Patents by Inventor Perry Frey
Perry Frey has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7456271Abstract: There are provided nucleic acids, including isolated DNA molecules, which encode glutamate 2,3-aminomutase enzymes, polypeptides produced from such nucleic acids and methods of making the nucleic acids and polypeptides. There are further provided methods of producing ?-glutamate from glutamate using glutamate 2,3-aminomutase.Type: GrantFiled: October 11, 2006Date of Patent: November 25, 2008Assignee: Wisconsin Alumni Research FoundationInventors: Perry A. Frey, Frank J. Ruzicka
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Patent number: 7452701Abstract: Purified ?-amino acids are of considerable interest in the preparation of pharmacologically active compounds and industrial precursors. Although enantiomerically pure ?-amino acids can be produced by standard chemical synthesis, this traditional approach is time consuming, requires expensive starting materials, and results in a racemic mixture which must be purified further. However, DNA molecules encoding lysine 2,3-aminomutase can be used to prepare ?-amino acids by methods that avoid the pitfalls of chemical synthesis. The present invention provides a method of producing enantiomerically pure ?-amino acids from ?-amino acids comprising catalyzing the conversion of an ?-amino acid to a corresponding ?-amino acid by utilizing a lysine 2,3-aminomutase as the catalyst.Type: GrantFiled: September 5, 2002Date of Patent: November 18, 2008Assignee: Wisconsin Alumni Research FoundationInventors: Perry A. Frey, Frank J. Ruzicka
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Publication number: 20080268510Abstract: Purified ?-amino acids are of considerable interest in the preparation of pharmacologically active compounds. Although enantiomerically pure ?-amino acids, such as L-?-lysine, can be produced by standard chemical synthesis, this traditional approach is time consuming, requires expensive starting materials, and results in a racemic mixture which must be purified further. However, DNA molecules encoding lysine 2,3-aminomutase can be used to prepare L-?-lysine by methods that avoid the pitfalls of chemical synthesis. In particular, L-?-lysine can be synthesized by cultures of host cells that express recombinant lysine 2,3-aminomutase. Alternatively, such recombinant host cells can provide a source for isolating quantities of lysine 2,3-aminomutase, which in turn, can be used to produce L-?-lysine in vitro.Type: ApplicationFiled: November 13, 2007Publication date: October 30, 2008Inventors: Perry A. Frey, Frank J. Ruzicka
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Patent number: 7354745Abstract: Purified ?-amino acids are of considerable interest in the preparation of pharmacologically active compounds. Although enantiomerically pure ?-amino acids, such as L-?-lysine, can be produced by standard chemical synthesis, this traditional approach is time consuming, requires expensive starting materials, and results in a racemic mixture which must be purified further. However, DNA molecules encoding lysine 2,3-aminomutase can be used to prepare L-?-lysine by methods that avoid the pitfalls of chemical synthesis. In particular, L-?-lysine can be synthesized by cultures of host cells that express recombinant lysine 2,3-aminomutase. Alternatively, such recombinant host cells can provide a source for isolating quantities of lysine 2,3-aminomutase, which in turn, can be used to produce L-?-lysine in vitro.Type: GrantFiled: May 1, 2001Date of Patent: April 8, 2008Assignee: Wisconsin Alumni Research FoundationInventors: Perry A. Frey, Frank J. Ruzicka
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Publication number: 20070092952Abstract: There are provided nucleic acids, including isolated DNA molecules, which encode glutamate 2,3-aminomutase enzymes, polypeptides produced from such nucleic acids and methods of making the nucleic acids and polypeptides. There are further provided methods of producing ?-glutamate from glutamate using glutamate 2,3-aminomutase.Type: ApplicationFiled: October 11, 2006Publication date: April 26, 2007Inventors: Perry Frey, Frank Ruzicka
