Patents by Inventor Perry G. Caimi

Perry G. Caimi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Xylose utilization in recombinant zymomonas
    Patent number: 8679822
    Abstract: Xylose-utilizing Zymomonas strains studied were found to accumulate ribulose when grown in xylose-containing media. Engineering these strains to increase ribose-5-phosphate isomerase activity led to reduced ribulose accumulation, improved growth, improved xylose utilization, and increased ethanol production.
    Type: Grant
    Filed: June 16, 2011
    Date of Patent: March 25, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventors: Perry G. Caimi, Laura McCole, Luan Tao, Jean-Francois Tomb, Paul V. Viitanen
  • Xylose utilizing with improved ethanol production in biomass hydrolysate medium
    Patent number: 8569458
    Abstract: Xylose-utilizing, ethanol producing strains of Zymomonas mobilis with improved performance in medium comprising biomass hydrolysate were isolated using an adaptation process. Independently isolated strains were found to have independent mutations in the same coding region. Mutation in this coding may be engineered to confer the improved phenotype.
    Type: Grant
    Filed: March 12, 2013
    Date of Patent: October 29, 2013
    Assignee: E I du Pont de Nemours and Company
    Inventors: Perry G. Caimi, William D Hitz, Paul V Viitanen, Barry Stieglitz
  • Xylose utilizing with improved ethanol production in biomass hydrolysate medium
    Patent number: 8476048
    Abstract: Xylose-utilizing, ethanol producing strains of Zymomonas mobilis with improved performance in medium comprising biomass hydrolysate were isolated using an adaptation process. Independently isolated strains were found to have independent mutations in the same coding region. Mutation in this coding may be engineered to confer the improved phenotype.
    Type: Grant
    Filed: December 12, 2011
    Date of Patent: July 2, 2013
    Assignee: E I du Pont de Nemours and Company
    Inventors: Perry G Caimi, William D Hitz, Barry Stieglitz, Paul V Viitanen
  • with improved xylose utilization in stress conditions
    Patent number: 8465961
    Abstract: Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
    Type: Grant
    Filed: July 3, 2012
    Date of Patent: June 18, 2013
    Assignees: E I du Pont de Nemours and Company
    Inventors: Perry G Caimi, Mark Emptage, Xu Li, Paul V Viitanen, Yat-Chen Chou, Mary Ann Franden, Min Zhang
  • Fructan biosynthetic enzymes
    Patent number: 8399738
    Abstract: This invention relates to isolated nucleic acid fragments encoding fructosyltransferases. More specifically, this invention relates to polynucleotides encoding 1-FFTs, 6-SFTs, or 1-SSTs. The invention also relates to the construction of a recombinant DNA constructs encoding all or a portion of the fructosyltransferases, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the fructosyltransferases in a transformed host cell.
    Type: Grant
    Filed: March 30, 2012
    Date of Patent: March 19, 2013
    Assignee: E I du Pont de Nemours and Company
    Inventors: Johan M. Stoop, Stephen M. Allen, Perry G. Caimi
  • XYLOSE UTILIZING ZYMOMONAS MOBILIS WITH IMPROVED ETHANOL PRODUCTION IN BIOMASS HYDROLYSATE MEDIUM
    Publication number: 20120329114
    Abstract: Xylose-utilizing, ethanol producing strains of Zymomonas mobilis with improved performance in medium comprising biomass hydrolysate were isolated using an adaptation process. Independently isolated strains were found to have independent mutations in the same coding region. Mutation in this coding may be engineered to confer the improved phenotype.
