Abstract: The invention relates to a method of increasing the yield of virus, virus particles, or viral vectors from host cells in a bioreactor. The invention provides a reproducible and robust method and system of determining and controlling the optimal time of infection of host cells using a correlation of process air parameters including Air flow, O2 flow, and respective trends thereof resulting in increased virus yield.

Abstract: The invention relates to a method of increasing the yield of virus, virus particles, or viral vectors from host cells in a bioreactor. The invention provides a reproducible and robust method and system of determining and controlling the optimal time of infection of host cells using a correlation of process air parameters including Air flow, O2 flow, and respective trends thereof resulting in increased virus yield.

Abstract: A temporary clamping fastener is disclosed, and includes a bushing having an inner cavity, an upper open end, and a lower open end. The temporary clamping fastener also includes a pull shaft having an elongated body and a slot extending through the elongated body. A portion of the pull shaft is disposed in the inner cavity of the bushing, and the pull shaft is configured to linearly translate relative to the bushing. The temporary clamping fastener also includes a clamp blade disposed within the slot of the pull shaft and rotatable between a stowed position where the clamp blade is housed within the slot and a deployed position and a portion of the clamp blade extends from the slot of the pull shaft to create an engagement surface for through-hole clamping.

Abstract: The invention relates to a method of increasing the yield of virus, virus particles, or viral vectors from host cells in a bioreactor. The invention provides a reproducible and robust method and system of determining and controlling the optimal time of infection of host cells using a correlation of process air parameters including Air flow, O2 flow, and respective trends thereof resulting in increased virus yield.

Abstract: A temporary clamping fastener is disclosed, and includes a bushing having an inner cavity, an upper open end, and a lower open end. The temporary clamping fastener also includes a pull shaft having an elongated body and a slot extending through the elongated body. A portion of the pull shaft is disposed in the inner cavity of the bushing, and the pull shaft is configured to linearly translate relative to the bushing. The temporary clamping fastener also includes a clamp blade disposed within the slot of the pull shaft and rotatable between a stowed position where the clamp blade is housed within the slot and a deployed position and a portion of the clamp blade extends from the slot of the pull shaft to create an engagement surface for through-hole clamping.

Abstract: A leak detection system configured to be attached on an exterior of a conduit to detect a leak in a non-pressurized conduit. The leak detection system includes a first fluid section and a second fluid section that each includes the same or similar fluid volumes. The first fluid section includes a testing chamber configured be attached to the conduit. With the testing chamber positioned on the conduit, a pressure sensor connected to each of the first and second fluid sections senses a difference in the fluid pressure between the first and second fluid sections to detect a leak.

Abstract: A leak detection system configured to be attached on an exterior of a conduit to detect a leak in a non-pressurized conduit. The leak detection system includes a first fluid section and a second fluid section that each includes the same or similar fluid volumes. The first fluid section includes a testing chamber configured be attached to the conduit. With the testing chamber positioned on the conduit, a pressure sensor connected to each of the first and second fluid sections senses a difference in the fluid pressure between the first and second fluid sections to detect a leak.

Abstract: A method of purifying retroviral vector particles which comprises culturing retroviral vector producer cells which are capable of generating retroviral vector particles, and obtaining a supernatant containing retroviral vector particles from the culture of retroviral vector producer cells. The supernatant is clarified, and then concentrated. The supernatant then is contacted with a precipitation agent, and the supernatant and precipitation agent are centrifuged. A precipitate is recovered, resuspended, and subjected to a high-speed centrifugation. Retroviral particles then are recovered. The above method provides for increased recovery of retroviral vector particles which may be employed in gene therapy.

Abstract: The present invention relates to an efficient method, termed spin transduction, of transducing cells with retroviral vectors. Retroviral particles, suspended in a pharmaceutically acceptable carrier, are contacted with the cells to be transduced, and the mixture is centrifuged for a sufficient period of time to permit transduction. This method leads to a 3- to 15-fold increase in transduction efficiency, depending on the type of cells transduced.