Abstract: Disclosed are compositions, methods, and systems for genetic identification and detection of low-copy number nucleic acids and low-copy number of proteins, and provides method, compositions, and kits useful for this purpose. The methods of the invention can be used to detect in a given sample the presence of low-copy number of nucleic acids (e.g., DNA or RNA) and low-copy number of protein from various microorganisms, including SARS-COV-2.

Abstract: Disclosed are compositions, methods, and systems for genetic identification and detection of low-copy number nucleic acids, and provides method, compositions, and kits useful for this purpose. The methods of the invention can be used to detect in a given sample the presence of low-copy number of nucleic acids (e.g., DNA or RNA) of various microorganisms, including SARS-COV-2.

Abstract: The present teachings relate to methods of purifying, isolating, separating, and identifying target nucleic acids. In some embodiments of the present teachings, an affinity moiety can be incorporated into one of the flanking primers of a nucleic acid amplification reaction primer pair. The reaction mixture can be contacted with a binding moiety specific for the affinity moiety, thereby allowing immobilization of the double stranded amplification product, separation of reaction components lacking the affinity moiety, and isolation of the target nucleic acid strand. Further, one of the flanking primers of the nucleic acid amplification reaction can comprise a label and a mobility modifier, thereby facilitating identification of the target nucleic acids. In some embodiments, the amplification reaction is multiplexed and comprises polymorphic microsatellites useful in human identification. and the manufacturing of molecular size standards.