    Type: Application
    Filed: December 12, 2011
    Publication date: December 27, 2012
    Applicant: E.I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Perry G. Caimi, William D. Hitz
  • ZYMOMONAS WITH IMPROVED XYLOSE UTILIZATION IN STRESS CONDITIONS
    Publication number: 20120276607
    Abstract: Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
    Type: Application
    Filed: July 3, 2012
    Publication date: November 1, 2012
    Applicant: E. I. DU PONT DE NEMOURS AND COMPANY & ALLIANCE FOR SUSTAINABLE ENERGY LLC
    Inventors: PERRY G. CAIMI, MARK EMPTAGE, XU LI, PAUL V. VIITANEN, YAT-CHEN CHOU, MARY ANN FRANDEN, MIN ZHANG
  • with improved xylose utilization in stress conditions
    Patent number: 8247208
    Abstract: Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
    Type: Grant
    Filed: December 18, 2009
    Date of Patent: August 21, 2012
    Assignees: E I du Pont de Nemours and Company
    Inventors: Perry G. Caimi, Mark Emptage, Xu Li, Paul V. Viitanen, Yat-Chen Chou, Mary Ann Franden, Min Zhang
  • FRUCTAN BIOSYNTHETIC ENZYMES
    Publication number: 20120185966
    Abstract: This invention relates to isolated nucleic acid fragments encoding fructosyltransferases. More specifically, this invention relates to polynucleotides encoding 1-FFTs, 6-SFTs, or 1-SSTs. The invention also relates to the construction of a recombinant DNA constructs encoding all or a portion of the fructosyltransferases, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the fructosyltransferases in a transformed host cell.
    Type: Application
    Filed: March 30, 2012
    Publication date: July 19, 2012
    Applicant: E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Johan M. Stoop, Stephen M. Allen, Perry G. Caimi, Karlene H. Butler
  • XYLOSE UTILIZATION IN RECOMBINANT ZYMOMONAS
    Publication number: 20120156746
    Abstract: Xylose-utilizing Zymomonas strains studied were found to accumulate ribulose when grown in xylose-containing media. Engineering these strains to increase ribose-5-phosphate isomerase activity led to reduced ribulose accumulation, improved growth, improved xylose utilization, and increased ethanol production.
    Type: Application
    Filed: June 16, 2011
    Publication date: June 21, 2012
    Inventors: Perry G. Caimi, Laura McCole, Luan Tao, Jean-Francois Tomb, Paul V. Viitanen
  • Fructan biosynthetic enzymes
    Patent number: 8168862
    Abstract: This invention relates to isolated nucleic acid fragments encoding fructosyltransferases. More specifically, this invention relates to polynucleotides encoding 1-FFTs, 6-SFTs, or 1-SSTs. The invention also relates to the construction of a recombinant DNA constructs encoding all or a portion of the fructosyltransferases, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the fructosyltransferases in a transformed host cell.
    Type: Grant
    Filed: January 21, 2011
    Date of Patent: May 1, 2012
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Johan M. Stoop, Stephen M. Allen, Perry G. Caimi, Karlene H. Butler
  • Zymomonas with improved xylose utilization
    Patent number: 7998722
    Abstract: Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.
    Type: Grant
    Filed: March 25, 2009
    Date of Patent: August 16, 2011
    Assignees: E. I. du Pont de Nemours and Company, Alliance for Sustainable Energy LLC
    Inventors: Paul V. Viitanen, Luan Tao, Yuying Zhang, Perry G. Caimi, Carol M. McCutchen, Laura McCole, Min Zhang, Yat-Chen Chou, Mary Ann Franden
  • High expression promoters
    Patent number: 7989206
    Abstract: Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.
    Type: Grant
    Filed: March 25, 2009
    Date of Patent: August 2, 2011
    Assignee: E.I. du Pont de Nemours and Company Alliance for Sustainable Energy LLC
    Inventors: Paul V. Viitanen, Luan Tao, Yuying Zhang, Perry G. Caimi, Laura McCole, Min Zhang, Yat-Chen Chou, Carol M. McCutchen, Mary Ann Franden
  • FRUCTAN BIOSYNTHETIC ENZYMES
    Publication number: 20110119787
    Abstract: This invention relates to isolated nucleic acid fragments encoding fructosyltransferases. More specifically, this invention relates to polynucleotides encoding 1-FFTs, 6-SFTs, or 1-SSTs. The invention also relates to the construction of a recombinant DNA constructs encoding all or a portion of the fructosyltransferases, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the fructosyltransferases in a transformed host cell.
