modification of potato for suppressing formation of amylose-type starch is described.Three fragments for insertion in the antisense direction into the potato genome are also described. Moreover, antisense constructs, genes and vectors comprising said antisense fragments are described. Further a promoter for the gene coding for formation of granule-bound starch synthase and also the gene itself are described.Also cells, plants, tubers, microtubers and seeds of potato comprising said antisense construct are described.Finally, amylopectin-type starch, both native and derivatized, derived from the potato that is modified in a genetically engineered manner, as well as a method of suppressing amylose formation in potato are described.