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Publication number: 20030113882Abstract: Purified &bgr;-amino acids are of considerable interest in the preparation of pharmacologically active compounds and industrial precursors. Although enantiomerically pure &bgr;-amino acids can be produced by standard chemical synthesis, this traditional approach is time consuming, requires expensive starting materials, and results in a racemic mixture which must be purified further. However, DNA molecules encoding lysine 2,3-aminomutase can be used to prepare &bgr;-amino acids by methods that avoid the pitfalls of chemical synthesis. The present invention provides a method of producing enantiomerically pure &bgr;-amino acids from &agr;-amino acids comprising catalyzing the conversion of an &agr;-amino acid to a corresponding &bgr;-amino acid by utilizing a lysine 2,3-aminomutase as the catalyst.Type: ApplicationFiled: September 5, 2002Publication date: June 19, 2003Applicant: Wisconsin Alumni Research FoundationInventors: Perry A. Frey, Frank J. Ruzicka
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Publication number: 20020173637Abstract: Purified &bgr;-amino acids are of considerable interest in the preparation of pharmacologically active compounds. Although enantiomerically pure &bgr;-amino acids, such as L-&bgr;-lysine, can be produced by standard chemical synthesis, this traditional approach is time consuming, requires expensive starting materials, and results in a racemic mixture which must be purified further. However, DNA molecules encoding lysine 2,3-aminomutase can be used to prepare L-&bgr;-lysine by methods that avoid the pitfalls of chemical synthesis. In particular, L-&bgr;-lysine can be synthesized by cultures of host cells that express recombinant lysine 2,3-aminomutase. Alternatively, such recombinant host cells can provide a source for isolating quantities of lysine 2,3-aminomutase, which in turn, can be used to produce L-&bgr;-lysine in vitro.Type: ApplicationFiled: May 1, 2001Publication date: November 21, 2002Applicant: Wisconsin Alumni Research Foundation (WARF).Inventors: Perry A. Frey, Frank J. Ruzicka
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Patent number: 6248874Abstract: Purified &bgr;-amino acids are of considerable interest in the preparation of pharmacologically active compounds. Although enantiomerically pure &bgr;-amino acids, such as L-&bgr;-lysine, can be produced by standard chemical synthesis, this traditional approach is time consuming, requires expensive starting materials, and results in a racemic mixture which must be purified further. However, DNA molecules encoding lysine 2,3-aminomutase can be used to prepare L-&bgr;-lysine by methods that avoid the pitfalls of chemical synthesis. In particular, L-&bgr;-lysine can be synthesized by cultures of host cells that express recombinant lysine 2,3-aminomutase. Alternatively, such recombinant host cells can provide a source for isolating quantities of lysine 2,3-aminomutase, which in turn, can be used to produce L-&bgr;-lysine in vitro.Type: GrantFiled: June 11, 1999Date of Patent: June 19, 2001Assignee: Wisconsin Alumni Research FoundationInventors: Perry A. Frey, Frank J. Ruzicka
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Patent number: 4728730Abstract: A method for preparing sulfur and/or selenium containing phosphoanhydrides that have at least a triphosphate moiety is disclosed, together with a cyclo-intermediate formed during the reaction. The method includes reacting a first compound having an available and reactive group selected from phosphorodihalidate, thiophosphorodihalidate, and selenic phosphorodihalidate (the compound having a remaining portion that does not interfere with the reaction), with a second compound having an available and reactive group selected from phosphate, thiophosphate, and selenic phosphate (the second compound also having a remaining segment that does not interfere with the reaction). The selection of the reactive groups is such that at least one of the reactive groups is the thio or selenic variant, and the selection of the remaining portion and segment are such that at least one is attached to a linking group selected from phosphate, thiophosphate, and selenic phosphate that links it to the available and reactive group.Type: GrantFiled: September 12, 1984Date of Patent: March 1, 1988Assignee: Wisconsin Alumini Research FoundationInventors: Perry A. Frey, Hsu-Tso Ho