    Type: Application
    Filed: January 21, 2011
    Publication date: May 19, 2011
    Applicant: E.I. DU PONT DE NEMOURS AND COMPANY
    Inventors: JOHAN M. STOOP, Stephen M. Allen, Perry G. Caimi, Karlene H. Butler
  • with improved ethanol production in medium containing concentrated sugars and acetate
    Patent number: 7897396
    Abstract: Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas strains further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in mixed-sugars medium comprising xylose, and, in particular, in the presence of acetate.
    Type: Grant
    Filed: October 30, 2008
    Date of Patent: March 1, 2011
    Assignees: E.I. du Pont de Nemours and Company
    Inventors: Perry G. Caimi, Yat-Chen Chou, Mary Ann Franden, Kyle Knoke, Luan Tao, Paul V. Viitanen, Min Zhang, Yuying Zhang
  • ZYMOMONAS WITH IMPROVED XYLOSE UTILIZATION IN STRESS CONDITIONS
    Publication number: 20110014670
    Abstract: Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
    Type: Application
    Filed: December 18, 2009
    Publication date: January 20, 2011
    Applicant: E.I. DU PONT DE NEMOURS AND COMPANY & ALLIANCE FOR SUSTAINABLE ENERGY LLC
    Inventors: PERRY G. CAIMI, Mark Emptage, Xu Li, Paul V. Viitanen, Yat-Chen Chou, Mary Ann Franden, Min Zhang
  • with improved ethanol production in medium containing concentrated sugars and acetate
    Patent number: 7803623
    Abstract: Through screening of a Zymomonas mutant library the himA gene was found to be involved in the inhibitory effect of acetate on Zymomonas performance. Xylose-utilizing Zymomonas further engineered to reduce activity of the himA gene were found to have increased ethanol production in comparison to a parental strain, when cultured in medium comprising xylose and acetate.
    Type: Grant
    Filed: October 30, 2008
    Date of Patent: September 28, 2010
    Assignees: E.I. du Pont de Nemours and Company, Alliance for Sustainable Energy LLC
    Inventors: Perry G. Caimi, Yat-Chen Chou, Mary Ann Franden, Kyle Knoke, Luan Tao, Paul V. Viitanen, Min Zhang, Yuying Zhang
  • Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas
    Patent number: 7741084
    Abstract: Production of ethanol using a strain of xylose-utilizing Zymomonas with a genetic modification of the glucose-fructose oxidoreductase gene was found to be improved due to greatly reduced production of xylitol, a detrimental by-product of xylose metabolism synthesized during fermentation.
    Type: Grant
    Filed: September 27, 2007
    Date of Patent: June 22, 2010
    Assignees: E. I. du Pont de Nemours and Company, Alliance for Sustainable Energy LLC
    Inventors: Paul V. Viitanen, Carol M. McCutchen, Mark Emptage, Perry G. Caimi, Min Zhang, Yat-Chen Chou
  • PLANT GALACTINOL SYNTHASE HOMOLOGS
    Publication number: 20100077510
    Abstract: Isolated nucleic acid fragments encoding galactinol synthase are disclosed. Recombinant DNA construct(s) for use in altering expression of endogenous genes encoding galactinol synthase are also disclosed.
    Type: Application
    Filed: November 24, 2009
    Publication date: March 25, 2010
    Applicant: E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Johan M. Stoop, Stephen M. Allen, Perry G. Caimi
  • Ethanol production in fermentation of mixed sugars containing xylose
    Patent number: 7629156
    Abstract: Xylose-utilizing Z. mobilis strains were found to have improved ethanol production when grown in medium containing mixed sugars including xylose if sorbitol or mannitol was included in the medium. The effect was seen in concentrations of mixed sugars where no growth lag period occurs, as well as in higher sugars concentrations.
    Type: Grant
    Filed: September 27, 2007
    Date of Patent: December 8, 2009
    Assignees: E.I. du Pont de Nemours and Company, Alliance For Sustainable Energy, LLC
    Inventors: Paul V. Viitanen, Carol M. Mc Cutchen, Xu Li, Mark Emptage, Perry G. Caimi, Min Zhang, Yat-Chen Chou, Mary Ann Franden